Carlos Calvo Garrido Agreenskills Meeting, 12-14 October 2015
SUMMARY Background The subject: Botrytis bunch rot and biological control The project: Bacterial biological control agents against Botrytis bunch rot and their effects on grape berry microbiota Expected Outputs .02 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
BACKGROUND 2006 - Major Degree in Biology by the Autonomous University of Madrid (Spain) Ecology, Evolutive Biology and Biodiversity Research work on organic agriculture. 2010 - Master in Research on Agricultural and Food Production by the University of Lleida (Spain) Research work on biological control of fruit pathogens using antagonist microorganisms. Universitat de Lleida 2013 - PhD in Food and Agriculture Science and Technology by the University of Lleida (Spain) Postharvest Pathology group at IRTA-Lleida (Catalan Institute for Research & Technology in Food & Agriculture; Spain) Thesis title: Efficacy of alternative strategies to synthetic fungicides to control of Botrytis bunch rot ( Botrytis cinerea ) in organic wine grapes Thesis supervisors: Dr. Neus Teixidó and Pr. Inmaculada Viñas). .03 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
BACKGROUND PhD in Food and Agriculture Science and Technology by the University of Lleida (Spain) Other research topics : • B. cinerea epidemiology • Survival of Biological Control Agents (BCAs) under abiotic factors • Sour rot of grapes. Research stays: • 2011 - The New Zealand Institute for Plant and Food Research Ruakura Research Centre, Hamilton, New Zealand - 5 months • 2012 – Institut National de la Recherche Agronomique (INRA) Centre Bordeaux-Aquitaine, Villenave d’Ornon , France – 6 months .04 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
BACKGROUND Calvo-Garrido et al. (2014), Pest Management Science 70 : 922-930 Calvo-Garrido et al. (2014), Biological Control. 79 : 24-35 Calvo-Garrido et al. (2014), Journal of Applied Microbiology. 117: 800-811 Calvo-Garrido et al. (2014), Journal of Applied Microbiology 116 : 967-979 Calvo-Garrido et al. (2014), Pest Management Science 70 : 595-602 Calvo-Garrido et al. (2013), Plant Pathology 62 : 510-519. Calvo-Garrido et al. (2013), Letters in Applied Microbiology. 57 : 356-361 .05 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
_01 Botrytis bunch rot and biological control .06 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Botrytis cinerea Filamentous fungi Necrotrophic pathogen Diverse substrates Many economically important plant hosts .07 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Botrytis cinerea Grapevine: Botrytis bunch rot (BBR) Quantity losses Quality losses (Ky et al. 2013) .08 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
BBR Control Synthetic fungicide application Development of pathogen resistance Negative effects on environment and health Fungicide use reduction and Alternative control strategies Cultural practices Decision Support Systems / Disease Risk Models Natural products Biological control Combinations! .09 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
_03 Bacterial biological control agents against Botrytis bunch rot and their effects on grape berry microbiota .010 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Bacterial BCAs and Microbiota Bacterial Biological Control Agents Project title: Biocontrol strategies against Botrytis in vineyards based on management indicators and developing Biological Control Agents Time period: 3 years Funding: Bordeaux Wine Council (CIVB) Partnerships: IFVV + Chambre Agriculture Gironde + INRA INRA Bordeaux-Aquitaine Research Unit: SAVE (Vineyard Agroecology and Plant Health) INRA Department of Health-Plant-Environment (SPE) .011 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Bacterial BCAs and Microbiota Bacterial Biological Control Agents Project title: Biocontrol strategies against Botrytis in vineyards based on management indicators and developing Biological Control Agents Time period: 3 years Funding: Bordeaux Wine Council (CIVB) Partnerships: IFVV + Chambre Agriculture Gironde + INRA Objectives: 1) to test field efficacy of bacterial BCAs, developing recommendations for vineyard applications based on a BBR risk model, 2) to develop specific BCA formulations, using natural products to achieve higher efficacy and best BCA survival in the field 3) to examine the effects of BCA applications on berry microbiota. .012 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Bacterial BCAs and Microbiota Bacterial Biological Control Agents Previous research: Screening of bacteria isolated from grapevine wood and berries against B. cinerea and Neofusicocum parvum Haidar et al . (in press). Biological Control. Characterisation mode of action + Isolates selection for Survival studies field applications + Combination with additives .013 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Bacterial BCAs and Microbiota Bacterial Biological Control Agents Field efficacy: Application on key phenological stages Application based on Disease Risk Model Post-véraison infection model (Ciliberti et al ., in press; Phytopathology) 8 B. cinerea isolates 2 cultivars T x RH combinations Combination with Natural Products Larger scale applications on commercial vineyards (2017) .014 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Bacterial BCAs and Microbiota Bacterial Biological Control Agents Determination of the effects of bacteria massive applications on grape berry natural microbiota Comparison of microbial communities in treated or untreated plots by molecular biology methods: Molecular Footprint (SSCP) Metagenomics (Next-generation Sequencing) Study effect on: fermentative yeasts (wine tipicity), potential BCAs, pathogenic microorganisms… .015 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Other projects Developing alternative strategies against BBR for growers Project title: BIOBOT, Optimisation of Botrytis bunch rot biocontrol, integrated and global disease management Time period: 3 years Funding: ONEMA – Ministry of Agriculture (France) Partnership: IFVV + Chambre Agriculture Gironde + INRA Vineyards: Organic winegrowers network of Aquitaine Objectives: Reference on registered products Determine factor for successful application Optimise its use with the help of other indicators Transfer techniques to growers Bacterial BCAs? .016 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
_04 Expected Outputs .017 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Expected Outputs Development of new bacterial BCAs (At which stage?) Transfer of alternative strategies to growers Scientific publication Bacterial BCAs and Microbiota project results BIOBOT project results Collaboration for publishing other research of the work team (PhD Rana Haidar) New personal research skills: Metagenomics Microbial ecology Vineyard expertise and disease indicators Disease risk modelling .018 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Thanks for your attention! .019 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
Thanks for your attention! .020 Agreenskills Meeting, 12-14 October 2015 Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT
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