Laboratory for Swine Diseases and the Danish disease surveillance program Birgitta Svensmark, DVM, PhD Head of Laboratory, SEGES Danish Agriculture and Food Council
Pig Research Center for Pig Production Danish Agriculture and Food Council 2...| 23. maj 2015
SURVEILLANCE PROGRAM IN DENMARK ● Surveillance for exotic diseases ● SPF- program ● National salmonella surveillance program 3...| 23. maj 2015
• 5 millions people • 30 millions pigs annually • Family ownen farms • 4000 pig farms • One national wide cooperation of farmers • Export of 10 millions live pigs annually • 85% of the pork exported globally 4...| 23. maj 2015
SPECIFIC PATHOGEN FREE: ● 3.116 herds have SPF-status ● 75% of the sows in Denmark are SPF sows 5...| 23. maj 2015
THE DANISH SPF SYSTEM ● Established in 1971 ● Specific Pathogen Free herds ● Very ambiciuos plans from the start ● They included the SPF-diseases, we have today ● - and a lot more that could not be controlled 6...| 23. maj 2015
THE DANISH SPF SYSTEM Start ● Enzootic pneumoniae ● Actinobacillus pleuropneumoniae, type 2 ● Atrofic rhinitis ● Swine dysentery ● Mange ● Lice ● Haemophilus parasuis ● Erysipelothrix rhusiopathiae ● Cl. perfringens, type C ● Aujeszkys Disease 7...| 23. maj 2015
THE DANISH SPF SYSTEM Next ● Enzootic pneumoniae ● Actinobacillus pleuropneumoniae, type 2 ● Atrofic rhinitis ● Swine dysentery ● Mange ● Lice ● Haemophilus parasuis ● Erysipelothrix rhusiopathiae ● Cl. perfringens, type C ● Aujeszkys Disease 8...| 23. maj 2015
THE DANISH SPF SYSTEM Next ● Enzootic pneumoniae ● Actinobacillus pleuropneumoniae, type 2 ● Atrofic rhinitis ● Swine dysentery ● Mange ● Lice ● Haemophilus parasuis ● Erysipelothrix rhusiopathiae ● Cl. perfringens, type C ● Aujeszkys Disease 9...| 23. maj 2015
SPECIFIC PATHOGEN FREE: CONTROL AND DECLARATION 2015 ● 7 infectious swine diseases: ● Enzootic pneumoniae ● Actinobacillus pleuropneumoniae, different types ● Atrofic rhinitis ● Swine dysentery ● Mange ● Lice ● PRRS Examples: SPF + Myc + Ap6 + PRRS EU 10...| 23. maj 2015
BREEDING PYRAMID = HEALTH PYRAMID Nucleus 260 8.000 Herds sows L Y D Clinical examination Blood test Multiplier once a month 58.000 sows L or Y Sow Herds 2.750 1.000.000 sows LY Herds Clinical examination Blood test Finishers once a year 19.500.000 pigs produced LYD 10.000.000 pigs exported (weaners, breeding stock) 11...| 23. maj 2015
SPF SAMPLING PROTOCOL RED HERDS • 12 times a year: • Mycoplasma hyopneumonia • APP serotypes 2 and 6 • PRRS EU and US • Salmonella – mandatory by law • Clinical signs for dysentery, atrofic rhinitis, mange and lice • 4 times a year: • APP serotype 12 • 2 times a year: • Atrofic Rhinitis by nasal svabs • APP serotype 5 • 1 time a year: • APP serotypes 1 (9), 7 (4), 10 No. 12 23.05.2015
SPF SAMPLING PROTOCOL BLUE HERDS • 1 times a year: • Mycoplasma hyopneumonia • APP serotypes 2, 6 and 12 • PRRS EU and US • Clinical signs for dysentery, atrofic rhinitis, mange and lice. No. 13 23.05.2015
VETERINARY DEPARTMENT 1971 Laboratory for Serology, Roskilde Myc. hyopneumoniae A. pleuropneumonia, type 2 and 6 14...| 23. maj 2015
VETERINARY DEPARTMENT 1971 1988 The Laboratory in Laboratory for Serology, Kjellerup Roskilde Myc. hyopneumoniae Pathology A. pleuropneumonia, Microbiology type 2 and 6 Parasitology 15...| 23. maj 2015
VETERINARY DEPARTMENT 1971 1988 1998 The Laboratory in The Laboratory in Laboratory for Serology, Kjellerup Kjellerup Roskilde Myc. hyopneumoniae Pathology Pathology A. pleuropneumonia, Microbiology Microbiology type 2 and 6 Parasitology Parasitology Salmonella surveillance 16...| 23. maj 2015
2002 17...| 23. maj 2015
LABORATORY FOR SWINE DISEASES 1971 2003 Laboratory for Swine Laboratory or serology, Diseases Roskilde Myc. hyopneumoniae Pathology A. pleuropneumonia, Microbiology type 2 and 6 Parasitology Salmonella surveillance Serology 18...| 23. maj 2015
LABORATORY FOR SWINE DISEASES ● 6,5 veterinarian ● 16 laboratory technicians ● 1 technician ● 1 secretary ● Accredited in DANAK since 2004 19...| 23. maj 2015
LABORATORY FOR SWINE DISEASES, 2015 ● Pathology Serology ● ● Microbiology Mycoplasma hyopneumoniae ● Antibiogram ● A. pleuropneumoniae, ● Parasitology type 2, 6 and 12 ● Salmonella cultivation ● PRRS, not typeable ● Control on semen ● Salmonella on serum ● Salmonella on meatjuice 20...| 23. maj 2015
QUALITY CONTROL ● Ring trials, national and international, 1- 6 times a year 21...| 23. maj 2015
2014 Pigs and organs 4.550 USK, stomachs, lungs, reproductive organs 15.495 Nasal swabs, atrofic rhinitis 4.170 Feces pools, salmonella 2.620 Serology, tests, serum 266.900 Meat juice, salmonella serology 255.500 22...| 23. maj 2015
WHY ? 23...| 23. maj 2015
WHY ? Diagnostic Disease Bad performance Legislation 24...| 23. maj 2015
WHY ? Diagnostic Exotic diseases Disease Bad performance Legislation 25...| 23. maj 2015
WHY ? Surveillance Diagnostic Exotic diseases Disease Bad performance National Salmonella SPF Surveillance Program Legislation 26...| 23. maj 2015
WHY ? Surveillance Diagnostic Exotic diseases Disease Bad performance National Salmonella SPF Surveillance Program Legislation Dysentery, Atrofic Mange Clinical cases rhinitis Serology 27...| 23. maj 2015
SEROLOGICAL TEST IN 2014 AT LABORATORY FOR SWINE DISEASES 2014, total Red herds Other herds Total Myc. hyopneumoniae 33.409 8.513 45.667 Ap2 46.115 16.469 68.320 Ap6 40.044 12.307 57.927 Ap12 17.703 6.686 24.916 PRRS 26.486 19.705 50.186 Salmonella 18.663 1.219 19.882 182.420 64.899 266.898 nr 28 23.05.2015
WHY ? Surveillance Diagnostic Exotic diseases Disease Bad performance National Salmonella SPF Surveillance Program Legislation Dysentery, Atrofic Mange Clinical cases rhinitis Serology 29...| 23. maj 2015
WHY ? Surveillance Diagnostic Exotic diseases Disease Bad performance National Salmonella SPF Surveillance Program Legislation Serum in breeding Clinical cases Feces Meat juice and multiplying herds Dysentery, Atrofic Mange Clinical cases rhinitis Serology 30...| 23. maj 2015
NATIONAL SALMONELLA SURVEILLANCE PROGRAM ● Breeding and multiplying herds are tested serologically every month by 10 blood samples. ● A Salmonella index is calculated based on the last 3 months. ● If the index is more than 5, the herd has to investigate feces samples for salmonella. ● Furthermore they have to pay a fee for each sold pig. 31...| 23. maj 2015
NATIONAL SALMONELLA SURVEILLANCE PROGRAM ● Every herd, that is slaugthering more than 400 pigs per year, has to be tested for antibodies for Salmonella by meat juice. 32...| 23. maj 2015
33...| 23. maj 2015
34...| 23. maj 2015
NATIONAL SALMONELLA SURVEILLANCE PROGRAM ● The results are classified as positive if OD% ≥ 20 and negative if the OD % < 20 ● The herds are classified in 3 levels depending of the results based on the last 3 months. ● The level 2 and 3 has to report the supplier of weaners. This herd has to investigate feces samples. 35...| 23. maj 2015
NATIONAL SALMONELLA SURVEILLANCE PROGRAM ● The last surveillance for the Salmonella is the investigation of feces samples. ● Breeding and multiplying herds with high salmonella index and sow herd who has delivered pigs to a slaugther herd in level 2 or 3 has to examine feces samples for salmonella. 36...| 23. maj 2015
WHY ? Surveillance Diagnostic Exotic diseases Disease Bad performance National Salmonella SPF Surveillance Program Legislation Serum in breeding Clinical cases Feces Meat juice and multiplying herds Dysentery, Atrofic Mange Clinical cases rhinitis Serology 37...| 23. maj 2015
CLINICAL SALMONELLOSIS
CLINICAL SALMONELLOSIS 39...| 23. maj 2015
CLINICAL SALMONELLOSIS 40...| 23. maj 2015
Sometimes you have to search for the solution. 41...| 23. maj 2015
Serological examinations for APP serotypes, Denmark 2013 ApP serotype No. of samples No. of pos 1 4360 0 2 73461 1052 (14%) 3 0 0 Not recorded 4 0 0 Not recorded 5 2237 9 (4%) 6 61187 941 (15%) 7 4421 13 (5%) 8 20 0 9 11 0 10 4471 10 (2%) 11 11 0 12 23242 482 (2%) Cross reactions: 1,9,11 and 3,6,8 and 4,7 in LPS 23.05.2015 No. 42
APP BACTERIA TYPES 2013 ● No. of isolates typed : 176 Type No. Pos isolates 2 108 5 2 6 40 7 1 8 2 12 12 No. 43 23.05.2015
RED HERDS APRIL 2014 THE SPF DISEASES NEGATIVE HERDS (%) PRRS EU STRAIN 92 PRRS US STRAIN 99 Test method: Ideex antibody test EU and US No. 44 23.05.2015
23.05.2015 No. 45
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