Does DNA methylation facilitate phenotypic plasticity in marine - PowerPoint PPT Presentation

Does DNA methylation facilitate phenotypic plasticity in marine invertebrates? Steven Roberts School of Aquatic and Fishery Sciences robertslab.info @sr320

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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?

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Lab Background commercially important traits

Lab Background commercially important traits modified from

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Epigenetics Histone Modification short RNAs DNA Methylation

Epigenetics Photo credit: Flickr, Creative Commons, he-boden

Epigenetics

m m DNA Methylation

Function?

Non- Vertebrates? landscape and function is very different than what is observed in vertebrates

Non- Vertebrates? landscape and function is very different than what is observed in vertebrates

Non- Vertebrates? landscape and function is very different than what is observed in vertebrates

Absent in several model organisms

Function? Oysters?

mosaic associated with gene bodies

in silico analysis Roberts and Gavery 2012

in silico analysis Roberts and Gavery 2012

in silico analysis Roberts and Gavery 2012

Whole Genome BS-Seq Two Lineages - Sperm + Larvae (72h & 120h) Claire Olson

Differentially Methylated Loci predominant in Transposable Elements Claire Olson

Claire Olson github.com/sr320/olson-dev

Summary • Sparsely (~16 %) methylated genome • Gene body methylation • Function specific • TEs are not hypermethylated across genome • Preliminary evidence indicates DMRs are predominant in TEs

Role?

In species that experience a diverse range of environmental conditions, processes have evolved to increase the number of potential phenotypes in a population in order to improve the chances for an individual’s survival.

in silico analysis Roberts and Gavery 2012

Stochastic Variation response to housekeeping change

Mackenzie Gavery

response to change housekeeping

Targeted Regulation

Mackenzie Gavery

Mackenzie Gavery

Transposable Elements providing increased diversity?

Beginning to test these Hypotheses modified from

Very new data Environment and gene expression stochastic or targeted?

Very new data Environmental impact (Estrogens)

Very new data Ocean Acidification Selection Environmental Impact Katie Lotterhos

Very new data Heritability Plasticity Common Garden Experiment Local Adaptation Genetics versus Epigenetics

Acknowledgements Mackenzie Gavery EPA Claire Ellis DNA methylation STAR Sam White Bill Howe Dan Halperin robertslab.info slides, data & more @