Mechanical Bioreactor Integrated Systems Amanda Castagnino Emily Adams Emily Wood Natalie Nold Simran Singh
Agenda
PHASE III GOALS PHASE III DELIVERABLES 1. Achieve specific device movement. 1. Machined device pieces 2. Machine parts for device mount. 2. Cell Culture Images 3. Complete static cell culture testing in 3. Updated Risk Assessment PDMS chamber. 4. Updated Bill of Materials 4. Complete material testing on heat-cured PDMS. 5. MSD II Project Plan
New Membrane Holder: Solving Sterility Issue
Update on Machining Timeline Submitted Parts 3/9 ● Miscommunication ● Miscommunication ● resolved 3/19 2 parts received by ● 3/27 Reached out to Mike, ● Debartolo, and Rick WIll have new time ● estimate 3/30
Machining Problem Tracking...
Identifying & Generating Selecting & Selecting Analyzing Potential Planning Implementing Evaluating Problem Problem Solutions Solution Solution Solution PSP 1 PSP 2 PSP 3 PSP 4 PSP 5 PSP 6 Rating Description R1 R2 R3 Y4 Y5 G6 Machining 5 hours put into Not enough trained Consult machine parts takes machining with no team members and Ask machine shop to shop and get time much longer parts completely experience to make machine parts for us estimate Major than expected finished designed parts open up 10 days since files unclear Connected with meeting with communication with Time submitted, only 2 parts communication with debartolo and machine shop shop and reach out done machine shop Houdini Friday Major to debartolo Incorrect Provided incorrect Lack of machining determine correct edit and resubmit files tapping tapping tolerances to knowledge size of tapped holes files resubmitted tolerances machine shop have not completed do no know how to delegate task to Sheet metal draw for membrane create sheet metal amanda, who will complete drawing drawing holder drawing learn how Pegs are not Pegged Plate Pegs Tapped poorly Confront shop Remake part perpendicular Complicated piece to needs welding and Houdini is Arm Consult Houdini machine press-fit making part Complicated piece to needs press-fit and Mold Consult Houdini Major machine high tolerances
Device Movement Update ● ● ●
Actuator Precision ● ● ● ●
% Strain Tolerances % Strain Stretch (mm) % Range w/ accuracy (using 1.4%) Varying thickness in 5 2 3.6 - 6.4 PDMS chamber could lead to a 10 4 8.6 - 11.4 greater proportion of stretching in the well 15 6 13.6 - 16.4 of the chamber. 20 8 18.6 - 21.4
Material Testing with Heat-Cured PDMS “N” Control PDMS cured at 100°C for 35 minutes. “I” Incubated at 37°C Testing tab slipping reduced with three days before heat-cured samples. material testing
Results
Results
Results Sylgard 184 TDS: Tensile Strength (6.7MPa) Strains: Standard (58.8%) - 43.9% Worst Case Incubator (55.5%) - 33.1% Worst Case
Static Cell Culture Images 4X Objective - Leica 10X Objective - Leica 2.5X Objective - Leica
Risk Assessment Limited project time increases risk
Bill of Materials Machining and cell culture supplies purchased Total Budget Left ~ $350
MSD II Project Plan - Final Phase Finish machining parts and creating cycling programs Test experiment conditions Complete user documentation Test with subjects as much as possible
Project Deadlines April 19 Finished final poster Technical paper submitted for review April 24 Finished EDGE website Performance vs Requirements Lightning Talk Slide April 26 Technical Paper due April 27 Industrial Advisory Board Demo April 28 Imagine RIT Festival May 2 Workspace clean May 2-4 Final Gate Review
Three-Week Plans Amanda Emily A. Emily W. Natalie Simran Dynamic cell Finalize technical Dynamic cell Finalize programs culture testing in report culture testing in for device PDMS chamber PDMS chamber Finish machining Create Imagine device parts Verify % strain Device 3-D RIT poster Create guide for movement and printing and cell imaging and tolerances machining Finalize device analysis documentation
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