Project: Blue Ivy iGEM Universitas Indonesia ? Tuberculosis? - PowerPoint PPT Presentation

Project: Blue Ivy iGEM Universitas Indonesia

?

Tuberculosis?

Worldwide Infection, 2011 9 4 million million Source: http://www.cdc.gov/tb/statistics/ http://www.who.int/mediacentre/factsheets/fs211/en/

Worldwide Infection, 2011

Is it that hard to erradicate Tuberculosis?

Present Detection Method

Our System

Our Target: Antigen 85  Sputum  Blood serum  Urine Ag Ag Ag Ag Ag Ag Ag Ag 85 85 85 85 85 85 85 85

Our Device  Specific Detector Single Chain Variable Fragment of Antibody  Flexible Linker  Split Reporter Beta-galactosidase

Antigen 85

Why β -Galactosidase?  Well characterized  Visible activity  Cheaper substrate  Longer signal

Parts Characterization Enzimatic Activity Assay Incubation time (hours)

Parts Characterization Stability Assay of Beta-Galactosidase Storage time (days) The enzyme stored in 4 o C

scFv selection method Specificity test: Flow cytometry C11 48 scFvs 14 scFvs and A2 Randomly chosen Negative ELISA control test Antibody 111 11 scFvs 8 scFvs Library scFvs Biotinylated Ag Top 8 clones 85 + scFv Source : Ferrara, F., et al. (2012)

scFv Selection method Source : Ferrara, F., et al. (2012)

Advantages of our biosensor

Safer System

Easily distributed

Production Cost

Lab Capacity 22.73% Weak / limited Sufficient 77.27 % Source: http://whqlibdoc.who.int/publicatio ns/2011/9789241501330_eng.pdf

How big is the impact a better diagnostic tool will give?

Future Direction 9 6 million million Source: http://www.stoptb.org/assets/documents/ne ws/Reducing%20TB%20deaths%20among%20 HIV-positives%2023nov11.pdf

Human Practice • Synthetic bio club • Sharing with Paris Bettencourt team:  TB is a more complicated problem for woman  Developing countries is not ready yet to apply synthetic biology  Creation of safer system to apply synthetic biology in developing countries is needed

Parts Submitted • BBa_K1182425 Split β -Galactosidase α Fragment [improvement of BBa_K823016] • BBa_K1182000 Split β -Galactosidase ω Fragment [improvement of BBa_K891003] • BBa_K1182007 Antigen85- Biosensor α Fragment • BBa_K1182008 Antigen85- Biosensor ω Fragment • BBa_K1182010 (G4S)4 Linker • BBa_K1182011 Loxp511 Linker • BBa_K1182401 Forward Primer β -Galactosidase ω Fragment • BBa_K1182501 Reverse Primer β -Galactosidase ω Fragment

Sponsors