by by
play

By By Shaimaa Moustafa Mahdye Eldieghdye lecturer at Biological - PowerPoint PPT Presentation

May 11, 2023 •253 likes •885 views

By By Shaimaa Moustafa Mahdye Eldieghdye lecturer at Biological Application Dep. Atomic Energy Authority 1- Problem*diabetes* 2- Complications of diabetes

  2. By By Shaimaa Moustafa Mahdye Eldieghdye lecturer at Biological Application Dep. Atomic Energy Authority

  3. 1- Problem*diabetes* ضرمءادلاىركسلا 2- Complications of diabetes لكاشملاةجتانلانعةباصلباىركسلاب 3- Treatments of diabetic wound which being used and its side effects تاجلبعلاهمدختسملاايلاحاهراثاوةيبناجلا 4-Our new treatment (plasma) جلبعلاحرتقملامادختسابامزلببلا 5-Wound healing using plasma جئاتنلايتلامتاهقيقحتمادختسابامزلببلا

  5. • ىركسلا ءادلا ضرم • Diabetes is the most common endocrine diseases with a prevalence of more than 8 million people in Egypt. • The most common types ( type 1 & 2) Reabsorption Secretion Over 80% of the filtrate This includes is reabsorbed to blood. active secretion of separated from substances to This ensures that useful blood cells eliminate toxins materials such as and responsible glucose & amino acids for electrolyte are returned to the balance blood

  7. Main causes of diabetes complications

  8. Complications of Diabetes • ىركسلاب ةباصلبا نع ةجتانلا لكاشملا • 1- Nephropathy toxicity • 2-Eye damage • 3-Delaying wound healing • 4- Cardiovascular diseases

  9. • حورجلا مائتلإ Wound healing ➢ Protects the individual from infection & dehydration ➢ Critical to the survival ➢ It has specific phases which include

  10. حورجلا مائتلا لحارم

  12. ✓ Access to care: Wounds Have a Golden *Hour* • so from the onset of the wound … • patients need wound care sooner than • Definitive care at or before 4 weeks with the introduction of advanced therapy to treat the wound Is very important.

  13. DFUs) Complications of Diabetic Foot Ulcers • DFUs that persist more than 4 weeks have 5- fold higher risk of infection. 1 Development of an infection in a foot ulcer increases the risk for hospitalization 55.7 times and the risk for amputation 155 times. ا ل رت و ح • “ Diabetes Neuropathy Foot Ulcer Amputation Infection

  14. Amputations are a serious predictor of death …

  15. Controlling impaired wound healing حورلا ما تلا مدع مكحتلا Prevention & continuing control of impaired wound healing are important, Various methods have been suggested to reduce impaired wound healing, but Usage Side effects

  16. What if ? و ملا 1-No harming tissue or causing pain? ت ل ا 2-Wound healing with low cost ? 3-Disinfect wounds without antibiotics? م ت و ا ا م و ح 4- Enhance healing at short time ? مائتلا ل ا و مم 5- Enhance healing without side effects ? مائتلا و را ا اج So, New methods were needed

  17. plasma The forth state of matter

  18. Plasma What is a Plasma? • 4th State of Matter

  19. The forth state of matter why called plasma It is like the plasma of blood in its majority of components

  20. Plasma components

  21. Main types of plasma according to plasma temp. • Non-thermal plasma (Cold) – used safely at room temperature & atmospheric pressure. • Thermal – plasma are obtained when electron and gas temperature are equal and with very high power and have bad effects . 1- No harming tissue or causing pain

  22. Biomedical Significance of non-thermal plasma including the following:- Sterilization Vascularization Blood includes antimicrobial By coagulation effect against ROS & NO numerous of includes from microorganisms accelerating of blood Non-thermal plasma include (Bactria, coagulation jet Fungi and virus)

  24. The present study aimed to fulfill two main goals, : 1- Test whether atmospheric non-thermal plasma could have a positive role in promotion and / or acceleration of healing process in diabetic albino rats and Why?. ةباصملا ناذرجلا ىف حورجلا مائتلا نم ليجعتلا ىلع هردقلا اهل ةثافنلا امزلببلا له اذاملو ىركسلاءادب 2- Assess the adverse effects of non-thermal plasma treatment on some biochemical and hematological parameters in albino rat. ءادب هباصملا ناذرجلا ىف ةيويحلا فئاظولا ىلع ةيبناج راثا اهل ةثافنلا امزلببلا له ىركسلا

  26. ❖ Kidney diseases are common health problem worldwide. ❖ There are different types of kidney diseases: Plasma jet device Animal model

  27. Atmospheric non-thermal plasma jet source (ANPJ): ANPJ device was designed by ANPJ team work (plasma and nuclear fusion department of Nuclear Research Centre, Atomic Energy Authority- Inshas) with cost about (3150 LE). 2-Low costs

  29. Active species generated from plasma jet

  30. 2 - Set up the best conditions for ANPJ topical treatment of diabetic wounds in rats. • The feed Gas was air • Flow rate 12 L / min. • 2 cm distance from the outer electrode. • Plasma dose was (0.17 J/cm 2 ) meanwhile, the destructive dose over(135J/cm 2 )

  31. Animals 40 male albino rats were used in this study. The animals were housed as 1 rat per cage under standard laboratory conditions.

  32. 3- Induction of Type 2 Diabetes: • Type 2 diabetes was induced in rats by intraperitoneal injection of freshly prepared stretozotocin (STZ) (60 mg / kg) 15 minutes after intraperitoneal administration of 140 mg / kg nicotinamide. • Three days following STZ injection, animals with blood glucose level above 250 mg / dL were used for the study.

  33. Experimental design 40 male albino rats were shaved & divided into the following groups Group(2): without Group(1): treatment ( wounded normal and without any treatment) Group (3): Diabetic Group (4): Diabetic rats rats treated by plasma treated with antibiotic (2 g) dose (0.17 J/cm 2 ) . 3day \ weak for 21 days. 3 day /weak for 21 days.

  34. Wound Surgery: After confirming the induction of diabetes, 10 animals per group were used to create wounds. About 250 mm 2 full thickness open excision wound was made on the back of the rat.

  35. Topical Measured Parameters effects Systemic Side effects effects Homeostasis, Sterilizations Rate of hair and percent VEGF, Growth of wound CBC, PT, PTT Protein S,C healing Kidney, Fibrinogen Liver and total functions and T3 Antioxidant Mortality&Behavior capacity • 35

  37. Hemostasis • Plasma exposure for 5 sec.

  38. Fungi growth normal G1 no any growth of bacteria • Fungi and Bactria growth Without any treatment group G2

  39. • No growth of both fungi and bacteria • Plasma treated group G3 3-Sterilization Without antibiotic Growth of both fungi and bacteria Antibiotic treated group G4

  40. Day (0) without treatment plasma treatment Day(0) • Antibiotic treatment G2 G3 G4 +ve plasma Fus.

  41. Day (5) without treatment plasma treatment • 9 % • 70% Day(5) • Antibiotic • 25% treatment G2 G3 G4 +ve plasma Fus.

  42. Day (10 ) without treatment plasma treatment • 97% • 50% Day(10) • Antibiotic • 60% treatment G2 G3 G4 +ve plasma Fus.

  43. Day (15) without treatment plasma treatment • • 67% Day(15) • 99.8% 4-Healing at Short time • Antibiotic • 80% treatment G2 G3 G4 +ve plasma Fus.

  44. Day(21) without treatment plasma treatment Day(21) • Antibiotic treatment G2 G3 G4 +ve plasma fuside

  45. normal VEGF level in all groups 0.8 Without 0.7 level of VEGF G1 treat . 0.6 G2 0.5 G3 0.4 0.3 G4 0.2 Plasma 0.1 treat. 0 G1 G2 G3 G4 Groups Antibiotic treat . Total antioxidant capacity in all groups 0.8 0.7 G1 0.6 level of TAC G2 0.5 G3 0.4 G4 0.3 0.2 0.1 0 G1 G2 G3 G4 Groups

  46. Fibrinogen level in all groups G1 490 G2 normal 480 G3 470 Fibrongen level G4 460 Without 450 treat . 440 430 420 410 Plasma 400 treat. G1 G2 G3 G4 Groups Antibiotic Level of Protein S and C in studied groups treat . 200 Protein( S &C ) 180 percent "%" 160 G1 140 120 G2 100 80 60 G3 40 20 G6 0 Protein S Protein C

  47. Hematlogical paramters in all studied groups 16 normal 14 G1 Paramters levels 12 Without 10 G2 treat . 8 G3 6 Plasma 4 G6 treat. 2 Antibiotic 0 treat . HB % RBc WBC Plt. level in all groups 490 480 470 G1 Plt. level 460 G2 450 440 G3 430 G4 420 410 400 G1 G2 G3 G4 Groups

  48. . PT & PTT time in studied groups normal 25 PT & PTT time " Sec." 20 Without G1 treat . 15 G2 10 G3 Plasma 5 G6 treat. 0 PT PTT Antibiotic treat .

  49. urea conc. in all groups G1 normal 1.6 G2 1.4 G3 urea conc.Axis Title 1.2 G4 Without 1 treat . 0.8 0.6 0.4 0.2 Plasma 0 treat. G1 G2 G3 G4 Groups Antibiotic treat . Creat. conc in all groupsChart Title G1 1.6 creat. conc in all groups Axis Title G2 1.4 G3 1.2 G4 1 0.8 0.6 0.4 0.2 0 G1 G2 G3 G4 Groups

Recommend

More recommend