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Multiplex arrays for Genotyping Multiplex arrays for Genotyping 2 - PowerPoint PPT Presentation

Jul 18, 2023 •138 likes •780 views

Multiplex arrays for Genotyping Multiplex arrays for Genotyping 2 Alere Technologies, Jena 3 4 Complex information Complex information 5 Jena & Zeiss 6 Arrays and reader systems Clondiag: Clondiag: 7 S. Aureus product development:

  1. Multiplex arrays for Genotyping Multiplex arrays for Genotyping

  2. 2 Alere Technologies, Jena

  3. 3

  4. 4 Complex information Complex information

  5. 5 Jena & Zeiss

  6. 6 Arrays and reader systems Clondiag: Clondiag:

  7. 7 S. Aureus product development:

  8. VLA Products • Detects ~57 antimicrobial resistance genes in Gram • Detects ~57 antimicrobial resistance genes in Gram negative bacteria • Detects ~92 virulence genes in E. coli • Detects ~95 virulence factors and toxin genes • Detects ~100 virulence factors and antimicrobial resistance genes in Gram positive bacteria resistance genes in Gram positive bacteria

  9. P bli Publications i • AMR-ve ArrayTube AMR A T b Development of a miniaturised micro-array for the rapid identification of antimicrobial resistance genes in Gram-negative g g bacteria Miranda Batchelor, Katie L Hopkins, Ernesto Liebana, Peter Slickers, Ralf Ehricht Muriel Mafura Frank Aarestrup Dik Mevius Felicity Clifton-Hadley Ehricht, Muriel Mafura, Frank Aarestrup, Dik Mevius, Felicity Clifton Hadley, Martin J Woodward, Rob H Davies, E John Threfall, Muna F Anjum; International Journal of Antimicrobial Agents 2008 May 31(5) p 440-451 • Ec ArrayTube P th t Pathotyping Escherichia coli by using miniaturised DNA microarrays i E h i hi li b i i i t i d DNA i Muna F Anjum, Muriel Mafura, Peter Slickers, Karin Ballmer, Peter Kuhnert, Martin J Woodward, Ralf Ehricht; Applied and Environmental Microbiology, pp gy 2007 Sept 73(17), p.5692-7

  10. Packs contain: Packs contain: • 25 ArrayTubes • 25 ArrayTubes • 5 foil packets containing 5 tubes in each • Each tube has unique number (AT Lot) E h t b h i b (AT L t) • Primer mix Primer mix • Freeze-dried • Reconstituted in 120µl water R tit t d i 120 l t • IFU and gene list IFU d li t • Labelling and hybridisation protocol • Buffers and reagent ordering information

  11. Buffers and reagents • Not supplied • Ordering information provided in IFU Ordering information provided in IFU

  12. Buffers and reagents IFU • Recipes outlined in IFU d i tli i R

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