Giant Jamboree Boston 10/31/14 Pollution Environmental Health - PowerPoint PPT Presentation

Giant Jamboree Boston 10/31/14

Pollution Environmental Health Problems (Reference: The Drive Behind Mission #1 Plastic Pollution. Retrieved from http://www.everythingconnects.org/plastic-pollution.html Web site of digit8 design. Retrieved from http://gogogloglo.wordpress.com/2010/11/03/national-geographic-water-the-source-of-life/ Website of National Geographic. Retrieved from http://education.nationalgeographic.com/education/encyclopedia/great-pacific- garbage-patch/?ar_a=1 )

Degredo PETronum !

Plastic PET Production Association of Postconsumer Plastics Recyclers (APR)

Plastic PET Production • 265 Mtone produced each year Association of Postconsumer Plastics Recyclers (APR)

PLASTIC PRODUCTION / PLASTIC RECYCLING HYPERTENSION DIAGNOSTICS INC

Disposal, Recycling and Energy Recovery 2012 Pyruvate

Why not synthetic?

PET Chemical Structure PET consists polymerized units of the monomer ethylene terephthalate, with repeating C 10 H 8 O 4 units (Reference: Polyethylene terephthalate. Wikipedia. Retrieved from http://en.wikipedia.org/wiki/Polyethylene_terephthalate )

2010 2012 London Imperial UC_Davis College PET 2012 2014 METU iGEM TU Darmstadt

Kanahisa Laboratories

Kanahisa Laboratories

Structural Analysis Blanka Vrchotová, Martina Macková, Tomáš Macek and Kate ř ina Demnerová (2013). Bioremediation of Chlorobenzoic Acids, Applied Bioremediation - Active and Passive Approaches, Dr. Yogesh Patil (Ed.), ISBN: 978-953-51-1200-6, InTech, DOI: 10.5772/56394. Available from: http://www.intechopen.com/books/applied- bioremediation-active-and-passive-approaches/bioremediation-of-chlorobenzoic-acids

PET- Pyruvate Pathways http://2014.igem.org/Team:METU_Turkey_project

Our Circuit Design J23108 K316003 K1095000 K1392931 K1392932 B1006 C.Promoter RBS+Catechol Hydrolase Hydratase Aldolase Terminator 2,3-dioxygenase

New part Bba_K1392931 2-oxopent-4-enoate + H2O  4-hydroxy-2-oxopentanoate 2-oxopent-4-enoate hydratase Escherichia Coli BL21(DE3)

New part Bba_K1392932 4-hydroxy-2-oxoglutarate  pyruvate + glyoxylate 4-hydroxy-2-oxoglutarate aldolase Escherichia Coli BL21(DE3)

Characterization of Catechol-2,3- dioxygenase K1392991 = J23108 + K316003

Kill-Switch K80800 K515004 C0040 B1006 AraC RBS+Antiholin Tet+LVA Terminator R0040 K112806 K124014 B1006 TetR T4 Endolysin Holin Terminator

The issue Arabinose present Arabinose absent

MODELING

MODELING Catechol Catechol 2-3 2-hydroxymuconate 2-hydroxymuconate dioxygenase semialdehyte semialdehyte hydrolase 4-hydroxy 2-oxopent 4-enoate 2-oxopent 2-oxopentaonate hydratase 4-enoate 4-hydroxy 2-oxovalerate aldolase Pyruvate

Catechol Degredation • Simulation of catechol diffuses by cell and degrades to pyruvate instantly http://2014.igem.org/Team:METU_Turkey_modeling

Parameters of Catechol Degredation Parameters of our simulation which we used in catechol degradation to pyruvate http://parts.igem.org/wiki/index.php?title=Part:BBa_K1392991

Characterization Characterization of our Bba_K1392991 part by exposing catechol Adding K1392991

Graphs of Kill Switch Model 1)Presence of arabinose the simulation of AraC promoter without holin and endolysin http://2014.igem.org/Team:METU_Turkey_modeling

Graphs of Kill Switch Model 2) Absence of Arabinose, the simulation of cell lysis by holin and endolysin http://2014.igem.org/Team:METU_Turkey_modeling

Parameters of Kill Switch Model Parameters of our kill switch model bu using AraC and TetR promoter http://2011.igem.org/Team:St_Andrews/modelling http://2011.igem.org/Team:ETH_Zurich/Modeling/Parameters

Achievements 2 New Parts added to the libary • K1392931 • K1392932 Composite and Characterization • K1392991 Composite Parts added to the libary • K1392971 • K1392972 • K1392939 • K1392938

InterLab Study

Third Device Second Device First Device 1) BBa_I20260 in the pSB3K3 vector 2) BBa_J23101 + BBa_E0240 in pSB1C3 backbone 3) BBa_J23115 + BBa_E0240 in pSB1C3 backbone

POLICY & PRACTICE

SynBio Survey!

II. METU The Day of Synthetic Biology!

PET Degradation Game

METU Development Foundation Schools • We gave Lab education to young iGEM'ers! • We have started, advised and helped to this year's High School Team "METUHS-Ankara"! • We are raising new iGEM'ers!

PET Degredation Meeting in Eski ş ehir!

Turkey iGEM Teams Meeting 08/06/14 Ankara,Turkey

SynBio Party! #2 SyBio Party! #3

Future Prospect Genetic Modified Organism

Thank you for your attention Special thanks to : Prof. Dr. Mahinur S. Akkaya Assoc. Prof Dr. Ça ğ da ş D. Son Assoc. Prof Dr. Mayda Gürsel R.A Ayça Çırçır R.A Çi ğ dem Yılmaz R.A Handan Melike Dönerta ş R.A Side Selin Su Yirmibe ş o ğ lu R.A Ali ş an Kayabölen CEO Burak Yılmaz

Sponsors

Team Visit our wiki !

Special thanks to : Prof. Dr. Mahinur S. Akkaya Assoc. Prof Dr. Ça ğ da ş D. Son Assoc. Prof Dr. Mayda Gürsel R.A Ayça Çırçır R.A Çi ğ dem Yılmaz Handan Melike Dönerta ş Side Selin Su Yirmibe ş o ğ lu Ali ş an Kayabölen CEO Burak Yılmaz