S ynthetic U nits for R edirecting F unctionalities
Nature is a great source of inspiration for innovation and application
Flagella: Conserved function but high variability
Allowing more flexibility: SilverSURF B. subtilis S. typhimurium S. cerevisiae
Domain insertion leads to altered flagellum morphology
Can we use the scaffolding potential of the flagellar filament for other purposes? DARPin Ec Ec1
We wanted to investigate the effect of inserting a DARPin into the Flagellin
We had to think of an alternative… Streptavidin DARPin Ec1 StrepDARPidin Improve a brick: iGEM-Team Harvard 2006 Strep-tagged Filament
StrepDARPidin binds to EpCAM + cancer cells EpCAM + EpCAM EpCAM + EpCAM -
A binding assay confirms the interaction between Strep-tagged Flagellin and StrepDARPidin St Stre rep-tagged Flagellin StrepDARPidin Flagellin & St Stre repDARPidin StrepDARPidin Strep-tagged Flagellin StrepDARPidin Strep-tagged Flagellin
Our system could be used for different medical applications in the future Induce Apoptosis Fluorescent marking Immunesystem activation
Redirecting the whole protein assembly machinery Grand prize 2013 Heidelberg tRNA binding Amino acid activating domain Arc1p-C domain PheA Improve a brick: Heidelberg 2013
The molecular model enables us to predict well-working fusion proteins Special thanks to our advisor Florian Altegoer for helping us with the crystallization
To test our model we synthesized the fusion proteins Match of model and reality
An example of a possible new application: Marking of proteins
What is the use of the best application without safety?
What happens if a mutation occurs? Constitutive promoter
The worst case: Let us assume we lose the essential gene.
Time course of YvyD concentration
Mutation safe by three layers of security
The Big Picture RiboSURF CancerSURF SURFkiller SilverSURF
SynAesthetic Biology: Science without Sight
True equals visualisable?
Cooperation the mission: capacity building
Preparation
BLISTA
How do you show someone who can not see, the things that you can not see yourself? (and where you rely on visualization techniques)
Do we really know what we see – or do we see what we know?
Attributions AG Marahiel AG Bange AG Bölker AG Maierl AG Steinhoff AG Graumann AG Voigt AG Waldminghaus AG Grosse AG Hoyer Dr. Gundula Meißner, Bettina Happel, Prof. Dr. Christian Wagner, Dr. V. Krey (TUMunich), Alex Schmidt and Markus Esswein BLISTA Marburg Instructors: Prof. Dr. Michael Bölker and Dr. Gert Bange Advisor: Dr. Georg Fritz, Daniel Schindler, Felix Dempwolf and Florian Altegoer
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