Degradation Degradation Aggregation Goals For The Summer Address - PowerPoint PPT Presentation

Degradation

Degradation Aggregation

Goals For The Summer

Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan

Create Novel Device to Mimic Oceanic Force

Pioneer Marine Chassis for the Registry

Question Access to the Registry with First Public Biobrick

Conan the Bacterium: The Ancient Microscopic Hero.” Chaotic Utopia. 2006. 16 Feb 2007.

Growth Rates In Different Salt Concentrations Growth during exponential phase [h ] -1 LB + 0.3M NaCl LB + 0.6M NaCl LB

Our BioBrick Grows Better Comparing Increase in Exponential Growth Rate Between Biobricks of UCL ‘12 and TU Delft ‘10 in Salt Supplemented Media Percentage Increase in Growth Rates Salt Concentration (M) Our biobrick showed improved growth rates for higher salt concentration than the TU Delft ‘10 biobrick

from The Basics of Biology by Great Pacific Media

Obtaining Nuclease Synthesise the Nuclease (from Streptococcus Aureus) and dsbA Sequence (Which Transports Nuclease to the Periplasm)

Sequence Design Results in the Transport of Nuclease to Periplasm

BioBrick Toxicity Growth Curve Assay OD (600nm) Time (Hours) BBa_K729019 W3110 (Control)

DNAse Agar Assay

Correlation Between Colony and Halo Diameter for Nuclease Expession of BBa_K729019 Halo Diameter (mm) Colony Diameter (mm)

Halo Diameter (m) Against Mass of Colony (kg) Halo Diameter (m) Mass of Colony (kg)

Use of NahR for Indirect Detection

R O T A N I M R E T R H A N B0015 S B R E V I T U T K228004 I T S N O C R E T O M O B0034 R P J23119 P O OP OP O P R h a N Plastic Betts, K., “Why small plastic particles may pose a big problem in the oceans”, Env. Sci. & Tech. 42 (24), 2008

Triggering Mechanism R h a N P O

Curli Construct Design

Congo Red Agar Assay

Shear Device Principle Shear = N - rotational speed Shear Force r - radius - distance between the discs x - viscosity of fluid μ Radius from Centre

Denny, M., “Wave forces on intertidal organisms: A case study’”, American Society of Limnology and Oceanography, Inc., 30(6), 1985, 1171-l 187

Melbourne iGEM 2007

Staying In The Zone Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing on the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601

Improved Biobrick Shows a Ten-Fold Increase in Fluorescence Fluorescence Fluorescence Time (min) Time (min) GFP T7 RNAP GFP

Our Improved Biobrick Shows a Three-Fold Better Expression of Fluorescence Over Previous Constructs Relative Fluorescence Time

Measurement of Extracellular Lacasse Activity Using a Syringaldazine Assay OD (530nm) Time (min) Control Laccase Positive Activity of Laccase-Positive Cells is 20-Fold Higher Than Control Cells

High Magnification Low Magnification (10 000x) (100x) Water SEM Results W3110 Lacasse

Measurement of Extracelluar Lacasse Activity Using a Syringaldazine Assay Collaboration with Bielefeld (BBa_K863005) OD (530nm) BL21 + Lacasse W3110 + Lacasse W3110 (Untransformed) Time (mins)

Human Practice

Ethics and Legal UN Legal Feedback Online Discussions Feedback from British Science Association Crowdfunding Speed Debate Public BioBrick iGEM Day Open Day Meeting of Young Minds Family Engagement gemFM Modelling Workshop for LMU iGEM Living Architecture UK Meet-Up Characterisation Paris Collaboration Collaboration with Bielefeld Outreach CAS Conference iGEM community

radio video

Ethics and Legal UN Legal Feedback Online Discussions Feedback from British Science Association Crowdfunding Speed Debate Public BioBrick iGEM Day Open Day Meeting of Young Minds Family Engagement gemFM Modelling Workshop for LMU iGEM Living Architecture UK Meet-Up Characterisation Paris Collaboration Collaboration with Bielefeld Outreach CAS Conference iGEM community

New Measurement Device

14 New BioBricks

14 New BioBricks + 1

Degradation Degradation Aggregation Goals For The Summer Address - PowerPoint PPT Presentation

Degradation Degradation Aggregation Goals For The Summer Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan

Zhang Logo Background n-alkane degradation ? n-alkane degradation Degradation Sensing

PV module degradation: the impact of light induced degradation (LID) and how to fix it! Dr.

The Economics of Land Degradation (ELD) Initiative Economic arguments to combat land degradation

Graceful Degradation Fault-tolerance, or graceful degradation, is the property that enables a

Aggregation and Degradation in JetStream: Streaming analytics in the wide area Ariel Rabkin,

Aggregation and Degradation in JetStream: Streaming Analytics in the Wide Area Ariel Rabkin

Can we get more fish? Degradation and recovery of fisheries resources Degradation and recovery of

The Audio Degradation Toolbox http://code.soundsoftware.ac.uk/projects/audio-degradation-toolbox/

Land Degradation Land Degradation Assessment in Cambodia Sovuthy Pheav, PhD (Agric. Sci.) Di

DEGRADATION in E.coli iGEM 2012_ UC Davis 1 Introduction PET Degradation Protein Engineering

Neutrality and transformation? Land Degradation Neutrality & possible chances for land

Temperature accelerated Degradation of GaN HEMTs under High power Stress: Activation Energy

Evaluation of Degradation Studies of Evaluation of Degradation Studies of Veterinary Drugs in

Drying-up of Rivers Wetland degradation 40% of the total rivers was the rate reaches 90%

Habitat loss and degradation PVA: Essay 12.2 (and adjacent text) Loss: Irreversible damage of a

DNA RNA Protein synthesis AMINO ACIDS PROTEIN Protein degradation FUNCTION Some properties

Potential Solar Sail Degradation Effects on Trajectory and Attitude Control Bernd Dachwald 1 and

Land Degradation and Restoration. Outline 1. Introduction to IPBES (item 1) Organisation of

SOLAR CELL/MODULE DEGRADATION AND FAILURE DIAGNOSTICS T.J. McMahon National Renewable Energy

Dilution, degradation, and time delays in algebraic models Matthew Macauley Department of

Dilution, degradation, and time delays in Boolean network models Matthew Macauley Department of

Actionable Science on Fate and Transport and Degradation and Remediation of Per- and

and -XRD for the characterization and degradation of chrome yellow pigments: a focus on

Kaposis Sarcoma-Associated Herpesvirus Transactivartor RTA Promotes Degradation of the

Degradation Degradation Aggregation Goals For The Summer Address - PowerPoint PPT Presentation

Degradation Degradation Aggregation Goals For The Summer Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan

Zhang Logo Background n-alkane degradation ? n-alkane degradation Degradation Sensing

PV module degradation: the impact of light induced degradation (LID) and how to fix it! Dr.

The Economics of Land Degradation (ELD) Initiative Economic arguments to combat land degradation

Graceful Degradation Fault-tolerance, or graceful degradation, is the property that enables a

Aggregation and Degradation in JetStream: Streaming analytics in the wide area Ariel Rabkin,

Aggregation and Degradation in JetStream: Streaming Analytics in the Wide Area Ariel Rabkin

Can we get more fish? Degradation and recovery of fisheries resources Degradation and recovery of

The Audio Degradation Toolbox http://code.soundsoftware.ac.uk/projects/audio-degradation-toolbox/

Land Degradation Land Degradation Assessment in Cambodia Sovuthy Pheav, PhD (Agric. Sci.) Di

DEGRADATION in E.coli iGEM 2012_ UC Davis 1 Introduction PET Degradation Protein Engineering

Neutrality and transformation? Land Degradation Neutrality &amp; possible chances for land

Temperature accelerated Degradation of GaN HEMTs under High power Stress: Activation Energy

Evaluation of Degradation Studies of Evaluation of Degradation Studies of Veterinary Drugs in

Drying-up of Rivers Wetland degradation 40% of the total rivers was the rate reaches 90%

Habitat loss and degradation PVA: Essay 12.2 (and adjacent text) Loss: Irreversible damage of a

DNA RNA Protein synthesis AMINO ACIDS PROTEIN Protein degradation FUNCTION Some properties

Potential Solar Sail Degradation Effects on Trajectory and Attitude Control Bernd Dachwald 1 and

Land Degradation and Restoration. Outline 1. Introduction to IPBES (item 1) Organisation of

SOLAR CELL/MODULE DEGRADATION AND FAILURE DIAGNOSTICS T.J. McMahon National Renewable Energy

Dilution, degradation, and time delays in algebraic models Matthew Macauley Department of

Dilution, degradation, and time delays in Boolean network models Matthew Macauley Department of

Actionable Science on Fate and Transport and Degradation and Remediation of Per- and

and -XRD for the characterization and degradation of chrome yellow pigments: a focus on

Kaposis Sarcoma-Associated Herpesvirus Transactivartor RTA Promotes Degradation of the

Neutrality and transformation? Land Degradation Neutrality & possible chances for land