• aRGEN (Synthesis, Validation) CRISPR/CAS9 • dRGEN (Synthesis, Validation) • CAS9 protein & mRNA(WT, Nickase, Dead, Cy3) products • Surrogate reporter • Human-AAVS1 / Mouse-Rosa26 Kit. • KO / KI cell line Genome • KO / KI / cKO Mouse Editing service • KO Rat • Donor synthesis (ssDNA, dsDNA) In-del Analysis • Genotyping service • Only Miseq. running
Gene Function study Mutagenesis study Ex vivo, In vivo, Cell therapy, Gene therapy Animal model, Cell line model Stem cell engineering, plant & Animal genome editing (Molecular Breeding) Micro algae engineering, fungi, insect 1
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Species End product Gene Type • Human • One • Living • KO organism • Double KO • Mouse • Multi • KI • Cell line • Double KI • Rat • cKO • Primary cell (reversion) • Zebrafish (base editing) Confirm Transfect Detect gRNA+Cas9 • Transfection • In-del • Reagent • RNA + Protein analysis Marker (RNP) (NGS) • Electroporation • DNA + DNA • Surrogate (one, two vector) • Genotyping reporter • Lentivirus, AAV • DNA + Protein • RNA + mRNA 3
* Activity 별도 확인 필요 Cat No# TGEN_aRS1 – Product : aRGEN(sgRNA. 50ug) +pRGEN-Cas9-CMV/T7 (5ug) – Service Period : 2~3 week Target gene gRNA Design by ToolGen – Warranty period : 4 month gRNA form : RNA ( 액체 or 동결 건조 형태로 제공 ) CAS9-CMV plasmid 제공 (Protein 형태는 별도 구매 ) * off-target information 4
* Activity 별도 확인 필요 Cat No# TGEN_aRS1 – Product : aRGEN(sgRNA. 50ug) +pRGEN-Cas9-CMV/T7 (5ug) – Service Period : 2~3 week – Warranty period : 4 month Target gene gRNA Design by ToolGen gRNA form : RNA ( 액체 or 동결 건조 형태로 제공 ) CAS9-CMV plasmid 제공 (Protein 형태는 별도 구매 ) 5
* Activity 확인 완료 Cat No# TGEN_aRV1 gDNA(1~2ug) be given for other than Human / Mouse – Product : aRGEN(sgRNA. 50ug) + pRGEN-Cas9-CMV/T7 (5ug) + Purified Cas9 protein (50ug) → In vitro assay Target gene gRNA Design by ToolGen – Service Period : 4~5 week gRNA form : RNA ( 액체 or 동결 건조 형태로 제공 ) In vitro assay 로 Activity 확인된 gRNA 제공 ( 모든 종 에서 가능 ) – Warranty period : 4 month CAS9-CMV plasmid & CAS9 protein 모두 제공 Cat No# TGEN_aRV2 Cell be given for other than Human / Mouse – Product : aRGEN(sgRNA. 50ug) + pRGEN-Cas9-CMV/T7 (5ug) + Purified Cas9 protein (50ug) → Cell line assay Target gene gRNA Design by ToolGen – Service Period : 4~5 week gRNA form : RNA ( 액체 or 동결 건조 형태로 제공 ) – Warranty period : 4 month In cell(in vivo) assay 로 Activity 확인된 gRNA 제공 ( Only Human & Mouse ) CAS9-CMV plasmid & CAS9 protein 모두 제공 HEK293, NIH3T3 6
* Activity 확인 완료 Cat No# TGEN_aRSP1 – Product : Postive control_EGFP sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_aRSP2 – Product : Postive control_HPRT sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_aRSP3 – Product : Postive control_CCR5 sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month Target gene gRNA Design by ToolGen gRNA form : RNA ( 액체 or 동결 건조 형태로 제공 ) CAS9-CMV plasmid 제공 (Protein 형태는 별도 구매 ) * gRNA(aRGEN) 구매 시에 activity Positive control 로 사용할 수 있는 제품 7
* Activity 별도 확인 필요 Cat No# TGEN_dRS1 Promoter – Product : 1 dRGEN ( sgRNA plasmid, 1~2ug) + pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week Target gene gRNA Design by ToolGen – Warranty period : 4 month gRNA form : DNA ( 액체 형태로 제공 ) CAS9-CMV plasmid 제공 Promoter Cat No# TGEN_dRS2 – Product : 1 dRGEN ( sgRNA plasmid, 50ug) + pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week Target gene gRNA Design by ToolGen – Warranty period : 4 month gRNA form : DNA ( 액체 형태로 제공 ) 고농도 로 제공 (Midi prep. 으로 제공 ) CAS9-CMV plasmid 제공 Cat No# TGEN_dRPS1 – Product Target gene gRNA Design by ToolGen • 3 dRGEN ( sgRNA plasmid, 1~2ug) gRNA form : DNA ( 액체 형태로 제공 ) • pRGEN-Cas9-CMV/T7(5ug) target 에 대한 gRNA 3 개 제공 – Service Period : 2~3 week CAS9-CMV plasmid 제공 – Warranty period : 2 month Cat No# TGEN_dRPS2 – Product Target gene gRNA Design by ToolGen • 3 dRGEN ( sgRNA plasmid, 50ug) gRNA form : DNA ( 액체 형태로 제공 ) • pRGEN-Cas9-CMV/T7(5ug) target 에 대한 gRNA 3 개 제공 – Service Period : 2~3 week 고농도 로 제공 (Midi prep. 으로 제공 ) – Warranty period : 2 month CAS9-CMV plasmid 제공 8
* Activity 별도 확인 필요 Cat No# TGEN_dRS3 – Product : CRISPR-GFP plasmid(dRGEN, 1~2ug)+ pRGEN-Cas9-CMV/T7(5ug) Target gene gRNA Design by ToolGen – Service Period : 2~3 week gRNA form : DNA ( 액체 형태로 제공 ) – Warranty period : 4 month GFP 형광으로 Transfection efficiency check ( 세포내에서 계속적으로 발현하지는 않음 ) 저 / 고농도 로 제공 Cat No# TGEN_dRS4 CAS9-CMV plasmid 제공 – Product : CRISPR-GFP plasmid(dRGEN, 50ug)+ pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_OP1 – Product : able to buy individual pRGEN-Cas9-selection marker – pRGEN-Cas9-CMV/T7 – pRGEN-Cas9-CMV/T7 (wild type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-Ef1a (wild type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-CMV/T7 (nickase(D10A) type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-Ef1a (nickase(D10A) type or Puro-RFP or Hygro-EGFP) – Service Period : 2 week – Warranty period : 4 month 9
* Activity 확인 완료 Cat No# TGEN_dRV1 Possible to provide only in the Human/Mouse – Product • Validated 1 dRGEN ( sgRNA plasmid, 1~2ug) • pRGEN-Cas9-CMV/T7(5ug) • data/setup condition (T7E1 assay) Target gene gRNA Design by ToolGen → possible to provide additional dRGEN 1ea gRNA form : DNA ( 액체 형태로 제공 ) Transfection 진행 후 T7e1 assay 로 Activity 확인된 gRNA 제공 – Service Period : 4~5 week ( only Human & Mouse ) – Warranty period : 4 month CAS9-CMV plasmid 제공 Cat No# TGEN_dRV2 Possible to provide only in the Human/Mouse – Product • Validated 1 dRGEN ( sgRNA plasmid, 1~2ug) • pRGEN-Cas9-CMV/T7(5ug) • data/setup condition (FACS assay) → possible to provide additional dRGEN & Reporter 1ea – Service Period : 4~5 week Target gene gRNA Design by ToolGen – Warranty period : 4 month gRNA form : DNA ( 액체 형태로 제공 ) Human cell 에 Transfection 진행 후 FACS assay 로 Activity 확인된 gRNA 제공 ( 모든 종 확인 가능 ) Surrogate reporter 제공 (MACS/Hygro/RG2S) CAS9-CMV plasmid 제공 10
Cat No# TGEN_CP1 – Product : Cas9 WT protein, Injection ready, lyophilized,50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CP2 – Product : Cas9 WT protein, Injection ready, lyophilized, 100ug – Service Period : 2 week – Warranty period : 4 month form : Protein ( 액체 or 동결 건조 형태로 제공 ) 다양한 농도 및 양으로 제공 Cat No# TGEN_CP3 – Product : Cas9 WT protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CP4 – Product : Cas9 WT protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_Cy3P1/2 – Cy3 Cas9 protein, solution, 25μg/50ug form : Protein ( 액체 형태 로 제공 ) 다양한 농도 및 양으로 제공 가능 Cy3 형광 tagging 으로 check 가능 11
Cat No# TGEN_CNP1 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNP2 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 100ug – Service Period : 2 week form : Protein – Warranty period : 4 month ( 액체 or 동결 건조 형태로 제공 ) 다양한 농도 및 양으로 제공 가능 Cat No# TGEN_CNP3 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNP4 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month 12
Cat No# TGEN_CDP1 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CDP2 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 100ug – Service Period : 2 week form : Protein – Warranty period : 4 month ( 액체 or 동결 건조 형태로 제공 ) 다양한 농도 및 양으로 제공 가능 Cat No# TGEN_CDP3 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CDP4 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_Cy3DP2/4 – Cy3 Cas9 dead protein, solution, 50ug/250ug form : Protein ( 액체 형태 로 제공 ) 다양한 농도 및 양으로 제공 가능 Cy3 형광 taq 으로 check 가능 13
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