Missouri Miners 2014 Giant Jamboree Burning fossil fuels releases - PowerPoint PPT Presentation

Missouri Miners 2014 Giant Jamboree

 Burning fossil fuels releases flue gases  Contain many pollutants  Microbes can utilize

http://www.nma.org/index.php/coal-statistics

 Impact of coal emissions per year in the U.S. (Schneider and Banks, 2010) ◦ 13,000 ,000 premature deaths ◦ 20 20,00 ,000 heart attacks ◦ 1.6 6 million llion lost workdays  Pollutants need to be filtered effectively and economically Missouri S&T Coal Plant

 Lots of specialized equipment  Each pollutant has a different method of removal  Proper disposal of byproducts http://i.huffpost.com/gen/1380970/thumbs/o-COAL- POWER-PLANT-facebook.jpg

 Create and identify preexisting BioBricks  Express parts in photosynthetic organism  Incorporate organism into bioreactor NO NO x

http://www.ecs.umass.edu/che/henson_group/research/bioreactor.html

N 2 O

 E. coli K12 ◦ norV – oxidoreductase ◦ Hmp – nitric oxide deoxygenase ◦ nrfA – nitrite reductase  P. aeruginosa PAO1 ◦ norCB – nitric oxide reductase ◦ nosZ – nitrous oxide reductase

So Far Still in Progress  Bba_K113001 - nrfA  norV part  Bba_K896005 – norCB ◦ Internal PstI site removed ◦ Ligation trouble ◦ Bba_K896004 – nosZ and norCB  nosZ part together ◦ Amplification troubles  Bba_K1356006 – nosZ ◦ Internal site needs removal  Bba_K1 _K1370000 - hmp

 Further characterize hmp  Continue work on nosZ and norV  Functionalize genes  Test in bioreactor

 Student Design and Experiential Learning Center (SDELC)  Prestigious group of student- led, competitive design teams  We are the only biological engineering design team on our campus What’s coming up:  New lab space within the Center  Building corporate partnerships  Promot omoting ing the SDELC LC visi sion on of student dent succe ccess ss

 Exploring Synthetic Biology  Play with DNA! ◦ Residential Life: Midwest Regional Program of the Month Award  Speak Up Speak Out  Waynesville High School STEM Expo  Engineers’ Week at St. Louis Science Center  And many more!

 Cruz-Ramos, H., Crack, J., Wu, G., Hughes, M. N., Scott, C., Thomson, A. J., ... & Poole, R. K. (2002). NO sensing by FNR: regulation of the Escherichia coli NO- detoxifying flavohaemoglobin, Hmp. The EMBO journal, 21(13), 3235-3244.  Gardner, P. R., Gardner, A. M., Martin, L. A., & Salzman, A. L. (1998). Nitric oxide dioxygenase: an enzymic function for flavohemoglobin. Proceedings of the National Academy of Sciences, 95(18), 10378-10383.  Hausladen, A., Gow, A. J., & Stamler, J. S. (1998). Nitrosative stress: metabolic pathway involving the flavohemoglobin. Proceedings of the National Academy of Sciences, 95(24), 14100-14105.  Hmp fused nitric oxide dioxygenase/dihydropteridine reductase 2. (n.d.). Retrieved from http://www.ncbi.nlm.nih.gov/gene/947018  Schneider, C., and Jonathan Banks. 2010. The Toll From Coal: An Updated Assessment of Death and Disease from America’s Dirtiest Energy Source. Clean Air Task Force, September.

 Our advisors, Dr. Westenberg & Dr. Shannon  Missouri S&T SDELC  Missouri S&T Department of Biological Sciences  Missouri S&T Department of Chemistry  Missouri S&T Department of Chemical Engineering  Missouri S&T cDNA Center  Donor Fred Kielhorn  Former team members Alie Abele and Blythe Ferriere