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MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael - PowerPoint PPT Presentation

MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael Borowitzka S&C1.48 Limits to Growth Nutrients N (as nitrate, ammonia, urea) P Fe Si (diatoms) Supply of inorganic C (CO 2 /HCO 3 - )


  1. MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael Borowitzka S&C1.48

  2. Limits to Growth • Nutrients – N (as nitrate, ammonia, urea) – P – Fe – Si (diatoms) • Supply of inorganic C (CO 2 /HCO 3 - ) • Mixing (turbulence & shear) • Light (except for heterotrophic culture) • Temperature

  3. Flask Cultures

  4. Flask Cultures

  5. Carbuoy

  6. Tubular Airlift Reactor

  7. Commercial-Scale production Systems

  8. Chlorella production - Taiwan

  9. Chlorella production - Indonesia

  10. Rores - Kolar, Karnataka, India

  11. Spirulina Production Ponds, India

  12. Earthrise Spirulina plant – Calipatria, California Curtesy – Ahma Belay

  13. NatureBeta Dunalialla plant, Eilat, Israel curtesy – Ami Ben Amotz

  14. Ash-Free DW Lipid CaCO 3 Pleurochrysis carterae productivity – Perth, WA

  15. Hutt Lagoon; WA

  16. Whyalla; SA

  17. Dunaliella & β -Carotene • β -Carotene formation depends on (a) total irradiance and (b) salinity • Optimum salinity for growth ~ 22% NaCl • Optimum salinity for β -Carotene formation > 30% NaCl • Nutrient limitation

  18. Tank Culture - Hawaii

  19. Cascade System – Trebon, Czech Repiblic

  20. Covered Ponds USA

  21. Bag culture in Aquaculture Hatchery Bag Culture -

  22. Haematococcus ‘tube’ culture in Israel Bag Culture - Israel curtesy – Ephraim Cohen

  23. 1000 L pilot-scale BIOCOIL at Murdoch University

  24. Pilot Biocoil – Luton, UK

  25. Comercial Haematococcus plant: Israel

  26. Photobioreactor Tubular China

  27. Pilot CO 2 bioremediation plant operated by Greenfuel Technologies Corporation, USA at MIT.

  28. Haematococcus plant operated by Algatech Ltd, Kibbuz Ketura, Israel

  29. Alveolar panels

  30. Capital Running Cell Relia- Cost Cost Yield bility Shallow Ponds * (1) ** (2) * * Raceways *** (2) *** ** ** Cascade System *** (2) **** ** **** Tubular Photobioreactor ****** **** *** **** Fermenter ***** (3) ******* ****** ***** 1 Depends on land & water cost as very large pond area required; 2 The range of species which can be cultured is very limited; 3 Heterotrophic culture is VERY much cheaper as no light required.

  31. Algal Physiology, Bioreactor Design, Biochemistry & Engineering & Molecular Process Biology Control Light supply, Gas Photosynthetic efficiency, exchange, Turbulence, Secondary metabolism, Temperature control, ‘Stress’ tolerance, Materials, Harvesting, Strain improvement Downstream processing New applications, Economics marketing, formulation, packaging

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