Table of Contents Introduction video Team introduction Background - PowerPoint PPT Presentation

I N V E S T I GAT I N G T H E M U LT I D R U G R E S I S TA N C E O F M RSA A N D T H E G E N E S I N VO LV E D

Table of Contents — Introduction video — Team introduction — Background — Safety — Equipment — Process — Results — Conclusions — Further research — Human practice — Sponsors

Introduction Video

Team Introduction — South Korea — Elan Vital — Multi-school biology club — Experimented in the Gachon medical university

Team Members Ye Eun Lee Ji Hoon Kim Young Chan Kim Soo Jung Lee Ji Yong Hong

Yae Sung Mun majoring in bio-nano technology — Team coach — Graduate in Gachon university — Plays the flute

You Jin Hwang badminton — Team instructor — Professor in Gachon university — Loves to play soccer and

MRSA life-threating ones — Methicillin Resistant Staphylococcus aureus — Resistance to beta-lactam antibiotics — Causes a variety of diseases including — Nosocomial infections (Infections in hospitals)

What We Did involved resistance: like finding a common denominator E. coli with drug resistance — Multidrug resistance of MRSA and the genes — Comparing DNA of bacteria that has drug — MRSA with drug resistance & transformed

Transformation protein that the DNA codes for — Insert foreign DNA in cell — DNA codes proteins — The cell will produce the

Safety regulations — WHO Biosafety Levels — E. coli WHO BSL 2 — MRSA WHO BSL 3 — Gachon University of Medicine safety

Equipment f medicine lab — We used lab equipment from the Gachon university o

Process — MRSA + antibiotics — Transform E. coli with surviving MRSA DNA — PCR on the DNA of the surviving bacteria — Gel electrophoresis

MRSA Antibiotic Resistance the antibiotics ampicillin, gentamycin, kanamycin, and tetracycline 50mg/μl for gentamycin, 10mg/μl for kanamycin, and 5mg/μl for tetracycline. — Grow MRSA sample with antibiotics — In the project, we used MRSA from samples #7 and#8, and — LB plates of 1/1000 concentration of stock solution — Stock solutions of concentrations 100mg/μl for ampicillin,

MRSA Antibiotic Resistance Results

E. Coli Antibiotic Resistance transformed — Competent E. coli — Control group for transformation — Competent E. coli are designed to be easily

E. Coli Antibiotic Resistance Results

Transformation confirmed to not have antibiotic resistance) with MRSA that developed drug resistance — Transform E. coli with DNA from MRSA — Extract DNA from MRSA though miniprep — In this case, we will transform E. coli (which was — We want E. coli to develop drug resistance

Transformation Results

PCR reaction runtests such as gel electrophoresis — Polymerase chain — Amplifies DNA — Makes it easier to

PCR Results (Amplification)

Gel Electrophoresis used to analyze DN A comparisons — Gel electrophoresis is — Good for

Gel Electrophoresis the DNA of the MRSA and E. coli with antibiotic resistance in both types of bacteria by comparing gel electrophoresis — We can use gel electrophoresis to compare — We can find the section of DNA that is shared

Gel Electrophoresis Results

Conclusion resistant for multidrug resistance — Successfully transformed E. coli to be drug — Similar bands in gel electrophoresis that could code — We would like to sequence the similar bands

Future Research of MRSA — Sequence DNA sections from gel electrophoresis — Understanding the genes of drug resistance — Finding alternate antibiotics — Finding chemicals to counteract drug resistance

Human Practices pamphlets, hanged up posters, and gave speeches about MRSA — To raise public awareness on MRSA, we handedout

Sponsors — We informed many companies of out project — Some of them decided to help us out. — These are our sponsors 0

Thank you