Results from the Sorcerer II Global Ocean Sampling Expedition
The Sorcerer I I Expedition Global Ocean Sam pling Route www.sorcerer2expedition.org
Sam ple Fractionation
JTC Sequencer Lab Capacity: 240,000 sequences/day or 80 million lanes/year at 24 runs per day
Prelim inary Analysis: Nova Scotia through Galapagos
Nova Scotia through Galapagos Islands W ater Tem perature:
Clustering of GOS sites: Reads incorporated into significant scaffolds Cold Water Samples Warm Water Samples 5 4 6 2 3 8 7 9 10 0 19 17 16 15 14 23 34 29 27 30 21 22 31 25 1
The Biodiversity of Each New Region is Different Flamingo Pond (Galapagos) Flamingo Pond (Galapagos) Panama Canal Panama Canal Central America Central America Galapagos Galapagos Eastern U.S. Eastern U.S. Seaboard Seaboard Sargasso Sea and Sargasso Sea and Yucatan Channel, Mexico Yucatan Channel, Mexico North Atlantic North Atlantic
Proteorhodopsin Multiple Alignment 97 GI proteins 2350 VI proteins 22 pages total
Proteorhodopsins Vary by Region
Highly similar sequence Group of related prochlorococcus strains Highly conserved protein sequences � Sequenced Prochlorococcus genome �
GOS protein analysis 6 5 4 3 2 1 0 GOS Known proteins (NCBI+TGI+ENS) GOS nearly doubles the number of proteins
Num ber of Protein Fam ilies on Earth
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