Asya Rolls, Damien Colas, Antoine Adamantidis, Matt Carter, Tope - PDF document

3/11/2013 Optogenetic Manipulation of Sleep Continuity Impairs Memory Consolidation Asya Rolls, Damien Colas, Antoine Adamantidis, Matt Carter, Tope Lanre-Amos, H Craig Heller, and Luis de Lecea Presented by Brian Jacobs and Sam Doernberg BIONB 4110 Undergraduate Journal Club in Neuroscience Dr. Craig Heller The Journal • PNAS PhD Yale University (1970) • IF=9.861 Professor of Biology and Physiology-Stanford University • Very Popular Journal With relatively high impact factor Dr. Asya Rolls Luis De-Lecea • PhD in Molecular Biology University of • PhD The Technion-Israel (2002) Barcelona (1991) • Professor at Stanford Medical School • Professor at Stanford University • PhD in psychiatry • Psychiatry and Behavior-Sleep Center • Biochemistry 1

3/11/2013 The Rest of the Staff Memory Creation in • Antoine Adamantidis PhD(post doc) the Brain • Damien Colas PhD (post doc) • Matt Carter-PhD (post doc) • Tope Lanre-Amos (Med Student) • Duration • Drugs What are the inherent problems with sleep studies? • Continuity • Intensity • Stress • Flexibility Optogenetics • Recombinant DNA What is the theorized • Sensitivity to light role of HCRT • Allows for control neurons in sleep? 2

3/11/2013 Intro Methods • Difficult to control • Difference between NREM and REM • Optogenetic Stimulation • Inherent problems with external stimuli • Hypocretin/orexin neurons • Channel Rhodopsin stimulation • Channel Rhodopsin • NOR Test Results – Results (contd.) Results (contd.) 3

3/11/2013 Results (Contd.) Results (contd.) Results (contd.) Discussion • From an Evolutionary standpoint why are the findings of this study intriguing/confusing? • Continuity vs. memory performance • Duration vs. memory performance • What could this mean for certain memory impairments and diseases? 4