Seminar Series Kick-Off 9 September 2016 New Resources and Opportunities Johns Hopkins Malaria Research Institute Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health
History and Goals Since 2001 Bloomberg Philanthropies has given $125 million to the Bloomberg School to fund a state-of-the-art research facility that would mount a broad program of basic-science research to treat and control malaria, develop a vaccine and find new drug targets to prevent and cure this deadly disease. Diane Griffin, MD, PhD Founding Director 2
JHMRI Leadership Deputy Directors Director Marcelo Jacobs-Lorena, PhD Peter Agre, MD George Dimopoulos, PhD, MBA Professor Bloomberg Professor Distinguished Professor Photini Sinnis, MD Fidel Zavala, MD Professor Professor 3
2016 Scientific Advisory Board The external Scientific Advisory Board meets annually to evaluate Malaria Research Institute activity. • Daniel E. Goldberg, MD, PhD, Professor of Medicine & Molecular Microbiology at Washington University • Diane Griffin, MD, PhD, Professor and Chair, Department of Molecular Microbiology & Immunology at JHBSPH and Founding Director of JHMRI • Thomas Kelly, MD, PhD, Director, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center • Michael Klag, MD, MPH, Dean, JHBSPH and Chair of the External Advisory Board • Gregory Lanzaro, PhD, Director, University of California Mosquito Research Program, UC Davis • Bernard Roizman, ScD, Professor, Departments of Microbiology, and Molecular Genetics & Cell Biology, University of Chicago • Eric Schoomaker, MD, PhD, LTG USA (Retired) Professor, Office of the President, Uniformed Services University of the Health Sciences • Alfred Sommer, MD, MHS, Professor and Dean Emeritus, JHBSPH • Allan Spradling, PhD , Director of the Department of Embryology, Carnegie Institution of Washington • Kathryn Zoon, PhD , Director, National Institutes of Health Division of Intramural Research 4
Malaria Life Cycle Mosquito 5
Malaria Researchers affiliated with the JHMRI Fidel Zavala Monique Stins Phil Thuma Jenny Stevenson Douglas Norris Photini Sinnis Immunology Cerebral malaria Clinical malaria Entomology Mosquito biology/ecology Cell Biology Gary Ketner Deborah Andrew Immunoprophylaxis Salivary gland biology Richard Markham Conor McMeniman Vaccine platform Mosquito olfaction Isabelle Coppens Chris Potter Plasmodium metabolism Sensory/neuronal function Prakash Srinivasan Marcelo Jacobs-Lorena Host-pathogen interactions Mosquito-parasite interactions and vaccine development George Dimopoulos Peter Agre Mosquito innate immunity Water channel biology David Sullivan William Moss Heme metabolism Epidemiology Sean Prigge Clive Shiff Sungano Mharakurwa Theresa Shapiro Drug targets Parasite diagnostics Drug development Passive case detection 6
Macha Research Trust Southern Province, Zambia Field station in rural Zambia Molecular laboratory Insectary “Mosquito House” 7
JHBSPH Faculty Based in Macha Dr. Jennifer Stevenson , PhD Dr. Phil Thuma , MD Senior Scientific Advisor Lead Entomologist 8
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Core Facilities I. JHMRI Cores • Biophysics Core • Environmental Surveillance Core • Insectary Core • Parasitology Core II. JHU & MMI Cores Facilities https://johnshopkins.corefacilities.org • Genomic Analysis and Sequencing Facility • Microscopy Facility • Flow Cytometry &Cell Sorting/Becton Dickinson Immune Function Lab 10
Biophysics Core Facility Co-Directors Scott Bailey, PhD Email: scbailey@jhsph.edu Sean Prigge, PhD Email: sprigge@jhsph.edu Biophysics Core Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room W8620 Baltimore, Maryland 21205 Telephone: 443-287-48227 fax: 410-955-0105 11
Biophysics Core The Biophysics Core Facility provides equipment for JHMRI investigators who want to characterize macromolecules or macromolecular complexes using biophysical techniques Isothermal Titration Calorimetry: This technique measures the heat of binding between two molecules, providing a direct measurement of binding affinity. Equipment: MicroCal VP-ITC Location: W8620
Biophysics Core Dynamic Light Scattering. This technique provides information about the size, shape and oligomerization state of biomolecules. Equipment: Proterion DynaPro with Peltier temperature control Location: W8620
Biophysics Core Circular Dichroism. This technique measures the overall secondary structure content of proteins and quantifies changes in structure under different conditions. Equipment: Jasco J-810 Spectropolarimeter with Peltier temperature control, automated dual titration system, and scanning emission monochromator Location: W8620
Environmental Surveillance Core Facility Director Timothy Shields, MA Department of Epidemiology Email: tshields@jhu.edu Facility Co-Director Frank Curriero, PhD Department of Epidemiology Email: fcurriero@jhu.edu Environmental Surveillance Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room E3025 Baltimore, Maryland 21205 Telephone: 410-502-9077 fax: 410-955-0105
Overview - Spatial Analysis Spatial Data GIS* Spatial Statistics Obtain, Collect, Create Maps Quantify Relationships Images (Satellite, Google Earth) Spatial Database/Integration Test Hypothesis Spatial Data Accuracy View Patterns Simultaneously Study Design Non-spatial data Generate/Refine Hypothesis Go Beyond the Map! Rationale Identified malaria outcomes and measured environmental determinants likely vary spatially. Collect and harness this information to improve • study design • analysis • prevention, intervention, control and policy efforts *Geographic Information System
Spatial Data - Satellite Imagery - Geography, Hydrology - Land Use/Land Cover - Climate, Census - GPS’d locations - Outcome locations 17
GIS New Analysis Opportunities Integration, Hypothesis Generation
- Risk map estimation - Identify Env factors Spatial Statistics - Assess clustering - Trends over space & time Choma Mutasa Nchelenge - Detect clusters - Movement over space & time GPS Logged Movement Gambiae Counts Dry Rainy
The JHMRI Insect Core Facility DESCRIPTION INSECTARY MAP Location : SPH 4 th floor, Room • W4700 • Size: 3000 sq ft. divided into two operational areas – High (Area 2 ) and Low containment (Areas 1, 3 & 4 ). • There are seven procedure rooms and an autoclave.
Walk-in incubator for mosquito rearing • 7 environmentally controlled walk-in incubators for mosquito rearing. • 9 reach-in incubators whose temperature and humidity can be custom adjusted.
Services • Mosquito rearing : An gambiae and An. stephensi • Provide space and assistance for rearing of other mosquito species . • Training: insectary use, mosquito rearing, handling and identification techniques. • Mosquito transformation ( transgenic mosquitoes
For new insectary users: • Need orientation training before access to the insectary is granted. • Need specialized training to work with Plasmodium falciparum -infected mosquitoes. • Place orders: http://jhmalaria.jhsph.edu/department/insectary /FrameForm.cfm
Contacts • Facility Director: Dr. Marcelo Jacobs-Lorena School of Public Health Rm E4632 Tel: 443-287-0839 Email: ljacob13@jhu.edu • Facility Manager: Mr. Christopher Kizito School of Public Health Rm W4008 Tel: 443-287-0789 Email: ckizito1@jhu.edu
Parasite Core Facility http://www.parasitecore.org/ Facility Director: George Dimopoulos, PhD, MBA gdimopo1@jhu.edu Facility Co-Manager: Godfree Mlambo, PhD gmlambo1@jhu.edu Facility Co-Manager: Abhai K. Tripathi, PhD atripat2@jhu.edu Parasite Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room W4212 Baltimore, Maryland 21205 Telephone: 410-502-7744 fax: 410-955-0105
Parasite Core Facility Location -TISSUE/CELL/PARASITE CULTURE ROOM W4214 -OFFICE AND MOLECULAR LAB ON 5 TH FLOOR, W5315
Parasite Core Facility Major Core Services… P. falciparum Parasite Cultures Asexual Stage (1 week prep time) Gametocyte Stage (3 week prep time) Rings Stage Mosquito Stage Parasites P. falciparum sporozoites (6 weeks prep time) Trophozoites and Schizonts P. berghei and yoelii sporozoites (4 weeks prep time) Drug Sensitivity Assays in vitro antimalarial assays (P. falciparum) in vivo antimalarial assays (rodent model) Gametocytes Training Malaria culture techniques Membrane feeding assay Safe technique for infected mosquito handling Sporozoites
Parasite Core Facility Custom Services and Collaboration Opportunities • Generation of Transgenic parasite lines • Transmission blocking assays • IFA (Mosquito stages) Antimalarial Assays • Blood schizontocidal assay • P. falciparum gametocytocidal assays Viaflo Liquid handling system • In vivo drug efficacy assays
Genomic Analysis and Sequencing Core Facility Facility Director: Andrew Pekosz, PhD Email: apekosz1@jhu.edu Facility Manager: Anne Jedlicka, MS Email: ajedlic1@jhu.edu Amanda Dziedzic Email: adziedz1@jhu.edu Genomic Analysis and Sequencing Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room E4208 Baltimore, Maryland 21205 Telephone: 443-287-5967 fax: 410-955-0105
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