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William and Mary iGEM 2014 Calcium http://arthritida.com / http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm

  1. William and Mary iGEM 2014

  2. Calcium http://arthritida.com / http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm http://www.understandinganimalresearch.org.uk/assets/document/37A5997B-D2A9-B867-D7E46F273A86638A/annual-report-for-20.pdf http://openi.nlm.nih.gov/detailedresult.php?img=2689218_1750-1326-4-20-1&req=4 http://www.lifetechnologies.com/order/catalog/product/T3605

  3. The Calcium Kit: Tools for the In Vivo Sensing and Manipulation of Calcium http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf

  4. Motivation Video courtesy of Wendy Herbst at College of William and Mary

  5. Road to Synthetic Biology + Image courtesy of Wendy Herbst at College of William and Mary

  6. Rationale http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf

  7. Project Phases Induction Recording Visualization

  8. Phase I: Induce Ca 2+ Influx Channelrhodopsin: Dimerized, closed state http://www.ebi.ac.uk/pdbe-srv/view/entry/3ug9/quaternary.html

  9. Phase I: Induce Ca 2+ Influx http://www.openoptogenetics.org/index.php?title=Channelrhodopsins

  10. Phase I: Induce Ca 2+ Influx Na + H + Ca 2+

  11. Phase II: Visualize Ca 2+ Presence http://www.lifetechnologies.com/content/dam/LifeTech/migration/en/images/ics- organized/brands/molecular-probes.par.71542.image.400.416.1.g001108-gif.gif

  12. Phase II: Visualize Ca 2+ Presence http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586699/figure/F3/

  13. Phase III: Record Ca 2+ Spikes Ca 2+ Ca 2+ Ca 2+

  14. Completed: Phase I

  15. ChR1 vs CatCh ChR1 CatCh

  16. Begun: Phase II http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full

  17. Goal: Phase III http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell http://2011.igem.org/Team:Groningen

  18. Future Directions: The Calcium Kit http://archiv.aktuelle-wochenschau.de/2013/w51/woche51.html http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell

  19. Future Directions: W&M iGEM

  20. Acknowledgements Undergraduate Science Education Grant to the College of William & Mary William & Mary Dean of Arts & Sciences Dr. Dennis Manos, Vice Provost for Research and Graduate Studies The Charles Center We would like to thank Dr. Peter Hegemann and Dr. James Collins for providing plasmids

  21. Questions?

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