Pla nt Re se a rc h Workshop Gre e nhouse Re se a rc h a nd L a - PowerPoint PPT Presentation

Pla nt Re se a rc h Workshop Gre e nhouse Re se a rc h a nd L a bora tory Sa fe ty Offic e of Biologic al Safe ty Ca rrie Smith, Ph.D. Risk Ma na g e me nt Spe c ia list, Sr .

Goals of Biosafe ty/ Biose c ur ity  Pr ote c t pe r sonne l  Pre ve nt pe rso nne l fro m ha za rdo us ma te ria l  Pr ote c t the e nvir onme nt  Co nta in re g ula te d ma te ria l inside the g re e nho use  Pr ote c t the inte gr ity of the e xpe r ime nt  Pre ve nt unwa nte d pe sts a nd c o nta mina nts fro m e nte ring the g re e nho use  Co nta in e xpe rime nta l ma te ria l to pre ve nt c ro ss c o nta mina tio n o f e xpe rime nts

Re g ula te d Ma te ria ls T r ansge nic or ganisms (GMOs)  Pla nts  Mic ro b e s  F ung i  Ba c te ria  Viruse s  Anima ls  Arthro po ds  Ne ma to de s

Re g ula te d Ma te ria ls Othe r Mate r ials  No n-Wisc o nsin iso la te s o f pa tho g e ns  E xo tic spe c ie s  No xio us we e ds  Huma n pa tho g e ns a nd o ppo rtunistic pa tho g e ns

Risk Asse ssme nt  Orig in  Sta b ility in e nviro nme nt o utside o f g re e nho use  T ype a nd se ve rity o f dise a se (pa tho g e ns)  T ra nsmissib ility  Ca pa b ility to c o ntro l if a re le a se o c c urs  Me a ns fo r c o nta inme nt  Physic a l c o nta inme nt  Bio lo g ic a l c o nta inme nt  Pro c e dure s a nd pra c tic e s

Ove rsig ht  Re g ula tory Ag e nc ie s  Inte rna l Ove rsig ht  I nstitutio na l Bio sa fe ty Co mmitte e (I BC)  Offic e o f Bio lo g ic a l Sa fe ty (OBS)  Inte rna l Polic ie s  UW I nstitutio na l Bio sa fe ty Co mmitte e Ha ndb o o k  Gre e nho use fa c ility po lic ie s

Re g ula to ry a nd Guida nc e Do c ume nts  NI H Guide line s  BMBL  Gre e nho use c o nta inme nt g uide  Arthro po d c o nta inme nt g uide

T he Impor tanc e of Biosafe ty/ Biose c ur ity  3 Go a ls o f Bio sa fe ty/ Bio se c urity  Pro te c t the e nviro nme nt  Pro te c t pe rso nne l  Pro te c t inte g rity o f the re se a rc h  No nc o mplia nc e c o nse q ue nc e s  Suspe nsio n o r te rmina tio n o f re se a rc h a c tivitie s a nd funding  Re se a rc he r in vio la tio n  Othe r re se a rc he rs a t UW  F ine s (e .g . up to $250,000 pe r vio la tio n fo r no t a dhe ring to pe rmit re q uire me nts)  Unive rsity  Re se a rc he r  Da ma g e d pub lic pe rc e ptio n o f UW

Gre e nho use Bio sa fe ty/ Bio se c urity is a Re se a rc h Pe rso nne l Gre e nho use Pe rso nne l I nstitutio na l Bio sa fe ty Offic e o f Bio lo g ic a l Co mmitte e Sa fe ty

What mate r ials ne e d to be c ontaine d? I f the pla nt is tra nsg e nic ? I f the pla nt is ino c ula te d with a mic ro b e ?

How c an r e gulate d mate r ial ge t out of the gr e e nhouse ? Inse c ts Wind/ fans dispe r sal Stic k to c lothing Down the dr ain Bir ds Stic k to shoe s T hr own out be for e Contaminate d inac tivation E quipme nt

Containme nt Str ate gie s  Physic a l Co nta inme nt  Bio lo g ic a l Co nta inme nt  Pra c tic e s a nd Pro c e dure s

Physic al Containme nt Co nta inme nt c ha mb e r F a c ility struc ture

Physic a l Co nta inme nt Wa te r c a pture de vic e Ve stib ule

Physic a l Co nta inme nt I nse c t do rm Pla nt tra ys

Physic a l Co nta inme nt Dra in so c k Ba g re pro duc tive struc ture s

Physic a l Co nta inme nt Do o r swe e p Stic ky ma t

Biologic al Containme nt  Stra te g ie s to ke e p tra nsg e ne s o ut o f po lle n/ se e d  Use ste rile line s  Use Vira l I nduc e d Ge ne Sile nc ing (VI GS) to intro duc e tra nsg e ne s  I nse rt tra nsg e ne s into the c hlo ro pla st Ste rile ma le F e rtile ma le

Biologic al Containme nt  Muta nt mic ro b e s  Virus la c king g e ne (s) re q uire d fo r tra nsmissio n via inse c t  Mic ro b e s with re duc e d ho st ra ng e  Mic ro b e s tha t c a nno t pro duc e surviva l struc ture s

Biologic al Containme nt  Gro w pla nts in a lo c a tio n whe re if the re wa s a re le a se the pla nts wo uld no t survive  Gro w pla nts during a se a so n in whic h if the re wa s a re le a se the pla nts wo uld no t survive  Gro w pla nts during a time whe n ne a rb y se xua lly c o mpa tib le pla nts a re no t re c e ptive

Ho w a re the se pla nts diffe re nt ? I no c ula te d T ra nsg e nic WT with a virus

Communic ation and Signage  T ra nsg e nic vs. no ntra nsg e nic  I no c ula te d vs. unino c ula te d (inc lude da te )  Pro je c t spe c ific re q uire me nts  Sig na g e o n g re e nho use b a y do o r  L a b e ls o n pla nts/ tra ys o f pla nts  Co nta c t info rma tio n

Ke e ping T hings T idy and Cle an

Contain Mate r ial Dur ing T r anspor t

Disposal/ Disinfe c tion Pr oc e dur e s T ra nsg e nic ma te ria l must b e “re nde re d b io lo g ic a lly ina c tive b e fo re dispo sa l” Pla nt ma te ria l, so il, po ts, a nd g ro wth a re a

E me r ge nc ie s/ Ac c ide nts  Re le a se o f tra nsg e nic ma te ria l o utside o f fa c ility  Spills o utside o f c o nta inme nt  E xpo sure to b io ha za rdo us ma te ria l  F irst a id a nd me dic a l a tte ntio n ta ke s prio rity o ve r re po rting  F irst re po rt is in a dditio n to the E mplo ye e ’ s Wo rk I njury a nd I llne ss Re po rt

Summar y  3 Go a ls o f Bio sa fe ty/ Bio se c urity  Pro te c t pe rso nne l  Pro te c t the e nviro nme nt  Pro te c t the inte g rity o f the e xpe rime nt  Co nta inme nt Stra te g ie s  Physic a l Co nta inme nt  Bio lo g ic a l Co nta inme nt  Pra c tic e s a nd Pro c e dure s

T hings to Re me mbe r  K no w the b io lo g y o f the o rg a nisms yo u a re wo rking with  Be a wa re o f the c o nditio ns se t fo rth b y re g ula to ry a g e nc ie s a nd the I nstitutio na l Bio sa fe ty Co mmitte e fo r yo ur pa rtic ula r pro je c t  Utilize g re e nho use pe rso nne l a nd the o ffic e o f b io lo g ic a l sa fe ty, we a re he re to he lp!

Contac t Info Ca rrie Smith, Ph.D. Risk Ma na g e me nt Spe c ia list, Sr. Offic e o f Bio lo g ic a l Sa fe ty E nviro nme nt, He a lth & Sa fe ty 890-4951 c smith@ fpm.wisc .e du www.e hs.wisc .e du