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Principles of Microscopy II: Super-resolution Humberto Cabrera - PowerPoint PPT Presentation

Jun 04, 2023 •9 likes •229 views

Principles of Microscopy II: Super-resolution Humberto Cabrera Venezuelan Institute for Scientific Research International Centre for Theoretical Physics Preparatory School to the Winter College on Optics: Advanced Optical Techniques for Bio-

  1. Principles of Microscopy II: Super-resolution Humberto Cabrera Venezuelan Institute for Scientific Research International Centre for Theoretical Physics Preparatory School to the Winter College on Optics: Advanced Optical Techniques for Bio- imaging

  2. Diffraction limit of imaging techniques

  3. The diffraction limit

  4. The diffraction limit of light

  5. The diffraction limit Molecules within the región < 200 nm are not discernible

  6. STED microscopy

  7. Experimental setup

  8. Confocal versus STED microscopy

  9. Applications of STED microscopy

  10. STED, RESOLFT microscopy

  11. STED, RESOLFT microscopy

  12. Total internal reflection fluorescence (TIRF) microscopy

  13. Principles of TIRF microscopy

  14. Experimental setup

  15. Experimental setup

  16. Experimental setup

  17. Experimental setup

  18. Experimental setup

  19. Experimental setup

  20. Applications

  22. Thanks

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