Nerolidol-mediated rendezvous of dynastid beetles in long melon - PowerPoint PPT Presentation

2018 ESA,ESC and ESBC joint Annual Meeting Nerolidol-mediated rendezvous of dynastid beetles in long melon flowers Arodi P. Favaris; Amanda C. Túler; Weliton D. Silva; Walter S. Leal; José Maurício S. Bento Arodí Prado Favaris

Lagenaria siceraria (Curcubitaceae)

Lagenaria siceraria (Curcubitaceae) Long melon/Bottle gourd plant

Cyclocephala paraguayensis (Coleoptera, Melolonthidae, Dynastinae)

The genus Cyclocephala (tribe Cyclocephalini) Beetle flower association flower volatiles attraction mating food aggregation shelter opportunity resource Gottsberger, Pl.Syst.Evol. (1986)

The genus Cyclocephala (tribe Cyclocephalini) Beetle flower association flower volatiles attraction mating food aggregation shelter opportunity resource Gottsberger, Pl.Syst.Evol. (1986)

The genus Cyclocephala (tribe Cyclocephalini) Beetle flower association flower volatiles attraction mating food aggregation shelter opportunity resource Gottsberger, Pl.Syst.Evol. (1986)

Do long melon flower volatiles attract Cyclocephala paraguayensis ?

Methods Flower volatile collection battery vaccum pump / adsorbent polymer GC-EAD Chemical identification GC-MS/ synthetic standard Field trial Filter paper ‘ flowers ’ Treated: 100 μ L synthetic compound Control: no compound

Methods Flower volatile collection battery vaccum pump / adsorbent polymer GC-EAD Chemical identification GC-MS/ synthetic standard Field trial Filter paper ‘ flowers ’ Treated: 100 μ L synthetic compound Control: no compound

Methods Flower volatile collection battery vaccum pump / adsorbent polymer GC-EAD Chemical identification GC-MS/ synthetic standard Field trial Filter paper ‘ flowers ’ Treated: 100 μ L synthetic compound Control: no compound

Methods Flower volatile collection battery vaccum pump / adsorbent polymer GC-EAD Chemical identification GC-MS/ synthetic standard Field trial Filter paper ‘ flowers ’ Treated: 100 μ L synthetic compound Control: no compound

Results GC-EAD aeration extract ♂ ♀

Results GC-EAD aeration extract ♂ ♀ Chemical identification: GC-MS % 69 100 93 75 41 50 107 43 67 119 25 136 161 148 189 204 175 222 241 252 271 279 0 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280

Results GC-EAD aeration extract ♂ ♀ Chemical identification: GC-MS % 69 100 93 75 41 50 107 43 67 119 25 136 161 148 189 204 175 222 241 252 271 279 0 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Trans-nerolidol

Results GC-EAD Field trial aeration extract ♂ ♀ Chemical identification: GC-MS % 69 100 93 75 41 50 107 43 67 119 25 136 161 Insect total catches: 148 189 204 175 222 241 252 271 279 0 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Treated: 6 Control: 0 Exact binomial test of goodness- of-fit ( P =0.03) Trans-nerolidol

Field experiment (Cassilândia, Brazil)

Field experiment (Cassilândia, Brazil) 300 280 250 Total number of insects/treatment 200 150 100 50 0 0 NEROLIDOL CONTROL Exact binomial test of goodness-of-fit ( P <0.01)

Conclusion Nerolidol contributes to Cyclocephala paraguayensis (Coleoptera: Melolonthidae) aggregation in long melon flowers, Lagenaria siceraria (Curcubitaceae)

Acknowledgments Contact: arodi@usp.br