DEVELOPING A COMPACT AND LOW- COST LONG-TERM LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE (SCBE06) CHUA YA XUAN MENTOR: DR PUI TZE SIAN
INTRODUCTION • LIVING THINGS ARE MADE UP OF CELLS AND CELLS ARE MADE UP OF THOUSANDS OF MOLECULES THAT INTERACT WITH EACH OTHER. • IN ORDER TO MAKE NEW DISCOVERIES ABOUT THE CELLULAR DYNAMICS AND DEVELOP DRUGS TO COMBAT DISEASES THAT ARISE FROM DYSFUNCTIONAL CELLS, SCIENTISTS ARE REQUIRED TO STUDY THE CONSTANTLY CHANGING PATTERNS OF THESE MOLECULES
CURRENT METHODS • LONGITUDINAL STUDIES • TIME LAPSE MICROSCOPY
LIMITATIONS CELLS ARE NOT IN BULKY COSTLY OPTIMUM CONDITION
AIM • MAKE USE OF 3D PRINTING, THE PROTOTYPING TECHNOLOGY, TO COME UP WITH A COMPACT , LOW-COST AND EASY-TO-OPERATE LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE
ENGINEERING GOALS FUCTIONAL PHYSICAL COMPACT LOW-COST TEMPERATURE HUMIDITY
METHODOLOGY - HOUSING - Temperature & Holder for humidity sensor CO 2 sensor • CAD software • Acrylic glass 3D MODEL OF THE TOP VIEW OF INCUBATOR 3D MODEL OF THE SIDE VIEW OF INCUBATOR
METHODOLOGY - MICROCONTROLLER - • Arduino open-source Integrated Development Environment • C++ coding language
METHODOLOGY - INCUBATOR SYSTEM - Close-up view of incubator Top-view of the entire physical set-up Close-up view of LED panel
RESULTS - TEMPERATURE - • From a temperature of 24.4 ˚C, it took 85 minutes for the temperature to rise to 28 ˚C, the optimum temperature we aim to sustain throughout the experiment. Graph of temperature against time to test how long the incubator takes to reach the ideal temperature of 28 ˚C
RESULTS - TEMPERATURE - • Temperature only increases by 0.5˚C in the next hour from 28˚C. Graph of temperature against time to test the stability of the incubator in maintaining the optimum temperature
RESULTS - TEMPERATURE - STEINHART-HART EQUATION • Software-based feedback loop using 𝟐 𝑼 = 𝑸 + 𝑪 + 𝑸 𝟒 × 𝑫 + 𝑩 − 𝟑𝟖𝟒. 𝟐𝟔 the mean temperature • Used to sustain temperature at 28 ˚C T: Temperature in degree Celsius A,B,C are Steinhart-Hart coefficients A: 1.129148 × 10 -3 B: 2.34125 × 10-4 C: 8.76741 × 10 -8 𝑄 = ln 𝑆𝑞𝑏𝑗𝑠𝑓𝑒 × 𝑊𝑡𝑣𝑞𝑞𝑚𝑧 − 𝑆𝑞𝑏𝑗𝑠𝑓𝑒 𝑊𝑝𝑣𝑢
RESULTS - HUMIDITY - • 2 wells with 10ml of water each were used during the experiment • Humidity level of 85%-95% is ideal • At the optimum temperature of 28 ˚C, humidity increases from 86.8% to 90.3%. Graph of humidity against time to test the ability of the incubator in maintaining an optimal humidity range
DESIGN SPECIFICATIONS Incubator Incubators on designed the market (average) Size 17.5 cm x 28 cm x 12 cm x 7 cm 20 cm x 9 cm 0.654g 1.5 kg Cost <$120 $15000-$20000 with microscope
CONCLUSION ENGINEERING GOALS PHYSICAL FUCTIONAL COMPACT LOW-COST TEMPERATURE HUMIDITY
FUTURE IMPROVEMENTS HEATING ELEMENT VENTILATOR
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