EnVigour HX TM Information Session Michelle Miller, MSc, MBA Chief - - PowerPoint PPT Presentation

Michelle Miller, MSc, MBA

Chief Executive Officer

November 29, 2017

1

EnVigour HXTM Information Session

Delta Genomics

http://canada150in150.ca/browse- stories/community/agriculture/rural-livestock- amazing-graze/93

Delta Genomics

• Incorporated in 2012 as a

Not-for-Profit Organization

• Spun-out of the University
• f Alberta on April 1, 2014
• 9 Current Employees
• Located in downtown

Edmonton (the old Bay Building)

2014: 42,564 samples 2015: 36,315 samples 2016: 48,244 samples 2017: 36,665 samples (end of Q3)

Delta Genomics

Genomics will influence every industry

The Goal of Delta Genomics

To increase the profitability, competitiveness, and sustainability of the Canadian Livestock Industry

• In the Dictionary: the branch of molecular biology

concerned with structure, function, evolution, and mapping of genomes

• In Practice: a holistic approach to genetic testing

(can answer more complex questions)

What is Genomics?

Genetic Testing: Red or Black?

Genetic Testing vs Genomics

Genetic Testing: Horned or Polled?

Genetic Testing vs Genomics

Genetic Testing: Parentage or Not?

Genetic Testing vs Genomics

Genomics: How do I select the best replacements?

Genetic Testing vs Genomics

EnVigour HXTM

A Made in Canada Genomic Tool for Crossbred Cattle

EnVigour HXTM: Genomics as a Tool in Replacement Selection

EnVigour HXTM – What it Does

• 1. Parentage
• 2. Genomic Breed Composition Analysis
• 3. Vigour Score: measurement of heterosis/hybrid

vigour Three services in one package for $45

EnVigour HXTM – Results Example

EnVigour HXTM Parentage Applications

• Determine bulls that are not producing
• Identify bulls that produce good calves
• Prevent inbreeding
• Herd bull annual maintenance: $18001

1Western Beef Development Centre

EnVigour HXTM Breed Composition Applications

• Optimize breed complementarity
• You picked the breeds in your herd because you like the

positive attributes of those breeds

• Cow-Bull Matching
• Replacement heifer: $12501
• Branded beef programs(?)

1Western Beef Development Centre

EnVigour HXTM Breed Composition Applications

10 Breeds can be identified with EnVigour HXTM:

Angus Charolais Galloway Gelbvieh Hereford Limousin Maine-Anjou Salers Shorthorn Simmental

Genomic Breed Composition

• n

• m

• s

• n

• m

• b

• m

• s

• n

• s

• e

• 4

• s

The red circles are calves that the cows switched; record keeping is an excellent tool but will never be perfect (even in a research herd)

Inheritance of DNA & Recombination

Sire – Angus 100% Dam – Hereford 100% F1 Progeny Expected – AN50%; HE50% F1 Progeny Actual – AN50%; HE50%

Adapted from Mehdi Sargolzaei and Steve Miller, University of Guelph

Bovine Genome 30 pairs of chromosomes ~3,000,000,000 bp ~34,000,000 variants ~ 30,000 genes

Recombination is a phenomenon that occurs between every generation (even in people) and is essential to maintaining diversity within a species. It involves mixing of DNA that an individual received from their mom and dad before passing that DNA to the next generation.

Sire – Simmental 100% Dam – AN50%; HE50% Progeny Expected – SM50%; AN25%; HE25% Progeny Actual – SM50%; AN15%; HE35%

Adapted from Mehdi Sargolzaei and Steve Miller, University of Guelph

Inheritance of DNA & Recombination

In this example, the calf that resulted from this crossing only has 15% Angus DNA instead of the predicted 25% Angus DNA. If these types of recombination events occurred for a couple more generations, it’s easy to see how the Angus DNA could be bred out of this family.

Record keeping will put you in the right ballpark, but recombination may offer some surprises

EnVigour HXTM Breed Composition Applications

Guess this heifer’s breed composition

EnVigour HXTM Breed Composition Applications

D6574

Angus: 53% Simmental: 40% Charolais: 6% Indeterminate: 1% Vigour Score 56%

EnVigour HXTM Breed Composition Applications

Guess this heifer’s breed composition

EnVigour HXTM Breed Composition Applications

D6543

Angus: 80% Simmental: 14% Charolais: 4% Indeterminate: 2% Vigour Score 33%

EnVigour HXTM Hybrid Vigour Applications

• Not a secret in the beef industry: Crossbreeding is the norm

across North America

• Enables selection of heifers with high Vigour Scores
• Improve fitness traits (per 10% increase in Vigour Score)1:
• 200-day Weaning Weight: 3lbs increase
• Age at First Calving: 2 days earlier
• Pregnancy Rate: 2% increase
• Weaning Rate: 3% increase

1Basarab et al. 2017. in preparation

Increasing Vigour Scores

Vigour Score Breed X Breed Y Bull X Bull Y Year 1 38% 25% 75% Year 2 47% 63% 37% 100% 0% Year 3 43% 31% 69% 0% 100% Year 4 45% 66% 34% 100% 0% Year 5 44% 33% 67% 0% 100% Vigour Score Breed X Breed Y Bull X Bull Y Year 1 38% 25% 75% Year 2 47% 63% 37% 100% 0% Year 3 30% 81% 19% 100% 0% Year 4 17% 91% 9% 100% 0% Year 5 9% 95% 5% 100% 0% EnVigour HXTM Guided Selection: Two-Way Cross Consistently use Bull X

Increasing Vigour Scores

Vigour Score Breed X Breed Y Bull X Bull Y Year 1 38% 25% 75% Year 2 47% 63% 37% 100% 0% Year 3 43% 31% 69% 0% 100% Year 4 45% 66% 34% 100% 0% Year 5 44% 33% 67% 0% 100% Vigour Score Breed X Breed Y Bull X Bull Y Year 1 38% 25% 75% Year 2 22% 13% 87% 0% 100% Year 3 12% 6% 94% 0% 100% Year 4 6% 3% 97% 0% 100% Year 5 3% 2% 98% 0% 100% EnVigour HXTM Guided Selection: Two-Way Cross Consistently use Bull Y

Increasing Vigour Scores

Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 57% 10% 35% 55% 0% 0% 100% Year 3 59% 55% 18% 27% 100% 0% 0% Year 4 56% 28% 59% 13% 0% 100% 0% Year 5 57% 14% 29% 57% 0% 0% 100% EnVigour HXTM Guided Selection: Three-Way Cross Consistently use Bull X Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 52% 60% 35% 5% 100% 0% 0% Year 3 33% 80% 18% 2% 100% 0% 0% Year 4 18% 90% 9% 1% 100% 0% 0% Year 5 10% 95% 4% 1% 100% 0% 0%

Increasing Vigour Scores

Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 57% 10% 35% 55% 0% 0% 100% Year 3 59% 55% 18% 27% 100% 0% 0% Year 4 56% 28% 59% 13% 0% 100% 0% Year 5 57% 14% 29% 57% 0% 0% 100% EnVigour HXTM Guided Selection: Three-Way Cross Consistently use Bull Y Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 27% 10% 85% 5% 0% 100% 0% Year 3 14% 5% 93% 2% 0% 100% 0% Year 4 7% 3% 96% 1% 0% 100% 0% Year 5 4% 1% 98% 1% 0% 100% 0%

Increasing Vigour Scores

Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 57% 10% 35% 55% 0% 0% 100% Year 3 59% 55% 18% 27% 100% 0% 0% Year 4 56% 28% 59% 13% 0% 100% 0% Year 5 57% 14% 29% 57% 0% 0% 100% EnVigour HXTM Guided Selection: Three-Way Cross Consistently use Bull Z Vigour Score Breed X Breed Y Breed Z Bull X Bull Y Bull Z Year 1 46% 20% 70% 10% Year 2 57% 10% 35% 55% 0% 0% 100% Year 3 37% 5% 18% 77% 0% 0% 100% Year 4 20% 3% 9% 88% 0% 0% 100% Year 5 11% 1% 4% 95% 0% 0% 100%

EnVigour HXTM Increasing Vigour Scores Summary

• Match your bulls with cows that are least like them
• ie: Put the mostly Angus cows with the Simmental or Hereford bulls
• Breed Composition and Vigour Score are permanent
• Can increase Vigour Score with strategic crossbreeding

Caveat

• Know your goals!
• Which breed composition do you want in your cows?
• Does your perfect breed composition align with the final product that

your customers want to pay for?

EnVigour HXTM Case Study: Lacombe Research Farm

412 Replacement Heifers were followed over 5 calvings (1050 matings) then split into High Vigour and Low Vigour groups

Basarab et al. 2017. in preparation

Basarab et al. 2017. in preparation

EnVigour HXTM An Investment

EnVigour HXTM A Cautious Investment

• The easiest part is sending

in samples and getting results

• Implementation of results is

required to see a return on your investment – that means sorting cows with bulls; potentially a lot of cows

EnVigour HXTM Return on a Very Cautious Investment

Test 30 heifers from 100 cows in your herd annually for 5 years Two-Way Crosses: EnVigour HXTM Selection vs Bull X Only

Vigour Score Δ Weaned Calves Δ Income Δ Repl Female Δ Repl Cost Δ Net Income Δ Annual EnVigour HX Cost Net Gain Year 1 0% $0 $0 $0 $1350

• $1350

Year 2 0% $0 $0 $0 $1350

• $1350

Year 3 4% 1 $974 $0 $974 $1350

• $376

Year 4 8% 2 $1947

• 1
• $1250

$3197 $1350 $1847 Year 5 11% 3 $2921

• 2
• $2500

$5421 $1350 $4071 TOTAL 6 $5842

• 3
• $3750

$9592 $6750 $2842

Breakeven: Year 5 Average Gain: $6 per cow per year

EnVigour HXTM Return on a Very Cautious Investment

Test 30 heifers from 100 cows in your herd annually for 5 years Three-Way Crosses: EnVigour HXTM Selection vs Bull X Only

Vigour Score Δ Weaned Calves Δ Income Δ Repl Female Δ Repl Cost Δ Net Income Δ Annual EnVigour HX Cost Net Gain Year 1 0% $0 $0 $0 $1350

• $1350

Year 2 2% $0 $0 $0 $1350

• $1350

Year 3 8% 2 $1947

• 1
• $1250

$3197 $1350 $1847 Year 4 11% 3 $2921

• 2
• $2500

$5421 $1350 $4071 Year 5 14% 4 $3894

• 2
• $2500

$6394 $1350 $5044 TOTAL 9 $8762

• 5
• $6250 $15,012

$6750 $8262

Breakeven: Year 4 Average Gain: $17 per cow per year

EnVigour HXTM Return on a Cautious Investment

Test 100 cows in Year 1, then 30 Replacement Heifers Annually Two-Way Crosses: EnVigour HXTM Selection vs Bull X Only

Vigour Score Δ Weaned Calves Δ Income Δ Repl Female Δ Repl Cost Δ Net Income Δ Annual EnVigour HX Cost Net Gain Year 1 0% $0 $0 $0 $4500

• $4500

Year 2 0% $0 $0 $0 $1350

• $1350

Year 3 13% 3 $2921

• 2
• $2500

$5421 $1350 $4071 Year 4 28% 8 $7788

• 5
• $6250

$14,038 $1350 $12,688 Year 5 35% 10 $9735

• 7
• $8750

$18,485 $1350 $17,135 TOTAL 21 $20,444

• 14
• $17,500 $37,944

$9900 $28,044

Breakeven: Year 4 Average Gain: $56 per cow per year

EnVigour HXTM Return on a Cautious Investment

Test 100 cows in Year 1, then 30 Replacement Heifers Annually Three-Way Crosses: EnVigour HXTM Selection vs Bull X Only

Vigour Score Δ Weaned Calves Δ Income Δ Repl Female Δ Repl Cost Δ Net Income Δ Annual EnVigour HX Cost Net Gain Year 1 0% $0 $0 $0 $4500

• $4500

Year 2 5% 1 $974 $0 $974 $1350

• $376

Year 3 26% 7 $6815

• 5
• $6250

$13,065 $1350 $11,715 Year 4 38% 11 $10,709

• 7
• $8750

$19,459 $1350 $18,109 Year 5 48% 14 $13,629

• 9
• $11,250 $24,879

$1350 $23,529 TOTAL 33 $32,127

• 21
• $26,250 $58,377

$9900 $48,477

Breakeven: Year 3 Average Gain: $97 per cow per year

EnVigour HXTM A Cautious Investment

• Start with your bulls and

replacement heifers (after removing bad feet, bad udders, bad legs, and other red flags)

• Or start with Parentage
• Develop management

practices for sorting heifers and cows with appropriate bulls on a small scale first

• If you can’t sort breeding

groups, EnVigour HXTM will identify the calves that resulted from your ideal matings

EnVigour HXTM Getting Started

1 2 3

www.deltagenomics.com

EnVigour HXTM Getting Started with Support

• Students at the Olds College

Technology Access Centre are supporting EnVigour HXTM services and implementation – for FREE

• Students will also come pull

samples for a day (Fee Applies)

• Contact: Kaley Segboer

ksegboer@oldscollege.ca 403-556-4786

EnVigour HXTM Getting Started with Herdtrax

• EnVigour HXTM orders can

be placed through Herdtrax

• Results will be returned

automatically to your Herdtrax account

• Contact: Troy Drake

info@herdtrax.com 1-844-321-HERD (4374)

EnVigour HXTM Sample Types

The Good

How to Take a Hair Sample

• From the Tail Switch
• Wrap some hair around

your finger

• Pull up towards to top
• f the tail
• 30-40 follicles

Plenty of really good YouTube videos

Getting Samples to the Lab

• Regular mail or courier

if you’re in a hurry

• Blood vials and raw

tissue needs to be kept cold and shipped fast

• Semen straws need to

be kept intact

The Laboratory Process

Sample Inventory Sample Inventory DNA Extraction DNA Extraction Testing Testing Data Analysis Data Analysis

10 days to 25 days

EnVigour HXTM Results

EnVigour HXTM Results

Delta Genomics Herdtrax Olds College TAC

EnVigour HXTM in Summary

• EnVigour HXTM is a tool for selecting replacement

females that:

• 1. Have good Vigour Scores indicating that they have the

genetic potential to get pregnant and stay in the herd

• 2. Have a breed composition that compliments your bulls so

you can maintain the balance of breeds you use and ultimately produce the final product that your customer wants to pay for

In Summary: Genomics is a Tool

Thank-you!

Research Team

• Dr. John Basarab
• Dr. Mohammad Abo-Ismail
• Dr. John Crowley
• Dr. Graham Plastow
• Dr. Donagh Berry

Dawn Trautman