Functionalization of PU foams Functionalization of PU foams via inor via inorganic and or ganic and organic coatings ganic coatings to improve cell and tissue interactions to impr ove cell and tissue interactions Titolo presentazione sottotitolo Serena Bertoldi 1,2 , Nicola Contessi Negrini 2 , Andrea Cochis 3,4 , Francesca Uberti 5 , Maria Cristina Tanzi 2 , Silvia Farè 1,2 1 Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Politecnico di Milano, Milano, Italy 2 INSTM, National Consortium of Materials Science and Technology, Local Unit Politecnico di Milano, Milano, Italy 3 Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy 4 Center for Translational Research on Autoimmune & Allergic Diseases – CAAD, Novara, Italy 5 Department of Translational Medicine, Università del Piemonte Orientale, Novara, Italy
Scaffolds pr Scaf folds production and r oduction and requir equirements ements • scaf scaffolds folds intended for tissue r issue regenerat egeneration ion must match specific r specific requir equirements ements • versat versatile formulat ile formulations ions to tune the structural pr structural propert operties ies 1
Scaffolds pr Scaf folds production and r oduction and requir equirements ements • scaf scaffolds folds intended for tissue r issue regenerat egeneration ion must match specific r specific requir equirements ements • versat versatile formulat ile formulations ions to tune the structural pr structural propert operties ies 1
Scaffolds pr Scaf folds production and r oduction and requir equirements ements • a corr a correct surface bioact ect surface bioactivity ivity is fundamental to properly guide cel guide cells fate ls fate and tissue issue infil infiltrat tration/r ion/regenerat egeneration ion different bioactive coatings can be used to promote a desired response http://www.composites.polito.it 2
Aim of the work Aim of the work • use of polyur polyuret ethane foams hane foams as scaffolding materials • coat coating ing of polyurethane foams either by using • calcium phosphates calcium phosphates à to target the regeneration of bone tissue • gelat gelatin hydr in hydrogels ogels à to target the regeneration of soft tissue 3
Pr Production of polyur oduction of polyurethane foams ethane foams • polyur polyuret ethane foams hane foams used as por porous structur ous structures es for scaf scaffolds pr folds product oduction ion • one-step gas foaming one-step gas foaming reaction • water water (2% w/w polyols ) used as expand expanding agent ing agent • versat versatile formulat ile formulations ions to tune the structural pr structural propert operties ies 4
Polyur Polyurethane foam coating to obtain hybrid scaf ethane foam coating to obtain hybrid scaffolds folds • PU PU foams foams coated by inor inorganic ganic or or organic ganic coating to target different tissues regeneration Inorganic Inor ganic coating coating Or Organic ganic coating coating 1) 1) PU PU samples 2) coating of the PU samples in an vacuum chamber 3) 3) inor inorganic ganic/or organic ganic coated samples Calcium Calcium Gelatin Gelatin PU_CaP PU_CaP PU_gel PU_gel phosphates phosphates hydr hydrogel ogel (6 or 15% (6 or 15% w/v) /v) 5
Morphology: Scanning Electr Morphology: Scanning Electron Micr on Microscopy oscopy PU_CaP PU_CaP PU PU Inorganic coating PU_gel PU_gel Organic coating 6
Morphology: Micro-CT Morphology: Micr o-CT PU PU PU_gel PU_gel • influence of influence of gelat gelatin in hydr hydrogels on por ogels on pore inter e interconnect connections ions 7
Morphology: Micr Morphology: Micro-CT o-CT 100 90 PU 80 PU_GEL6 70 PU_GEL15 open porosity [%] osity [%] 60 50 open por 40 30 20 10 0 dry hydrated • influence of gelat gelatin in hydrogels on pore interconnections • influence of hydrat hydration ion on por pore inter e interconnect connection ion 8
Water uptake: ater uptake: PU_gel PU_gel • Stabil Stability ity and water uptake water uptake at 37 °C 37 °C (physiological temperature) 600 500 Water uptake [%] 400 300 200 PU 100 PU_GEL6 PU_GEL15 0 0 100 200 300 400 500 600 700 Time [h] • PU foams PU foams: stable at 37 °C • Gelat Gelatin hydr in hydrogels ogels improve water uptake • PU_gel6 PU_gel6 higher water uptake compared to PU_gel15 PU_gel15 10
Water uptake: ater uptake: PU_CaP PU_CaP • Stabil Stability ity and water uptake water uptake at 37 °C 37 °C (physiological temperature) 400 350 ater uptake [%] Water uptake [%] 300 250 200 150 100 PU 50 PU_CaP 0 0 100 200 300 400 500 Time (h) Time (h) • PU foams PU foams: stable at 37 °C • CaPs CaPs coating: stable at 37°C 11
Mechanical compressive pr Mechanical compr essive properties: operties: PU_gel PU_gel 40 PU 35 PU_GEL6 PU_GEL15 30 Stress (kPa) 25 20 15 10 5 0 0 10 20 30 40 50 60 Strain (%) • stress/strain behavior PU_gel6 PU_gel6 and PU_gel15 PU_gel15 comparable to that of contr control PU ol PU • PU_GEL15 PU_GEL15: behavior slightly different from PU PU and PU_gel6 PU_gel6 12
Mechanical compr Mechanical compressive pr essive properties: operties: PU_gel PU_gel 70 PU PU_GEL6 60 PU_GEL15 50 40 [kPa] [kPa] 30 20 10 0 E (kPa) R10-30 (kPa) strain@max (kPa) • no significant difference between PU_gel6 PU_gel6 and PU_gel15 PU_gel15 versus PU PU scaffolds • low contribut low contribution ion of gelat gelatin coat in coating ing to mechanical properties 13
Mechanical compr Mechanical compressive pr essive properties: operties: PU_CaP PU_CaP 0.25 PU PU_CaP 0.2 ess [MPa] Stress [MPa] 0.15 0.1 Str 0.05 0 0% 10% 20% 30% 40% 50% 60% Strain [%] Strain [%] • PU_CaP PU_CaP: no improvement in the mechanical properties of the PU PU 14
Mechanical compr Mechanical compressive pr essive properties: operties: PU_CaP PU_CaP 350 PU 300 PU_CaP 250 200 [kPa] [kPa] 150 100 50 0 E (kPa) R10-30 (kPa) strain@max (kPa) • PU_CaP PU_CaP: values of the mechanical parameters à no significant difference compared to PU PU 15
In vitro cytotoxicity tests: In vitr o cytotoxicity tests: PU_gel PU_gel • Ind Indir irect cytotoxicity ect cytotoxicity on PU_ge PU_gel with ad adipose derived stem cel ipose derived stem cells ls • Target: soft t soft tissues r issues regenerat egeneration ion 120 day 1 day 3 day 7 100 Cell viability [% vs control] ol] Cell viability [% vs contr 80 60 40 20 0 PU PU_GEL6 PU_GEL15 • PU PU and PU_gel PU_gel showed no cytotoxic ef no cytotoxic effects fects 16
In vitr In vitro cytocompatibility tests: o cytocompatibility tests: PU_gel PU_gel • Dir Direct cytocompat ect cytocompatibil ibility ity on PU_GEL with ad adipose derived stem cel ipose derived stem cells ls • Target: soft t soft tissues r issues regenerat egeneration ion day 1 day 1 day 3 day 3 day 7 day 7 PU_GEL6 PU_GEL6 PU_GEL15 PU_GEL15 ! • Cel Cells ad ls adhesion hesion and lipid dr ipid droplets accumulat oplets accumulation ion à suitable for ad adipose t ipose tissue issue regenerat egeneration ion 17
In vitro cytocompatibility tests: In vitr o cytocompatibility tests: PU_CaP PU_CaP • Dir Direct cytocompat ect cytocompatibil ibility ity on PU_CaP with amnionic amnionic mesenchymal cel mesenchymal cells ls • Target: bone t bone tissue r issue regenerat egeneration ion PU PU PU_CaP PU_CaP • PU_CaP PU_CaP: cel cells ad ls adhesion hesion and inorganic matrix deposit deposition ion 18
Conclusions Conclusions • PU foam PU foam scaffolds à à successful successfully coated ly coated by inor inorganic ganic and or organic ganic coatings • possible possible use as scaf scaffolds folds for bone t bone tissue issue and soft t soft tissue issue regeneration • morphological analyses: morphological analyses: • ef effect fective coat ive coating ing by CaPs CaPs à à to mimic mimic the inor inorganic ganic bone bone component • gelat gelatin hydr in hydrogel ogel à à to impr improve cel ove cells ad ls adhesion hesion • adequate mechanical pr adequate mechanical propert operties ies for har hard and soft soft tissue r regenerat egeneration ion • cel cells successful ls successfully ad ly adher hered ed and pr prol oliferated iferated on the scaffolds à à corr correct funct ect functional ionality ity • depositing inor inorganic extracel ganic extracellular matrix lular matrix à à bone t bone tissue issue regeneration • accumulating lipid dr ipid droplets oplets à à ad adipose ipose tissue issue regeneration 19
Titolo presentazione THANK YOU sottotitolo
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