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VI Magnetobiology Cell integration with nickel nanowires Toxicity study Ac field effect on pre-osteoblasts In vitro cell stimulation with strong pulsed fields Planned work Staff, Publications Adriele Prina-Mello

  1. VI Magnetobiology • Cell integration with nickel nanowires • Toxicity study • Ac field effect on pre-osteoblasts • In vitro cell stimulation with strong pulsed fields • Planned work

  2. Staff, Publications • Adriele Prina-Mello Senior postdoc 2006- • Fiona Byrne Postgrad 2006- • Darragh Crotty Postgrad 2005- • Nigel Carrol Experimental Officer • Collaborations: Yuri Volkov (TCD, Clinical Medicine) • Ken Donaldson, Craig Poland (U. Edinburgh) SFI Review 30th January 09 2

  3. Publications • Internalization of ferromagnetic nanowires by different living cells, A. Prina-Mello, Zhu Diao and J. M. D. Coey, Journal of Nanobiotechnology 4:9 1-11 (2006) • High content analysis of the biocompatibility of nickel nanowires. Fiona Byrne, A. Prina-Mello, A. Whelan, B.M. Mohamed, A. davies, Y. Gunko, J.M.D. Coey, Yuri Volkov, Journal of Magnetism and Magnetic Materials , (in press) (2009) • Magnetic-fluorescent nanocomposites for biomedical multitasking S A. Corr, A. O’Byrne, Y. K. Gun’ko, S. Ghosh, D. F. Brougham, S. Mitchell, Y Volkov and A Prina-Mello, Chemical Communications , 4474 - 6 (2006) • The evolution of chemotaxis assays from static models to physiologically relevant platforms S. Toetsch, P. Olwell, A. Prina-Mello and Y. Volkov, Integrative Biology, (in press)(2009) • In vivo EPR for dosimetry, H.M. Swartz, G. Burke and M. Coey, Radiation Measurements 42 SI 1075-1084 (2007) SFI Review 30th January 09 3

  4. MANSE Proposal – Magnetobiology • Investigate the influence of static uniform magnetic fields and field gradients on cellular growth and morphology. • Manipulate and stimulate cells in novel ways, especially using ferromagnetic nanowires and pulsed magnetic fields. MANSE Magnetobiology proposal concept: Living Cells + Magnetic materials + External Field = Manipulation, Stimulation and Sensing SFI Review 30th January 09 4

  8. Cell-NW interaction and Orientation MSCs with internalized nickel nanowires Angular distributions of Ni NW orientation in MSCs in zero field (left). After alignment in a 100 mT applied field for 18 h (right). Cell-NW separation and Angular distributions of MSC+NW morphology in 0 field (left), manipulation and after alignment in a 100 mT applied field for 18 h (right) SFI Review 20 i 09 8

  9. Are the nanowires really internalised? MC3T3-E1 osteoblasts showing internalized nickel nanowire: SEM micrograph Confocal and Z stack confocal micrographs 9

  14. HCS quantitative time response on 20 μ m Ni NW 3 hr 6 hr 24 hr 72 hr NW Concentration NW Concentration

  16. AC field effect on pre-osteoblasts ? Background Literature suggests that low frequency magnetic field can affect osteoblast proliferation, differentiation and morphology (Heermeier et al., Bioelectromagnetics, (1998),19:222-231). Pulsed electromagnetic fields have been used clinically and are thought to enhance tissue regeneration (Shupak et al., Radio Science Bulletin, (2003), 307:9-34). Objective Understand if there is a scientific basis behind the fears concerning the carcinogenicity of 50/60 Hz AC electromagnetic fields, as suggested by some previous studies (Fedrowitz et al., Cancer Res, 62 (2002) 1356-1363; Zhu et al., Am. J. Epidemiol., 158 (2003) 798-806). SFI Review 30th January 09 16

  19. In vitro cell stimulation with pulsed fields Transcranial Magnetic Stimulation (TMS) Important experimental tool in Neuroscience used in clinically for treatment and diagnosis of neurological disorders such as multiple sclerosis and Parkinson’s disease Principle of work in neurons Strong magnetic pulse produced by a flat coil induces a potential difference across the neurons membrane which triggers the neurons firing Objectives To extend the first demonstration of in vitro magnetic stimulation of neurons reported recently by Rotem et al. (2008) To understand the physical and biochemical mechanisms involved in vitro SFI Review 30th January 09 19

  22. Conclusions, future plans • This work is developing valuable interdisciplinary relations within TCD and beyond • Nanowires have potential for cell labelling and manipulation • Demonstration of toxicity based on geometrical size and shape, requiring further examination • Parameters associated with bone cell development and carcinogenesis not affected by weak AC fields • In-vitro pulsed-field stimulation is a new and currently understudied area, which will be developed SFI Review 30th January 09 22

  23. 23 SFI Review 30th January 09

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