Multifunctional Nanoparticles for Photothermally Controlled Drug Delivery and Magnetic Resonance Imaging Enhancement Kyung-Hwa Yoo (Yonsei University)
Multifunctional Nanoparticle Multifunctional Nanoparticle Photothermal therapy Au layer Photothermally Drug controlled drug delivery Biocompatible & biodegradable polymer Magnetic layer MRI contrast agents
Au nanoshell PLGA beads O 2 plasma PMMA PMMA Si substrate Si substrate PS beads deposition Au deposition Lift-off PMMA Si substrate
Visible/NIR spectra Visible/NIR spectra 1.0 (b) PS 30 nm Absorbance (a.u.) 20 nm 14 nm 15 nm 20 nm 25 nm 10 nm 0.5 400 600 800 1000 1200 400 600 800 1000 1200 Wavelength (nm) Wavelength (nm)
Hyperthermia 46 o C) PS-Au half-shell 44 Temperature ( 42 40 PS nanoparticle Au nanoparticle 38 Water 36 0 50 100 150 200 Time (s)
Hyperthermia (a) (b) C=145 µ g/ml 2 P=25 W/cm 45 45 2 P=25 W/cm 145 µ g/mL 40 40 2 P=15 W/cm 72.5 µ g/mL 35 35 36.25 µ g/mL 2 P=7 W/cm 30 30 0 50 100 150 200 0 50 100 150 200 Time (s) Time (s)
Photothermal toxicity Photothermal toxicity (laser 15a) (laser 15a) Calcein-AM Calcein-AM Bright field image Bright field image Without particle Without particle Laser Laser 15a 50 min 15a 50 min With particle With particle incubation incubation (100ug/ml) 1 day (100ug/ml) 1 day Laser Laser 15a 15 min 15a 15 min 50 min 50 min
Drug Release with & without NIR irradiation Drug Release with & without NIR irradiation (a) 100 -1 slope = 18.059 day 80 Cumulative % Released 60 -1 slope = 9.0738 day 40 with NIR irradiation 20 without NIR irradation 0 0 1 2 3 4 Time (day)
(b) With NIR illumination 0 min. 15 min. 50 min. 2 μ m 2 μ m 2 μ m (b) Without NIR illumination 0 min. 15 min. 50 min. 2 μ m 2 μ m 2 μ m
TIRFM images TIRFM images (a) 15 min. 0 min. 50 min. 10 min. 300 nm (b) 0 min. 10 min. 15 min. 50 min. 300 nm
0 day After 2-day NIR irradiation After 3 days without NIR irradiation After 3-day NIR irradiation
(b) (a) 60 0 day 1 day 2 day 60 Temperature ( ° C) Absorbance (a.u.) 3 day 0.40 55 50 7 day 50 o C ) Temperature ( 45 40 40 35 0.38 30 25 20 0 5 10 15 20 30 Time (min) 0.36 0 1 2 3 700 800 900 Wavelength (nm) Time (min.)
(a) (b) Au 0.36 Mn (c) Absorbance (a.u.) Mn(10 nm)/Au(25 nm) H-S NPs 0.32 Mn(0 nm)/Au(25 nm) H-S NPs 4 1/T 2 (1/sec.) Mn (10 nm)/Au (25 nm) 2 0.28 Mn (10 nm)/Au (0 nm) 0 0.0 0.5 1.0 1.5 400 600 800 1000 Wavelength (nm) Relative nanoparticle concentration
Acknowlegement Acknowlegement Huiyul Park; Sungbaek Seo, Kyujung Kim (Nanomedical Graduate Program) Yang, Jae Moon; Haam, Seung Ju (Dept. of Chemical Engineering) Dong hyun Kim (Depat. of Electrical and Electronic Engineering Jinsuk Suh (Dept. of Radiology) * Funding: KOSEF through National Core Research Center for Nanomedical Technology
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