Remotely Connected Electric Field Generator Timothy Dee, Remotely Connected Electric Field Justin Long, Brandon McDonnell Generator Dielectrophoresis Project Overview for Particle Separation in a Fluid Initial Implementation Design Team May1612 Problems Intermediate Implementation 1 Design Implementation and Problems Intermediate Implementation 2 Design Implementation Problems Final Design Hardware Components Software Components Timothy Dee, Current State Justin Long, Questions Brandon McDonnell Iowa State University 1.1
Remotely Connected Dielectrophoresis (DEP) Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview Initial Implementation • A dielectric particle in a Design Problems non uniform electric field Intermediate experiences a force Implementation 1 Design • Different potential fields Implementation and Problems and frequencies has an Intermediate Implementation 2 effect on the net force Design Implementation • First studied in 1950s by Problems Herbert Pohl Final Design Hardware Components Software Components Current State Questions 1.2
Remotely Connected Real World Application Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Dielectrophoresis Project Overview Initial Implementation • Recently revived due to the ability to manipulate Design Problems micro-particles and cells. Intermediate Implementation 1 • Potential to separate particles in spinal fluid Design Implementation and • Act as filter Problems Intermediate • Research in separating cancerous cells from healthy cells Implementation 2 Design • Separate platelets from whole blood Implementation Problems • Separate red and white blood cells Final Design Hardware Components • Separate Strains of bacteria and viruses from living cells Software Components Current State Questions 1.3
Remotely Connected Project Description Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview • A system to aid in of DEP research Initial Implementation Design • Allow for quicker setup times Problems Intermediate • Control Voltage and Frequency via the web Implementation 1 Design • 1 to 60 VPP Implementation and Problems • 10k to 1Mhz Intermediate Implementation 2 • Hold output for long time periods Design Implementation • Small Form Factor Problems • Easy to use Final Design Hardware Components • Plug and play Software Components Current State Questions 1.4
Remotely Connected Project Structure Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview Initial Implementation • Raspberry Pi Design Problems • Web Interface Intermediate Implementation 1 • Web Server Design Implementation and • Frequency Control Problems Intermediate Solution Implementation 2 Design • Voltage Control Solution Implementation Problems Final Design Hardware Components Software Components Current State Questions 1.5
Remotely Connected Initial Implementation Electric Field Generator Timothy Dee, Justin Long, • Raspberry Pi Brandon McDonnell • Host web server Dielectrophoresis • Remote manipulation of circuit output Project Overview • Web interface can provide additional functionality Initial Implementation • GPIO pins input to circuit Design Problems • Circuit Output Intermediate Implementation 1 • Frequency generated by GPIO pin Design • GPIO waveform integrated to get sine wave Implementation and Problems • Sine wave amplified to form output Intermediate Implementation 2 Design Implementation Problems Amplifier preAmplifier Circuit for Generated Circuit Final Design 60Vpp Electric Field GPIO Hardware Components Software Components Integrator circuit to Raspberry Pi convert from Current State square wave to sine wave Questions Web Server 1.6
Remotely Connected Concerns Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview Initial Implementation • Raspberry Pi Design Problems • Complexity of programming Intermediate • GPIO pins may only be turned on and off Implementation 1 Design • On-off mechanism must be used to generate waveform Implementation and Problems • Current load Intermediate • Circuit Output Implementation 2 Design • Complexity of construction Implementation Problems • No guarantees about cleanliness of GPIO pin waveform Final Design • High risk of failure Hardware Components Software Components Current State Questions 1.7
Remotely Connected Minigen Function Generator Electric Field Generator • SPI communications Timothy Dee, • Small form factor Justin Long, Brandon McDonnell • Output programmable frequency • Produces 1 Khz to 4 Mhz waveforms Dielectrophoresis Project Overview Initial Implementation Design Problems Intermediate Implementation 1 Design Implementation and Problems Intermediate Implementation 2 Design Implementation Problems Final Design Hardware Components Software Components Current State Questions 1.8
Remotely Connected Intermediate Design Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview Initial Implementation Design Problems • Raspberry Pi controls Integrated circuit components Intermediate Implementation 1 • Minigen used to produce frequency Design • Digital Potentiometers Implementation and Problems • SPI communications Intermediate Implementation 2 • Vary resistance to control amplifier Design Implementation • Amplifier controls voltage output from circuit Problems Final Design Hardware Components Software Components Current State Questions 1.9
Remotely Connected Digital Potentiometer Amplifier Circuit Electric Field Generator Properties Timothy Dee, Justin Long, Brandon McDonnell • Utilizes digital potentiometer as feedback resistor Dielectrophoresis V out = − R F R IN ∗ Minigen SIGNAL Project Overview Initial Implementation Design Problems Intermediate Implementation 1 Design Implementation and Problems Intermediate Implementation 2 Design Implementation Problems Final Design Hardware Components Software Components Current State Problems Questions • Distortion of signal • Very low resistance with AC signal 1.10
Remotely Connected MOSFET Amplifier Electric Field Generator Timothy Dee, Justin Long, Properties Brandon McDonnell • Utilizes digital pot in a different way Dielectrophoresis Project Overview • Amplification utilizes transistor Initial Implementation Design Problems Intermediate • Suggested by Implementation 1 Design Minnetronix Implementation and Problems • Distortion of signal Intermediate Implementation 2 remains Design • Concluded digital Implementation Problems potentiometer is source Final Design Hardware Components of problem Software Components Current State Questions 1.11
Remotely Connected Redesign Amplifier Electric Field Generator Timothy Dee, Justin Long, Idea Overview Brandon McDonnell • Previous problems stem from voltage modification Dielectrophoresis Project Overview solutions Initial Implementation • Solution: Use integrated circuit component to modify Design Problems voltage Intermediate Implementation 1 Design Implementation and Amplifier Properties Problems Intermediate Implementation 2 • Three stages of amplification Design Implementation • One PGA and two stages with constant gain Problems Final Design • 20 V pp per stage Hardware Components • Summing amplifier sums stages Software Components • PGA achieves 8 steps within one stage Current State • Switches increase output by 20 V pp Questions • Use transistors as switches flipped using GPIO pins 1.12
Remotely Connected Configuration Electric Field Generator Programmable Gain Amplifier(PGA) Timothy Dee, Justin Long, Brandon McDonnell • Three pins encode gain Dielectrophoresis • 8 Gain Options from 0 to 7 Project Overview Initial Implementation Design 3.3V Problems Ground Intermediate Implementation 1 Design SPI Minigen Implementation and Interface Problems Signal Generator Intermediate Implementation 2 Design Implementation SPI Interface Problems Raspberry Pi Programmable Final Design Hardware Components Gain Amplifier Software Components Current State Questions Switch Circuit Switch Circuit 1.13
Remotely Connected Layout Electric Field Generator Timothy Dee, Justin Long, Brandon McDonnell Dielectrophoresis Project Overview Initial Implementation Design Problems Intermediate Implementation 1 Design Implementation and Problems Intermediate Implementation 2 Design Implementation Problems Final Design Hardware Components Connections Description Software Components Current State • Raspberry Pi connected to components Questions • Output of Minigen goes to input of PGA • All three stages input to summing amplifier 1.14
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