6/19/2015 LABORATORY PROCESS Scientist Method Development Sample BecA ‐ ILRI Hub Laboratory Management and Equipment Operations (LMEO) workshop ‐ RUBONA, Rwanda Testing Sample Preparation Instrument TOPIC: LABORATORY INSTRUMENTATION th June 201 DATES: 18 DATE 18 th June 2015 Data Data Analysis Decision Making/Recom Tom Ouma Tom O ma ASPECTS OF INSTRUMENTATION VALUE OF LABORATORY EQUIPMENT For effective laboratory instrumentation to be achieved, the The value of each laboratory equipment is mainly measured based 1. following aspects should be met; on its capability to provide data which is reliable, consistent, timely 1. The laboratory management system should be in place to and affordable. guide the acquisition(including supplier audits), utilization, 2. It is critical that prior to acquiring any equipment, a proper need maintenance & disposal of the instruments. assessment should be carried out to ascertain the demand and 2. The equipment must be properly installed. technical requirements. 3. The equipment must be qualified(IQ ,OQ & PQ) prior to use. 3. It is also important that one considers the long term value 4. Maintenance service contract is highly recommended. addition/benefits against the cost. 5. Calibration schedule should be in place where necessary. 4. It is advisable that the user/manager consult with the equipment Records on complaints, investigation, corrective action & 6. distributors/manufacturers in order to know the current technology internal audits should be available throughout shelf-life. available and their capabilities. 7. Should be capable of enhancing high levels of confidentiality/user level controls & Audit trail features . Networking with other labs in the same category is highly 5. encouraged. 8. There should be effective resource utilization programs. 9. There should be a master list of all the equipment and their 6. Resource mobilization is important prior to procurement. status update. Competent technical evaluation should be carried out on all offers. 7. MAJOR STEPS IN SAMPLE TESTING TECHNIQUES � Sample Preparation � Digestion techniques – � Chromatography � SPE Multiwave digestor, � AAS & ICP (Elemental) � LLE Hydrotherm, � QuEChERS � FTIR Spectro Soxtherm(fat & Lipids, � Digestion -Multiwave � Raman Spectro Dumatherm(Protein & � Sample Seperation � UV-Vis � LC Nitrogen, Kjeldahltherm � GC (metals, COD, Cyanide ) � XRD/EDX/XRF � Compound Detection & � NIR Quantification � PDA � Polarimetry � UV � Refractometry � MS, MS/MS, 1
6/19/2015 Trace Level Residue Analysis Application � Many compounds like pesticides have been discontinued due to health & environmental risks. Their continued use has left heavy contamination & � Chromatographic technique is widely applied in need of remediation. most environmental, drugs and food testing with HPLC and GC being the commonly used. � PAHs are a class of persistent organic pollutants. � HPLC –Carbomates, pesticides, PAHs and � Need for: explossives. Plant & Food protection. 1. � GC- Pesticides, Herbicides, VOCs, SVOs and Food chain safety. 2. PAHs (Polinuclear Aromatic Hydrocarbons. Environmental risk assessment. 3. Crisis response. 4. Economic gain. 5. MULTI-RESIDURE TESTING IN QuEChERS FOOD � roQ Quechers roQ quechers Kits (Phenomenex).pdf SHIMADZU LC-MSMS Requirements for Triple Quads � Sensitivity � Reproducibility � Speed Pharmaceutical Life Science Chemistry Sprayer � Robustness � Usability Q1 Pre ‐ rod Q1 Post ‐ rod Q3 Pre ‐ rod Octopole 1 Qarray Q1 Q3 � Affordable Price Nano Food Safety Molecular Desolvation Technology Imaging Line Detector Skimmer Octopole 2 UFsweeper TM Collision Cell 1 st Vacuum 3 rd : Atmospheric chamber: 100 2 nd : 5 Pa 4 th : 3 x 10 ‐ 3 Pa Pressure 0.1 Pa to 200 Pa 4 Stage Differential Pumping System 40 260 210 RP by a Triple Inlets TMP and a RP L/S Forensic Environmental L/S L/S New Energy Electronics 11 12 2
6/19/2015 APPLICATION MULTI-RESIDURE METHOD Mycotoxins � Neat std mix 138 compounds on LC-MS/MS 8040 � 72 =LC-MS/MS � 66 =GC-MS/MS � LC/MS/MS LOQ <10ppb 72 (100%) � GC/MS/MS LOQ<10ppb 47 (71%), >10ppb 6(9%) � 80% compounds reffered to GC/MS/MS were able to be measured by LC/MS/MS SHIMADZU Method packages provide pre ‐ optimized MRM and HPLC conditions 3
6/19/2015 Compound ID Injection Blood, Urine, Organ QuEChERS Data Output Inten. (x100,000) (x100,000) Zolpidem 6.0 235 Spectrum is acquired at 2.0 CH 3 5.0 N same time CH 3 4.0 N NCH 3 H 3 C 1.0 O 263 3.0 308 221 2.0 0.0 200 250 300 m/z 1.0 MS/MS spectrum (Product Ion Scan) 0.0 Compound ID by Library search 3.0 3.5 4.0 4.5 5.0 5.5 min Chromatogram (MRM) NEXERA SERIES LCMS-8060 Nexera SR Nexera MP LC/MS Nexera Method Scouting features superior frontend HPLC for high sensitivity, sensitivity and offers comprehensive method throughput analysis of stability. development by 96 combinations multiple samples. Nexera TQ and Nexera SQ offers ultra-fast LC/MS analysis by Nexera Quaternary combination of Nexera X2 and UF-MS. is flexible quaternary gradient system. ESTEC LTD INTEGRATED HPLC SYSTEM � Educational Scientific & Technical Company (ESTEC) is a leading Prominence-i & Nexera-i, Series distributor of quality analytical instruments within East Africa region and has been in operation for the last 37 years. During this period, ESTEC has developed into a dependable partner supporting various industries mainly Pharmaceuticals, Research, Beverages, Regulatory, Petroleum, Food, Environmental and educational laboratories. Estec has won the confidence of leading global brands from Europe, Asia and USA by being appointed the sole dealer for Shimadzu (Japan), Anton-paar (Austria), Gerhardt (Germany), Phenomenex (USA) among others. � At Estec we nurture and cultivate a culture of a serving relationship with each customer and this is why we don’t just deliver, we see you through the equipment life-span. We boast of a strong team of technically skilled, experienced and reliable engineers as well as sales staff capable of installing, training, and servicing your instruments. With Estec, you are in safe hands. 4
6/19/2015 1. Application Support 2. Service Support BENEFITS KEY AREAS OF SUPPORT 1. Environmental Labs. � Method packages Residual Pesticides. 2. Food & Agricultural Labs. � NIST Libraries for Vet drugs, Pesticides, Forensic 3. Pharmaceutical Labs. drugs. 4. Petroleum Labs. � User friendly easy to learn & operate LabSolution 5. Beverage Products. software. 6. Forensic Labs. � User Training. 7. Mining Labs. � Locally available skilled Technical support & Spares. 8. Research & Educational Labs � Maintenance Service contract. The End The End Thank you Thank you for your for your valuab valuable le orders & orders & listening listening. Tom Ouma Tom Ouma +254- 254-722 990 22 990 99 996 6 info@esteckenya.com 5
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