MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael Borowitzka S&C1.48
Limits to Growth • Nutrients – N (as nitrate, ammonia, urea) – P – Fe – Si (diatoms) • Supply of inorganic C (CO 2 /HCO 3 - ) • Mixing (turbulence & shear) • Light (except for heterotrophic culture) • Temperature
Flask Cultures
Flask Cultures
Carbuoy
Tubular Airlift Reactor
Commercial-Scale production Systems
Chlorella production - Taiwan
Chlorella production - Indonesia
Rores - Kolar, Karnataka, India
Spirulina Production Ponds, India
Earthrise Spirulina plant – Calipatria, California Curtesy – Ahma Belay
NatureBeta Dunalialla plant, Eilat, Israel curtesy – Ami Ben Amotz
Ash-Free DW Lipid CaCO 3 Pleurochrysis carterae productivity – Perth, WA
Hutt Lagoon; WA
Whyalla; SA
Dunaliella & β -Carotene • β -Carotene formation depends on (a) total irradiance and (b) salinity • Optimum salinity for growth ~ 22% NaCl • Optimum salinity for β -Carotene formation > 30% NaCl • Nutrient limitation
Tank Culture - Hawaii
Cascade System – Trebon, Czech Repiblic
Covered Ponds USA
Bag culture in Aquaculture Hatchery Bag Culture -
Haematococcus ‘tube’ culture in Israel Bag Culture - Israel curtesy – Ephraim Cohen
1000 L pilot-scale BIOCOIL at Murdoch University
Pilot Biocoil – Luton, UK
Comercial Haematococcus plant: Israel
Photobioreactor Tubular China
Pilot CO 2 bioremediation plant operated by Greenfuel Technologies Corporation, USA at MIT.
Haematococcus plant operated by Algatech Ltd, Kibbuz Ketura, Israel
Alveolar panels
Capital Running Cell Relia- Cost Cost Yield bility Shallow Ponds * (1) ** (2) * * Raceways *** (2) *** ** ** Cascade System *** (2) **** ** **** Tubular Photobioreactor ****** **** *** **** Fermenter ***** (3) ******* ****** ***** 1 Depends on land & water cost as very large pond area required; 2 The range of species which can be cultured is very limited; 3 Heterotrophic culture is VERY much cheaper as no light required.
Algal Physiology, Bioreactor Design, Biochemistry & Engineering & Molecular Process Biology Control Light supply, Gas Photosynthetic efficiency, exchange, Turbulence, Secondary metabolism, Temperature control, ‘Stress’ tolerance, Materials, Harvesting, Strain improvement Downstream processing New applications, Economics marketing, formulation, packaging
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