Medic ical C l Center er f for Infectio ious s Disea seases - PowerPoint PPT Presentation

Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin

RPV HIV-GRADE update Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Resistant Imtermediate resistance Limited susceptibility • Score of 3 and • Score of 2 • Score of 1 higher 3 Points: 101P , 138K, 181IV, 230LI, 138RAGQS + 184I, 100I + 103SN, 100I + 103R + 179D 2 Points: 138RAGQS, 101E, 181C, 188L, 189I 1 Point: 90I, 100I, 221Y , 230V, 179L, 227C

DTG HIV-GRADE update Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Resistent Intermediäre Resistenz Eingeschränkte Empfindlichkeit • Q148HKR + 2 out of • Q148HKR + 1 out of • L74IM • E92Q (G140ACS, L74I, (G140ACS, L74I, • T97A E138AKT) E138AKT) • 148RH + 140S • N155H + 1 out of ( • G118R • E138AKT E92Q, T97A, Y143R, • Y143R L74IM, E138AKT) • R263K • Q148HKR • G140ACS • N155H • V260I • S147G Dosage adaptions for DTG according to the current version of the label should be considered.

Miscellaneous Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin • Renaming: • TDF/TAF instead of TDF • geno2pheno: • Update to new geno2pheno version • Connection by REST service • Automated update of the cut-offs • Interface add on • Output of result as JSON or XML

Work in progess Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin • Easier batch interpretation • Output as XLSX-spreadsheet • Update of interface • Configurable report • User specific defaults • Speed up of parser

Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin HIV2EU Ruleset

Nucleoside reverse transcriptase Inhibitors Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Substance Resistant Intermediate Resistance •Q151M •S215ACFLY AZT •S215ACFLY + 1 of (N69ST, K70R, Y115F, K223R) •Q151M •S215ACFLY d4T •S215ACFLY + 1 of (K65R, N69ST, •K65R K70R, Y115F, K223R) • not recommended* •M184VI •K65R 3TC/FTC • Q151M •2 of (M184VI, S215ACFLY, D67N, K70RN) ABC • K65R • M184VI+1 of (L74V , Y115F ) • K65R TDF/TAF • Q151M+V111I • K65R ddI • L74V * Not recommended due to rapid • Q151M resistance development •not recommended*

Non-nucleoside reverse transcriptase Inhibitors Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Not effective against HIV-2, not recommended due to natural resistance

Protease Inhibitors Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Substance Resistant Intermediate Resistance •G48V • I84V SQV •L90M •2 of (I82F, I84V, L90M) • V62A+ L99F LPV •I54M • 1 of (I82F, I84V, L90M) •V47A • I50V • 1 of (I84V, L90M) DRV • I54M • I84V + L90M • I50L • I54L ATV • I82F • ATV not recommended • natural resistance TPV •I54M IDV •I82F •I84V •V62A + L99F •L90M •IDV not recommended • natural resistance fAPV

Integrase Inhibitors Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Substance Resistant Intermediate Resistance • N155HR •E92Q RAL • Q148KR •Y143CGR • 1 of (E92Q, T97A) + Y143CGR • E92Q + T97A • E92QG • Y143C EVG* •Q148KR •T97A + Y143C •N155H •G140S + Q148R •Q148R DTG* •Q148K •N155H •E92Q + N155H •E92Q •T97A + N155H •Y143C + T97A *Rules based almost exclusively on in-vitro data

Fusion Inhibitors Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin T-20 not recommended

Tropism Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin For tropism determination use: Geno2pheno[coreceptor-HIV2]

Tropism Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Visseaux, Benoit, Margarita Hurtado-Nedelec, Charlotte Charpentier, Gilles Collin, Alexandre Storto, Sophie Matheron, Lucile Larrouy, Florence Damond, Françoise Brun-Vézinet, und Diane Descamps. „Molecular Determinants of HIV-2 R5–X4 Tropism in the V3 Loop: Development of a New Genotypic Tool“. Journal of Infectious Diseases 205, Nr. 1 (1. Januar 2012): 111–20. doi: 10.1093/ infdis/ jir698 .

External Quality Assessment Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin I NSTAND e.V.

EQA Results PR/RT Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin INSTAND e.V.

EQA results INT Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin

EQA results Tropism Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin

Medic ical C l Center er f for Infectio ious s Disea seases ses Berli lin Thank you Thomas Berg, Patrick Braun, Martin Däumer, Josef Eberle, Robert Ehret, Rolf Kaiser, Claudia Kücherer, Harm Müller, Christian Noah, Martin Obermeier, Martin Stürmer, Alexander Thielen, Jens Verheyen, Hauke Walter, Eva Wolf Special thanks to Marcel Schütze, Heinz Zeichhardt, Oliver Donoso and Hans-Peter Grunert