1433/06/28 Reactor Design 1 2 1 1433/06/28 Algae Microalgae - PDF document

1433/06/28 Reactor Design 1 2 1

1433/06/28 Algae Microalgae Macroalgae Algae cultivation can be achieved in two ways: Photobioreactors Open ponds (PBR) 3 Easier to construct and operate Open ponds than most closed systems Contamination by other fast growing heterotrophs have restricted the commercial production of algae in open culture systems to only those organisms that can grow under extreme conditions. Their mass transfer rates are very poor resulting to low major limitations in open biomass productivity. ponds include poor light utilization by the cells, evaporative losses, diffusion of CO 2 to the atmosphere, and requirement of large areas of land. 4 2

1433/06/28  Optimal open pond dimensions are:50m*5m*0.4m with 100 tons of water media.  Maximum spirulina productivity is 0.35 g/lit.  100 tons capacity pond is 35kg/day  A paddle wheel consists of three structural units: paddle blades, motor and gear box. A paddle blade is made of aluminium, stainless steel or fiber glass, based on the requirements. 5  Fiber glass paddle wheels are preferred for algae cultivation in salt water ponds. The width and depth of the paddle wheel and the speed of the paddles going around are customized based on the pond specifications. The Spirulina Paddle Wheel short diameter (30 cm), high rpm 6 3

1433/06/28 A photobioreactor is a closed equipment which provides acontrolled environment and enables high productivity of algae.. PBRs facilitate better control of culture environment such as carbon dioxide supply, water supply, optimal temperature, efficient exposure to light,, gas supply rate, mixing regime, etc. 7 Cultivation of algae is in controlled circumstances, hence potential for much higher productivity Large surface-to-volume ratio. PBRs offer maximum efficiency in using light and therefore greatly improve productivity. Typically the culture density of algae produced is 10 to 20 times greater than bag culture in which algaeculture is done in bags - and can be even greater. Reduction in evaporation of growth medium. 8 4

1433/06/28 More uniform temperature. Better protection from outside contamination. Space saving - Can be mounted vertically, horizontally or at an angle, indoors or outdoors. 9 Disadvantages of Photobioreactors Capital cost is very high. Despite higher biomass concentration and better control of culture parameters, data accumulated in the last two decades have shown that the productivity and production cost in some enclosed photobioreactor systems are not much better than those achievable in open-pond cultures. The technical difficulty in sterilizing these photobioreactors has hindered their application for algae culture for specific end-products such as high value pharmaceutical products 10 5

1433/06/28 parameter Reletive Contamination risk Ponds > PBRs Space required Ponds ~ PBRs Productivity Ponds ~ PBRs Water losses Ponds > PBRs CO2 losses Ponds > PBRs O2 Inhibition Ponds < PBRs Process Control Ponds ~ PBRs Biomass Concentration Ponds < PBRs Capital/Operating Costs Ponds << PBRs 11 Tubular photobioreactors Flat-plate Photobioreactors Types of PBR Bubble column Photobioreactors 12 6

1433/06/28 Tubular photobioreactor is one of the most suitable types for outdoor mass cultures Most outdoor tubular photobioreactors are usually constructed with either glass or plastic tube and their cultures are re- circulated either with pump or preferably with airlift system. They can be in form of horizontal / serpentine, vertical near horizontal, conical, inclined photobioreactor 13 Tubular photobioreactor are very suitable for outdoor mass cultures of algae since they have large illumination surface area. Max 30cm radius due to light penetration problems Motor_pump mixing Maximum spirulina productivity:0.8 g/lit 100 tons capacity tubular system=80kg/day 1200m2 area required for 100 tons of water media One of the major limitations of tubular photobioreactor : some studies have shown that very high dissolved oxygen (DO) levels are easily reached in tubular photobioreactors . 14 7

1433/06/28 Photoinhibition is very common in outdoor tubular photobioreactors It is difficult to control culture temperatures in most tubular photobioreactors. Although they can be equipped with thermostat to maintain the desired culture temperature, this could be very expensive and difficult to implement. 15 Prospects Large illumination surface area, suitable for outdoor cultures, fairly good biomass productivities, relatively cheap. Limitations Gradients of pH, dissolved oxygen and CO 2 along the tubes. 16 8

1433/06/28 flat-plate photobioreactors  Flat-plate photobioreactors have received much attention for cultivation of photosynthetic microorganisms due to their large illumination surface area. The work presented by Milner (1953) paved way to the use of flat culture vessels for cultivation of algae. 17 18 9

1433/06/28 Prospects  Large illumination surface area  Good biomass productivities  Easy to clean up  Low oxygen buildup. Limitations Difficulty in controlling culture temperature Possibility of hydrodynamic stress to some algal strains 19 Bubble column reactors are cylindrical vessel with height greater than twice the diameter. Lower capital and operating cost in vertical column (without moving parts). To decrease contamination and cell damage due to shear. Relatively homogenous culture environment. High surface area to volume ratio. lack of moving parts. 20 10

1433/06/28  Photobioreactor design and operation are discussed in terms of mixing, carbon utilization, and the accumulation of photosynthetically produced oxygen. 21 Light energy supply CO 2 enrichment Oxygen Remoal Medium supply Mixing Temperature, pH, etc 22 11

1433/06/28 When selecting a light source , both spectral quality and intensity must be considered. Maximum specific growth rate Photoinhibition region Light saturation constant Sun light intensity 23 Light must not be too strong nor too weak. In most algal- cultivation systems, light only penetrates the top 3 inches (7.6 cm) to 4 inches (10 cm) of the water. This is because as the algae grow and multiply, they become so dense that they block light from reaching deeper into the pond or tank • The spectral quality of light utilized by algae is defined by the absorption spectrum in the range of 400 to 700 nm for the chlorophylls and other photosynthetically active pigments 24 12

1433/06/28 Light The major factor in algal growth and productivity  A schematic representation of light-response curves, i.e. the dependency of photosynthesis vs irradiance. The initial slope of the curve ( α ) is the maximum light utilisation efficiency. When the photoinhibition occurs (for high irradiancies), also an optimal value for irradiance Iopt can be detected. 25 How light affects productivity  Availability and intensity of light are the major factors controlling productivity of photosynthetic cultures In continuous culture S.S D = µ P=µ C b p =DC b  Generally µ increases with increasing irradiance, reaching a maximum value µmax . Further increase in irradiance may actually inhibit growth — a phenomenon known as photoinhibition. 26 13

1433/06/28 Monod relation 27 Models for light – dependent specific growth rate Equation Reference Tamiya et al. (1953) Van Oorschot (1955) Steele (1977) Bannister (1979) 28 14

1433/06/28 Kinetic parameters (Eq. (8)) for an outdoor culture of P. tricornutum (Acie ´ n Ferna ´ ndez et al., 1998) parameter value µm .063 Ik 94.3 Ki 3426 a 3.04 b 1.209 c 514.6 29 Carbon dioxide (or bicarbonate after it dissolves into the culture medium) is the source of the carbon in photoautotrophically growing cells. The upper and lower limits of CO 2 are not well define, but aeration of algal cultures with 5~15% CO 2 (or even pure CO 2 ) is fairly routine laboratory practice. The required inorganic carbon is most commonly introduced in the form of bubbles of enriched CO 2 gas mixture. 30 15

1433/06/28  Oxygen is product of photosynthesis, but a high level of dissolved oxygen inhibits algal growth, even at high concentrations of CO 2 .  The removal of excess O 2 is a mass transfer problem comparable to that of the CO 2 supply. At present, two major solution of, increasing turbulence, and O 2 stripping with air, are open to the photobioreactor design and operation. 31  Nutrient availability is an important factor controlling the levels of the primary productivity of photosynthetic organisms.  Media with different compositions are supplied to the cells in the form of carbon dioxide, water and mineral salts in macro or micro quantities.  The macronutrients include carbon, nitrogen, phosphorus, hydrogen, oxygen, sulfur, calcium, magnesium, sodium potassium and chlorine.  The micronutrients needed in trace quantities of micro-, nano- or even picograms per liter are iron, boron, manganese, copper, molybdenum, vanadium, cobalt, nickel, silicon and selenium.  The optimum N: P ratio for phytoplankton growth generally is about 15: 1 32 16