ODR-10 expressed in human HEK293 cells Ado pte d fro m Y. Zha ng , - PowerPoint PPT Presentation

- A Synthe tic Pa thwa y fo r Pe st Co ntro l

ODR-10 expressed in human HEK293 cells Ado pte d fro m Y. Zha ng , e t a l, 1997.

Oute r Me mbr ane Inne r GDP G α GT P G α G β G γ G β MAPK G γ pa thwa y F US1 F AR1 Ce ll Ma ting c yc le a rre st

N Rat RI7 odor ant r e c e ptor C Oute r Ce llular 1 2 3 4 5 6 7 N C ODR- 10 r e c e ptor me mbr ane Inne r

Chime r ic 1 2 3 4 5 6 7 r e c e ptor GDP GT P Ga olf Gpa1 G α olf Gpa1 G β G γ G β G γ MAPK pa thwa y F AR1 F US1 Ce ll c yc le a rre st

T =45min T =90min (a) (b) (c) GAL 1 Chime r ic r e c e ptor GF P te r minator Induc e d by Galac tose Not induc e d

Chime r ic r e c e ptor RI7 r e c e ptor (1) (3) 4h 4h L igand 2h 2h induc tion 0h 0h 1C 2C 1C 2C Diac e tyl He xanal GAL 1 Chime r ic r e c e ptor te r minator GAL 1 Rat RI7 r e c e ptor te r minator (4) (2) 4h 4h Contr ol 2h 2h 0h 0h 1C 2C 1C 2C No Diac e tyl No He xanal

α α fa c tor fa c tor 25 μ m

Oute r Me mbr ane Inne r GDP Gα Gα olf GT P Gα olf Gα G β G γ G β MAPK G γ pa thwa y GPA1 F AR1 F US1 F AR1 Ce ll Ma ting c yc le a rre st

2-phenylethanol • Attra c ts dro so phila • Vo la tile • Pro duc e d in ye a st e ndo g e no us pa thwa y

ARO9 Ar o9p YDR380W YDL 168W 2- phe nylalanine phe nylpyr uvate phe nylac e talde hyde 2- phe nyle thanol

α factor Odorant ARO9 α factor ARO9 Odorant MAPK MAPK GF P 2-phenylethanol

GAL1 GAL10 BinA Terminator Terminator BinB GST GST

 Co nstruc tio n o f the RI 7-ODR-10 c hime ric re c e pto r  L o c a liza tio n a nd func tio na l a ssa y o f the c hime ric re c e pto r in S. c e re visiae  Co nstruc tio n o f a Δ GPA1 , Δ F AR1, Δ ARO9 S. c e re visiae stra in  Co ntrib utio n o f Bio Bric ks to the Re g istry  Cha ra c te riza tio n o f Bio Bric k Pa rts (BBa _K 211001;BBa _K 211002)

 Optimiza tio n o f the GPCR c o uple d pa thwa y fo r mo re e ffic ie nt o do ra nt se nsing  Ong o ing c o nstruc tio n o f the a ttra c ta nt pro duc ing c irc uit a nd c ha ra c te riza tio n o f the re po rte r func tio na l a ssa y  Ong o ing c o nstruc tio n o f the to xin pro duc ing c irc uit a nd b io lo g ic a l te st

RI7N L igand binding domain RI7C MAPK Attractant F US1 pr omote r F US1 te r minator

Curbing the population of pathogen vectors

Model system to screen for GPCRs that bind to certain ligands. ODR- 10 ligand binding RI7N RI7C domain MAPK MAPK GF P hormones, odorants, neurotransmitters, medicines…

Instr uc tor and Advisor s fr om HKUST : • Pro fe sso r K ing L . CHOW • Gua ng na n T I AN • Mr. Wa i-Pa ng HO Othe r s: • Pro fe sso r Chun L I ANG a nd Mr. Re ntia n WU, HK UST • Pro fe sso r Da vid K a rl BANF I E L D, HK UST • Dr. Je ssic a Ce Mun T ANG, HK UST • Pro fe sso r Stua rt F I RE ST E I N, Co lumb ia Unive rsity • Pro fe sso r Pia li SE NGUPT A, Bra nde is Unive rsity • Dr. Bo o nhia ng PROMDONK OY, BI OT E C • Dr. E dith PAJOT -AUGY, I NRA- Unive rsité Pa ris-Sud • L e e -Hysa n F o unda tio n

- A Synthe tic Pa thwa y fo r Pe st Co ntro l