Brain Blood vessels Lungs Heart Pancreas Liver Kidney Cartilage Bone Muscle 1
Scanning electron micrograph of Mycobacterium tuberculosis 2
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Sarah Stanley Deb Hung 4
1.7 million deaths/year Tuberculosis 9.2 million new cases/year Estimated new TB cases per 100,000 population WHO report, 2008 5
Search for tuberculosis treatments Without drug With drug blue = human cells green = tuberculosis bacteria 6
Put bacteria and cells in individual wells of multi-well plates Add thousands of test chemicals, each chemical in a different well Automated microscope 7
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Rifampicin - positive control 15 Number of bacteria : 211 Number of human cells : Average roundness of human cells (choose a score = 10,9,8,7,6,5,4,3,2,1): 5 10 = round irregular = 1 9
Computerized image analysis Find cells Find bacteria Count the number of bacteria per cell 10
www.CellProfiler.org Cell measurements 11
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Remaining slides go along with the teacher ’ s video guide 13
www.CellProfiler.org 14
www.Scratch.mit.edu 15
See the written teacher ’ s guide for: Sources for materials for a bacterial growth experiment Websites with lesson plans for testing everyday samples for bacterial growth 16
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