Finding Pneumo HKUST iGEM 2014 TEAM
In Intr troduc oduction tion Human n Introduc oducti tion on 2 Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor Pract ctice ce
Bac ackgr ground ound an and d Mo Motiv tive Otitis is Medi dia Pneumonia nia Meningi ngitis is Human n Introduc oducti tion on 3 Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor Pract ctice ce
Bac ackgr ground ound an and d Mo Motiv tive 40-70% 40 70% 1.6 mill llio ion n deaths hs per ye year ar Killed lled or Disabled abled Slovenia Human n Introduc oducti tion on 4 Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor Pract ctice ce
Current Methods X-ray PCR assay Require laboratory or hospital setting Sputum gram staining Urinary antigen test Human n Introduc oducti tion on Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor 5 Pract ctice ce
Me Mechanism chanism of f Quorum orum Sensing ensing (Competence Stimulating Peptide) DNA uptake and recombination S. pneumoniae Human n Introduc oducti tion on 6 Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor Pract ctice ce
Pneu eumosens mosensor or Over vervie view Human n Pneumosen mosenso sor 7 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
Human n Pneumosen mosenso sor 8 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
Pneu eumosens mosensor or Over vervie view Human n Pneumosen mosenso sor 9 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
De Detection ection Mo Module dule Human n Pneumosen mosenso sor 10 10 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
iae σ x Promot S. S. pn pneumon eumoniae omoter ers s Module dule E. coli protease Human n Pneumosen mosenso sor 11 11 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
Functionality of σ x Promoter σ x Bright Field GFP Scale bar: 5mm | Gain: 4 | Exposure time: 200 ms | Gamma BF: 1 | Gamma GFP: 1 Human n Pneumosen mosenso sor Riboregul ulator tor Conclusi usion Introducti uction 12 Pract ctice ce
Relative Promoter Unit Measurement of P celA and P comFA Relative Promoter Unit σ x Promoter J23101 K1379000 J23101 K1379001 ( P celA ) ( P comFA ) Human n Pneumosen mosenso sor 13 13 Riboregul ulator tor Conclusi usion Introducti uction Pract ctice ce
Human n Ribor oregu egulat lator or 14 14 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Ribosome CDS cis -repressing trans -activating Human n Ribor oregu egulat lator or 15 15 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Ribosome TA CR RBS CDS Human n Ribor oregu egulat lator or 16 16 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Human n Ribor oregu egulat lator or 17 17 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Name of Riboregulator Team Functionality Key1 and Lock1 Berkeley 2005 Yes Key3 and Lock3 Berkeley 2005 Yes Human n Ribor oregu egulat lator or 18 18 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Lock ck 3 and and Key ey 3 Lock ck 1 an and d Key ey 1 Missing TA CR CR&TA Missing TA CR CR&TA Human n Ribor oregu egulat lator or 19 19 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Human n Ribor oregu egulat lator or 20 20 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
DH5 α BW25113 DH10B Human n Ribor oregu egulat lator or 21 21 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
omoter Unit e Promoter ive ativ Relat Human n Ribor oregu egulat lator or 22 22 Conclusi clusion on Introducti uction Pneumo mose senso nsor Pract ctice ce
Human Practice Human an 23 23 Conclusi usion Introducti ction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human an 24 24 Conclusi usion Introducti ction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human an 25 25 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human Practice Projects 2008-2013 3000 + Record Entries Human an 26 26 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human an 27 27 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human an 28 28 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Human an 29 29 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Report Analyze and interpret the data Catch any potential trends Human an Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor 30 Pract actice ice
Types of Projects Done in the Past Human an Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor 31 Pract actice ice
Human an 32 32 Conclusi usion Introducti uction Pneumo mose senso nsor Riboregul ulator tor Pract actice ice
Conclusion Human n Conclu lusi sion on 33 33 Introducti uction Pneumo mose senso nsor Rioregul ulator tor Pract ctice ce
Pneumosensor Characterized P celA and P comFA Showed parts from Gram positive bacteria are functional in Gram negative bacteria. Human n Conclu lusi sion on Introducti uction Pneumo mose senso nsor Riboreg egulato lator 34 Pract ctice ce
Riboregulator Provided additional characterization data for 4 pairs of lock and key CDS Characterized P BAD in 3 different cell strains. Human n Conclu lusi sion on Introducti uction Pneumo mose senso nsor Riboreg egulato lator 35 Pract ctice ce
Ackno cknowledgement wledgement Fundi ding ng Human Practic ice Office of the Provost, HKUST SUSTC-Shenzhen Office of the Dean of Science, HKUST Tec-Monterrey Manchester Discu cussio ssion n and Technical nical Assistance nce Cornell Prof. Siu Chun HUNG Prof. Hon Ming LAM (CUHK) Prof. King L. CHOW and his lab members Prof. Tom RICHARD (PSU) Dr. Samuel YU (HKUST) Reagen ents Ms. Christine CHIU (HKUST) Prof. Patrick Chiu Yat WOO (HKU) Mr. Kevin WONGSO (HKUST) Dr. Don MORRISON Independent Schools Foundation Academy ( Université de Toulouse, UPS, Laboratorie de Microbiologie et Génétique Moléculaires ) Wellcome Trust Sanger Institute Human n Conclu lusi sion on 36 36 Introducti uction Pneumo mose senso nsor Riboreg egulato lator Pract ctice ce
Finding Pneumo HKUST iGEM 2014 TEAM
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