01 02 3456 78398 0 09 01 02 3456 78398
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!"#$%&' +,-./,.-01+,-./,.-02/3456-78398 - PowerPoint PPT Presentation

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  1. R29..17139,1)6+)7+,0/+.,4-3,-492+.B93/ $%&'()'*(#+'&(#,- B346792>0A797,7392;YB2>346792/D6887A7/6,739 +U[@ U;NV B;W) +6402UD688 +6402B34679 B6GI20,26D<2O"!!]R2 ;2-1#9,$049#,4#> I2 ;% 255"$%! '()"!* !"#$%#!&

  2. ?BB2139,1)6+I")N8)>J r8023A2,C02B';D72>6,62,32>0A790M @-3,0792B3467982 @-3,0792E-6P409,8 '()"!* !"#$%#!&

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  4. P/..+.1G6171396, e;f%geOfQgeQf_g e_f'ge'fLgeLfKgeKf^g Z04/.0)2N+h;f%+"9>>),0+W$S92-/ PB e;f%fOfQg ^ K e'fLfKf^g O Q _ ' L e_f'fLfKf^g ; % eOfQf_f'fLfKf^g VB e922g ")4/+.1G6171396, '()"!* !"#$%#!&

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  13. Z04/.0)2N.+h;f%+ " +"?""5Z(+W$Y92-/+IPBf+VBJ (1G0+W$S92-/.+ " +7/*/4+B94,1,1)6. Z04/.0)2N+hO+ " +R2-.,/4+P/S/2+I $# J P)*+i#+32-.,/4+S92-/+ " +7/*/4+B94,1,1)6. ?BB21/N+,)+,0/+jQ_f&&&+30916.+16+W8!+ I8/3+%&&OJ '()"!* !"#$%#!&

  14. $5CC "g!1]"g %<Lk O<; $5CC "Hg1]H] O<^k Q<L $C6>' H"1$$$ $FJ0 ]$!1&$! R)6./4S9,1)6 F/.1N-/+6->D/4 ;B00+ I5X:N)4/N-3,9./+74)>+ .#*/0%1%)(#'23/%4*#5*)# J '()"!* !"#$%#!&

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  16. $5CC $1]Hl O<Kk ;&<O $5CC $1]$* O<Kk ;L<% $A06U "1&*& $Dg>' "1]*& R)6./4S9,1)6 F/.1N-/+6->D/4 ;B00+ I5X:N)4/N-3,9./+74)>+ .#*/0%1%)(#'23/%4*#5*)# J '()"!* !"#$%#!&

  17. ;B00+ I5X:N)4/N-3,9./+74)>+ .#*/0%1%)(#'23/%4*#5*)# J '()"!* !"#$%#!&

  18. B3467926887P9409,2 A-3428,-./,.-0 '()"!* !"#$%#!&

  19. %;'O+30916.+74)>+#.29>+ *&'(3! +;^^_+ " +_'^ _'^ 2 T)6$4/N-6N96, %;'O ++++++ l%k+mm+lO&99+N177< 81S1N/+496N)>2:+16,)+,*)+ ,*)+./,. F/>)S/+)7+163)>B2/,/+)4+)D.)2/,/+/6,41/. < [#T?P` Z491616G+./,+ " +%Q%+30916. Z/.,16G+./,+ " +%OQ+30916. '()"!* !"#$%#!&

  20. Z04/.0)2N.+h;f%+ " +"?""5Z(+W$Y92-/+IPBf+VBJ (1G0+W$S92-/.+ " +7/*/4+B94,1,1)6. Z04/.0)2N+hO+ " +R2-.,/4+P/S/2+I $# J P)*+i#+32-.,/4+S92-/+ " +7/*/4+B94,1,1)6. '()"!* !"#$%#!&

  21. F+n+Y)2->/c?=? 8)>916+ " +>9XIlN1.,+7IFJoJ '()"!* !"#$%#!&

  22. s>78,2,32AO=Rt 1!<$$ 1!<$! 1!<!l 1!<!g _$Q' ;$KQ QL$KQ K_$;^% ;$%O^ ;^O$%O^ K_$%O^ ;N04p+ IN10:N4)B,/41N16/+4/N-3,9./+J '()"!* !"#$%#!&

  23. T)6$4/N-6N96,+%OQ+30916. b2/3--0/,25-0>7/,7398 '()"!* !"#$%#!&

  24. E-6P409,826887P9409,2 A-3428,-./,.-0 =050,7,7398 +T65579P U345D0409,6-7,708 '()"!* !"#$%#!&

  25. F1D).)>92+B4),/16+='+I;41.J++ "01 '' " + 6*44%0%78)'98,*'0%1(8) !<& !<]% !<] !<"% !<" !<$% !<$ !<!% ;//^? 2$H<gb27><2"<]o ! $ % g $] $H "$ "% "g ]] ]H &$ &% &g %] %H *$ *% *g H] HH l$ l% lg g] !<*1!<H !<%1!<* !<&1!<% !<]1!<& !<"1!<] !<$1!<" !1!<$ ',1>! 2$$<$b27><2"<*o '()"!* !"#$%#!&

  26. R:,)304)>/+R+W/4)X1N9./+I%3:BJ++ (33: "2 ' " + ;;.:38,*'0%1(8) !<& !<]% !<] !<"% !<" !<$% !<$ !<!% ! $ "" &] *& l% $!* $"H $&l $*g $g! "$$ "]" "%] "H& %^cOQ++%<^k %LcO_++O<%k '()"!* !"#$%#!&

  27. '6-96802B346791+T65579P '()"!* !"#$%#!&

  28. /CG43,-G5879279C7F7,3-2" $1&!2u2&$1*& $1]H2u2]l1*& 12Y07-62^WI2B6S782'I2W6>.-90-2;XI2'./JD02;(I2X6G2X@I2E0-8C,2;=<2$gg*<2N3T6->82,C02/345D0,028,-./,.-6D2/C6-6/,0-7Q6,73923A2625-3,0792 A3D>79P256,CT6Gq2,C028,-./,.-0823A2,C02>096,.-0>I2,-6987,739269>296,7S028,6,082A3-2,C026883/76,739#A3D>79P23A2,T32/345D0409,6-G2A-6P409,823A2 /D06S0>2/CG43,-G5879279C7F7,3-2"<2B7-0/,20S7>09/02A3-2629./D06,7391/39>0986,739240/C69784<2 J-67$C#> 2 K q$lg1"!l<2 12W6>.-90-2;XI2),QC6J72W+I2>02@-6,2XXI2E0-8C,2;=<2$ggH<2U345D0409,6,73923A2505,7>02A-6P409,823A2,C02879PD02>3467925-3,0792/CG43,-G58792 79C7F7,3-2"<2 ?$&-6$%9-6 2 (LM q]$H1]"g< '()"!* !"#$%#!&

  29. =/]-/63/+.B93/+<S.<++=,4-3,-4/+.B93/ Z0/+W8!+1.+9+3)S/416G+./,+)7+.>922+B4),/16+.,4-3,-4/.< f7C6-62BI2+J3D97/J2^<2O"!!]R2 ?$&-6$%9-6 <2 OOQ+ 55Hg]2 '()"!* !"#$%#!&

  30. =/]-/63/+.B93/+<S.<++=,4-3,-4/+.B93/ B6,62A-342B';D7 S"<!2 T7,C246_74.42806-/C2856/023A2"!!!2/C6798 '()"!* !"#$%#!&

  31. =/]-/63/+.B93/+<S.<++=,4-3,-4/+.B93/ "!1"%b b % " 1 ! " "!1"%b '()"!* !"#$%#!&

  32. !"#$%&' )*+,-*,+./)*+,-*,+.0-1234+56176 ).8,.7-./)*+,-*,+.0-1234+56176 &4+-09:0&4+*5/;.712 96656*47*09<=,7-*0>+1?.661+ @.34+*2.7*01?0%513A4+24-.,*5-4B0)-5.7-.6 C.D+,4+E0FG *A H0"!!$ %&'"!( !"#"!#!$

  33. ()*+,)+-./+,01.+2/3,-4/. 96I02.J K4-A0<4E0L1.60B5I.M %465-057*+1<,-*517 NA.1+E0O+.3+.6.7*4*517/6-1+57L/13*525P4*517Q 9R45B4DB.03+1L+426 NA.5+0+.6,B*6 KS423B.6 ).-17<0<4E0T.0<56-,660*A.04665L72.7*0?1+0*A.0-B466 !"#$%&'()*$+#,-.#/$0123413256$5,7$83,419-,56$5,,-1519-,/ %&'"!( !"#"!#!$

  34. 5-,216/+)7+,0/+2/3,-4/. @4E0F:0 )*+,-*,+./)*+,-*,+.0-1234+56176 )12.0B11I04*0<4*4D46.601?03+1*.5706*+,-*,+.0-B4665?5-4*517 @4E0": ).8,.7-./)*+,-*,+.0-1234+5617 @.6-+53*51701?0*A.0-B46604665L72.7* %&'"!( !"#"!#!$

  35. 89:+%;+<-==94: ).8,.7-./)*+,-*,+.0-1234+56176 %.?1+.0T.06*4+*M )12.0*A.1+EM @124570D1,7<4+5.6 )*+,-*,+4B03+.<5-*51760?+1206.8,.7-.M >)'/>;K@0O))K03+.<5-*517Q )9U'VW0OL4303.74B*5.6047<06,D6*5*,*517024*+5-.6Q 2V.7NA+.4<.+0O))K03+.<5-*517047<04B5L72.7*#31*.7*54B06-1+.6Q C,L,.0OL4303.74B*5.6047<06,D6*5*,*517024*+5-.6Q X@/Y,+E0O460402.*406.+R.+0.S423B.Q ZA4*0T1+I6M K[906.+R.+0OK[9/NA+.4<57LQ %&'"!( !"#"!#!$

  36. 8>?+% @A)2B+9..1C6=/6,D %&'"!( !"#"!#!$

  37. </E-/63/+.F93/+;G.;++<,4-3,-4/+.F93/ "!/"\] ] \ " / ! " "!/"\] %&'"!( !"#"!#!$

  38. H/6/492+)G/4G1/*+IJ04/9B16CK &4*-A.606.8,.7-.60*10X@06*+,-*,+.6 ;.8,5+.60406-1+57L0?,7-*5170*10466.60*A.0?5*01?0406.8,.7-.0 *1040L5R.70?1B< )-1+57L0?,7-*51760<.+5.<0?+120I71T706*+,-*,+.6047<057-B,<.0 4*120-17*4-*047<061BR4*5170*.+260.R4B,4*.<057040345+T56.0 ?46A517 &4E057-B,<.06.-17<4+E06*+,-*,+.0*.+26H02,B*53B.0 4B5L72.7*6M NA+.4<57L06.+R.+604R45B4DB.0,657L06.R.+4B0<5??.+.7*0433+14-A.6 C1B<0+.-1L75*51706.+R.+04*0'23.+54B0^1BB.L.H0_`00000000000000000000 0000 A**3a##TTT:6DL:D51:5-:4-:,I#bX<3662# >+1*.57>+.<5-*06.+R.+04*0K&%U0000000000000000000000000000000000000 A**3a##TTT:.2DB/A.5<.BD.+L:<.#3+.<5-*3+1*.57#3+.<5-*3+1*.57:A*2B >+1*.5706.8,.7-./6*+,-*,+.0*A+.4<57L04*0W^%'00 A**3a## TTT:7-D5:7B2:75A:L1R#)*+,-*,+.#;K)K9;^c#*A+.4<57L:6A*2B %&'"!( !"#"!#!$

  39. J/=F29,/+3)=F941.)6+=/,0)B. _6.60X@0d*.23B4*.6e0?1+06.4+-A57L06*+,-*,+4B0<4*4D46.6 4-*5R.065*.01+0D57<57L065*.0*.23B4*.60L.7.+4*.<0*10+.?B.-*0?,7-*5174BBE0 5231+*47*06*+,-*,+4B065L74*,+.6 9R45B4DB.061?*T4+.#6.+R.+6 N.23B4*.0).4+-A047<0),3.+3165*5170ONK))QH0NA1+7*170V+1,3000000000 A**3a##TTT:D51-A.2:,-B:4-:,I#D62#>;f^9N#>;f^9N:A*2B00000000000000 00000000 Z4BB4-.09^g0%1+I4I1*50Wg0NA1+7*170Y&:0OFGGhQ0 :2-1#9,$049#,4#$ ' 033"X!i dC,PPE0C,7-*5174B0C1+26e0H0)I1B75-I0/0-122.+-54B04R45B4D5B5*E00000000000 0 C.*+1TH0Y6047<0)I1B75-IH0Y0OFGGiQ00 ;/$&-/$%9-6 0 %LM 033G$G )34*54B09++47L.2.7*601?0)5<./-A457047<0&457/-A4570O)>9)&QH0 `B.ET.L*H0_75R:01?0_3364B40000000000000000000000000000000000000000000000000000 A**3a##31+*+4E:D2-:,,:6.#-L5/D57#<.7756#63462:3B000000000000000000000000000000 0 `B.ET.L*0VY0OFGGGQ:0 ;/$&-6/$%9-6/ 0 %LN 033Fiih 00000000000000000000000000 %&'"!( !"#"!#!$

  40. Sequence-Structure alignments >.+96:+),0/4+O1)167)4=9,13.+F4)O2/=P $+Q/F4/./6,9,1)6 $+<3)416C $+5F,1=1R/4 %&'"!( !"#"!#!$

  41. S=F141392+/6/4C:+7-63,1)6.+IT"AK '<.4a0 /6/4C:+2/9B.+,)+.,4-3,-4/U+,0-.+1,+.0)-2B+O/+ F)..1O2/+,)+167/4+/6/4C:+74)=+=96:+V6)*6+ .,4-3,-4/. N10D.0,6.<057a021<.B0+.?57.2.7*047<0466.662.7* >+13.+*5.607..<.<a @..3025752,204*0-1++.-*06*4*.0O74*5R.Q )211*A )523B. NE3.6a ^17*4-*031*.7*54B @56*47-.031*.7*54B6 ),+?4-.031*.7*54B6 %&'"!( !"#"!#!$

  42. >FF4)W1=9,1)6.XY1=1,9,1)6.+16+T"A. @4*4D46.065P.: >&C0R.+6,60K7.+LE0O4<<5*5R.#A5LA.+01+<.+0*.+26Q: ;.?.+.7-.06*4*.: >AE65-4B01+5L57: C57I.B6*.570.*04B:0OFGG\Q0 :2-1#9,<$ %Z H033F$" %&'"!( !"#"!#!$

  43. Q/F4/./6,9,1)6 </E-/63/X<,4-3,-4/. < 5 !"#$%&$'()' !"#$%&$'()' *+,-./..0-1002033.43--5 *+,-./..0-1002033.43--5 >+524+E06.8,.7-. @56*47-.0634-. 9BB04*126047<0-11+<574*.6 ^ ! ;.<,-.<04*1260+.3+.6.7*4*517 ).-17<4+E0)*+,-*,+. 00000000009--.665DB.06,+?4-. %&'"!( !"#"!#!$

  44. <3)416C <,9,1.,1392+T),/6,192P+16.F149,1)6 " # >% $ ? " # " # >+++\+++ +++\+++! !++++++++++++++++++ ++++++++++++++++++> >! ! > % > % " # >% & $ ' $ ' @ A! B7O Q ? A! B7 " # " # % > % % 9 9 % 9 % % N474I4047<0)A.+4L40OFGh\Q0 :=>0 H0 [%+ 33Xi!" 9:0V1<P5IH0OFGG(Q0 01234132# 0 MN 033X(X %&'"!( !"#"!#!$

  45. <3)416C <,9,1.,1392+T),/6,192P+16,/493,1)6+,:F/. W.,*+4B057*.+4-*5176 cE<+13A1D5-057*.+4-*5176 ^1234-*057*.+4-*5176 )3.-5?5-057*.+4-*5176 9:0V1<P5IH0OFGG(Q0 01234132# 0 MN 033X(X %&'"!( !"#"!#!$

  46. <3)416C <,9,1.,1392+T),/6,192P+4/7/4/63/+.,9,/ NA.1+E01?06523B.0B58,5<60"7<0.<5*5170Y>0c476.7047<0';0&-@174B<H009-4<.25-0>+.66: %&'"!( !"#"!#!$

  47. <3)416C <,9,1.,1392+T),/6,192P+(:B4)C/6+!)6B. U17L0+47L.0?+..0.7.+LE C+..0.7.+LE01?0*A.03+1*.570 D4-ID17.0AE<+1L.70D17< W0j0j0j0f0-1235B.<0?+12040 <4*4D46.01?00"iG0k/+4E06*+,-*,+.6 ) * 2 2 $ ( ' O Q 2 O Q 9B =C 9B )A1+*0+47L.0?+..0.7.+LE ) O Q 2 + $ O Q 2 =C ) " =C + , + E $ ' O Q 2 D! B7 O Q 2 =C =C )533B0OFGG(Q:0 ;&% 0 %'& 033($$ %&'"!( !"#"!#!$

  48. <3)416C <,9,1.,1392+T),/6,192P+81.,963/+T),/6,192. U17L0+47L.0?+..0.7.+LE )A1+*0+47L.0?+..0.7.+LE )533B0OFGGXQ:0 ;F>& 0 [ 033$hX %&'"!( !"#"!#!$

  49. <3)416C Q9*+.3)4/.+)7+96+921C6=/6,+ < 5 925714-5<06,D6*5*,*5176 @56*47-.0634-. ).-17<4+E0)*+,-*,+.0OcH%H^Q 9--.665DB.06,+?4-.0O%H90l]mQ %&'"!( !"#"!#!$

  50. <3)416C + <1C61713963/+)7+96+921C6=/6,+I.3)4/K >+1D4D5B5*E0*A4*0*A.013*524B04B5L72.7*01?0*T10+47<120 6.8,.7-.6#6*+,-*,+.601?0*A.0642.0B.7L*A047<0-123165*5170460*A.0 4B5L7.<06.8,.7-.6#6*+,-*,+.60A4R.04*0B.46*0460L11<0406-1+.0460*A.0 .R4B,4*.<04B5L72.7*: )12.*52.60 K235+5-4B 433+1S524*.< DE0n/6-1+.0O71+24B <56*+5D,*517Q: - . / 0O / < Q # $ : < O Q0 0 974BE*5- + , $ ' - . / 0O / Q < 0 F .S3 / # : < O G Q0 `4+B57047<09B*6-A,BH0FGG!0 :=>0 0 L[ H033""($ %&'"!( !"#"!#!$

  51. <3)416C + <1C61713963/+)7+96+921C6=/6,+I.3)4/K K7.+LE0n/6-1+.0*A.021<.B0T5*A0+.63.-*0*A.0.7.+LE01?0+47<120 21<.B60O1+0+.6*01?0<.-1E6Q: 0 K + , ' H H /K oK $ . I<4-2# 0 H pKq %&'"!( !"#"!#!$

  52. <3)416C + <1C61713963/+)7+96+921C6=/6,+IO93VC4)-6BK )*+,-*,+4B0634-. ).8,.7-.0634-. !"##$%#&'$(#'('$'&%%)*'+(,"-.%#/0-'"%). .#(%,)'%##-)''-"('"$'&.#$($/%%#&'*+%0"! !.%,'%/)'(#)0'$"$+-*%#"&'$#'%#"-'#&(%(. %.#%)-&('''"",'$'.%/$*(#0$#&"(%)#+'#%-! !)''*%)"$'0##+%/#'"#.$(&-('"$#'%%&(%-., %&'"!( !"#"!#!$

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  73. SG9+</4G/4 PDB SEC CM FR CON Sequences Servers Servers Servers Servers EVA Center + META-PP SEC Evaluation CM Evaluation FR Evaluation CON Evaluation WWW http://cubic.bioc.columbia.edu/eva – http://pipe.rockefeller.edu/~eva – http://montblanc.cnb.uam.es/eva 67789::AC;<A@;<=A@A=DCH;<E@NKC:NOE5P567789::8<8N@G=AFN?NDDNG@NKC:QNOE5P567789::H=>7;DE>A@A>;@CEH@NB:NOE %&'"!( !"#"!#!$

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