Kingston, Canada Queens Canada iGEM 2011 World Championship - PowerPoint PPT Presentation


 Kingston, Canada Queens Canada iGEM 2011 World Championship Jamboree content the problem The Challenge
 Approach & Methods
 Our Solution
 Future Applications
 Accomplishments


why C. elegans ? - Excellent chemotaxis mechanism - Multi-cellular= multiple processes - Hardy organism microinjection conventional method of BioBrick assembly 1C3 1A3 1T3

kill switch mechanism kill switch mechanism Target(Gene(

future applications - Characterize(in( C.)elegans (( - Assembly(with(other(enzymes(in( C.)elegans ,( create(full(pathway( - BioBrick(Registry:(develop(bioremedia8on( pathways(for(other(chassis((( ( does it work? transparent fast rate of reproduction

part improvement applications - Modify(exis8ng(eCFP(part( - Test(new(promoters(effec8vely(( Tailings(Pond(

chemotactic assay chemotaxis part design- promoter - Conducted(preferen8al(assay(between(target(( and(control(substances( GPCR' Source' Ligand' ADBR2( Mouse( Phenol( Orexin( Human( Toluidine,(4EChlorobenzoic(acid,(( Naphthalene(( OX1R( Mouse( 4EChlorobenzoic(acid( !"#$% = # ! !"!#$ ! !"#$% ! !" ! !"#$%& ! !"#$%#&' ! − # ! !"!#$ ! !"#$% ! !" ! !"#$%"& ! !"#$%#&' ! Foreign(GPCR( MARM1( Rat( Phenol( ! # ! !"!#$ ! !"#$% NPYR1( Mouse( Naphthalene( ! ( results results Part: ODR-7: ADRB2-:UNC-54 (BBa_K631032)

accomplishments sponsors ! Successfully(expressed(eCFP(in(a(nematode( (( ! Developed(a(novel(method(of(assembly,(PCR( Liga8on( ! Maintained(balance(in(our(approach(to(the( science(by(considering(human(prac8ces(( ( acknowledgements references Faculty Advisors : Dr. Chin-Sang, Dr. Greer, Dr. Ko, Dr. Bendena, Dr. 1.(Ahmed(S,(Hodgkin(J.((2000)(MRTE2(checkpoint(protein(is(required(for(germline(immortality(and(telomere(replica8on(in( C.)elegans .(Nature,(403:159E164(( ( Ramsay, & Dr. Young. 2.(Smelick(C,(Ahmed(S.((2005)(Achieving(immortality(in(the( C.)elegans (germline.(Ageing(Research(Reviews,(4(1):67E82(( ( 3.(Telomere(Shortening(and(Damage((2009)((hQp://www.immortalhumans.com/telomereEshorteningEandEdamage/)( ( 4.(Fire(A,(Xu(S,(Montgomery(MK,(Kostas(SA,(Driver(SE,(Mello(CC.((1998)(Potent(and(specific(gene8c(interference(by(doubleEstranded(RNA(in(Caenorhabdi8s( Lab Support : Tony Papanicolaou, Jeff Boudreau and rest of Chin-Sang elegans.(Nature,(391(6669):806E11( ( lab. 5.(Tabara(H,(Grishok(A,(Mello(CC.((1998)(RNAi(in( C.)elegans :(soaking(in(the(genome(sequence.(Science,(282(5388):430E1( ( 6.(Timmons(L,(Fire(A.((1998)(Specific(interference(by(ingested(dsRNA.(Nature,(395(6705):854( ( 7.(Timmons(L.((2006)(Construc8on(of(Plasmids(for(RNA(Interference(and(In(Vitro(Transcrip8on(of(DoubleEStranded(RNA.(Methods(Mol(Biol.(351:109E17.( Past QGEM Team Members : Steve Goldie, Chris Palmer, Geoff ( 8.(Simmer(F,(Tijsterman(M,(Parrish(S,(Koushika(SP,(Nonet(ML,(Fire(A,(Ahringer(J,(Plasterk(RHA.((2002)(Loss(of(the(Puta8ve(RNAEDirected(RNA(Polymerase( Halliday, Harry Zhou and James MacLeod. RRFE3(Makes(C.(elegans(Hypersensi8ve(to(RNAi.(Current(Biology,(12(15):(1317E1319.( ( 9.(Kiyohara(H,(Torigoe(S,(Kaida(N,(Asaki(T,(Iida,(T,(Hayashi(H(and(Takizawa(N.((1994)(Cloning(and(Characteriza8on(of(a(Chromosomal(Gene(Cluster,(pah,(That( Encodes(the(Upper(Pathway(for(Phenanthrene(and(Naphthalene(U8liza8on(by(Pseudomonas(pu8da(OUS82.(Journal(of(Bacteriology,(176:2439E2443.(( QGEM 2011 : Tony He, Stephanie Zhou, Olivia Margie, Alex Campbell, ( Graphics' (order(of(appearance)( Allister Smith, David Dai, Sophie Gong, Kevin Chen, Kristina Collavino, hQp://s.ngm.com/2009/03/canadianEoilEsands/img/candianEoilEsandsE615.jpg(( Eni Rukaj, Faisal Bakhteyar, Eszter Gereb, Gary Wu, Wei Liu, Anujan hQp://en.wikipedia.org/wiki/GPCR(( hQp://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf( Poologaindran. hQp://www.ncbi.nlm.nih.gov/pmc/ar8cles/PMC205370/((( hQp://wwwEfgg.eur.nl/ch1/cellbiology/images/jansen1.jpg(( QGEM 2010/2011 Executive Team