Compound Light Microscope Light & glass lenses used to - PowerPoint PPT Presentation

Compound Light Microscope Light & glass lenses used to magnify objects

Eyepiece 10X Objective lenses: 4x,10x, 40x Up to 2000x

Resolution Ability of microscope to show fine detail

Wet Mounts  Cover slips: protect lens from water, keep specimen in place & protect them  Field of View: how much of the object you can see  stains

ScanningElectronMicroscope SEM M______ lenses and electrons used to magnify objects

pollen 3D 100- 300,000X $30-100,000 Metal coating

Transmission electron microscope

Wasp virus 2D Up to 2,000,000X $100- 200,000 Metal coatings

Microtome (Uses diamond knife to cut very thin slices of specimen)

Centrifuge to spin at high speeds; breaks up cells; separates by density

Cell Fractionation

Cell Culture (to clone identical cells) HeLa cell line http://www.youtube.com/watch?v=2mOroGqJ_Uk

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