iTRAQ-based quantitative proteomic analysis reveals important - PDF document

iTRAQ-based quantitative proteomic analysis reveals important metabolic pathways for arsenic-induced liver fibrosis in rats Shunhua Wu 1 *, Jing Li 1 , Xiang Jin 1,2 1. Department of Occupational and Environmental health, School of public health, Xinjiang Medical University, Urumqi, 830011, China 2. Shenzhen Omics Medical Research Center, Shenzhen, 518053, China Running title: proteomic analysis of rat liver under arsenite stress

Supplementary Information Supplementary Table S1. 431 differentially expressed proteins that is common in high dose group and medium dose group than in control group. Supplementary Table S2. KEGG analysis of 431 differentially expressed genes Supplementary Table S3. Gene ontology annotation of the 431 differentially expressed genes. Supplementary Figure S1. Tree-view of the GO enrichment analysis of biological process category for the 171 proteins that decreased in both medium and high dose treatment. Supplementary Figure S2. Tree-view of the GO enrichment analysis of biological process category for the 260 proteins that increased in both medium and high dose treatment. Supplementary Figure S3. Tree-view of the GO enrichment analysis of cellular component category for the 171 proteins that decreased in both medium and high dose treatment. Supplementary Figure S4. Tree-view of the GO enrichment analysis of cellular component category for the 260 proteins that increased in both medium and high dose treatment. Supplementary Figure S5. Tree-view of the GO enrichment analysis of molecular function category for the 260 proteins that increased in both medium and high dose treatment.

GO:0008150 biological_process GO:0071840 GO:0032501 GO:0050789 GO:0022610 GO:0032502 GO:0044699 GO:0051179 cellular component GO:0009987 GO:0008152 GO:0050896 GO:0065007 multicellular organismal regulation of biological biological adhesion developmental process single-organism process localization organization or cellular process metabolic process response to stimulus biological regulation process process biogenesis GO:0048856 GO:0044707 GO:0044767 GO:1902578 GO:0051234 GO:0033036 GO:0044763 GO:0016043 GO:0044085 GO:0044710 GO:0071704 GO:0006807 GO:0044237 GO:0051716 GO:0065008 GO:0050794 GO:0032879 GO:0048519 GO:0003008 GO:0007155 GO:0051641 GO:0006950 GO:0042221 anatomical structure single-multicellular single-organism single-organism establishment of macromolecule single-organism cellular cellular component cellular component single-organism metabolic organic substance nitrogen compound cellular metabolic cellular response to regulation of biological regulation of cellular regulation of negative regulation of system process cell adhesion cellular localization response to stress response to chemical development organism process developmental process localization localization localization process organization biogenesis process metabolic process metabolic process process stimulus quality process localization biological process GO:0034641 GO:0006725 GO:0050877 GO:0098602 GO:0009653 GO:0007275 GO:0051649 GO:0070727 GO:0006928 GO:0048869 GO:0030030 GO:0043062 GO:0043933 GO:0022607 GO:1901135 GO:1901360 GO:1901564 GO:0046483 GO:0033554 GO:0051128 GO:0098609 GO:0008104 GO:0006996 GO:0044238 cellular nitrogen cellular aromatic neurological system single organism cell anatomical structure multicellular organism establishment of cellular macromolecule movement of cell or cellular developmental cell projection extracellular structure macromolecular complex cellular component carbohydrate derivative organic cyclic compound organonitrogen compound heterocycle metabolic cellular response to regulation of cellular cell-cell adhesion protein localization organelle organization primary metabolic process compound metabolic compound metabolic process adhesion morphogenesis development localization in cell localization subcellular component process organization organization subunit organization assembly metabolic process metabolic process metabolic process process stress component organization process process GO:0006139 GO:0072521 GO:0016337 GO:0032989 GO:0034613 GO:0030029 GO:1902589 GO:0030198 GO:0071822 GO:0065003 GO:0070271 GO:1901657 GO:0044281 GO:0006793 GO:0031344 GO:0007600 GO:0048731 GO:0030154 GO:0007010 nucleobase-containing purine-containing single organismal cellular component cellular protein actin filament-based single-organism organelle extracellular matrix protein complex subunit macromolecular complex protein complex glycosyl compound small molecule metabolic phosphorus metabolic regulation of cell sensory perception system development cell differentiation cytoskeleton organization compound metabolic compound metabolic cell-cell adhesion morphogenesis localization process organization organization organization assembly biogenesis metabolic process process process projection organization process process GO:0055086 GO:0006796 GO:0050953 GO:0034109 GO:0007399 GO:0030036 GO:0019637 GO:0048513 GO:0000902 GO:0048468 GO:0006461 nucleobase-containing phosphate-containing sensory perception of homotypic cell-cell nervous system actin cytoskeleton organophosphate metabolic animal organ development cell morphogenesis cell development protein complex assembly small molecule metabolic compound metabolic light stimulus adhesion development organization process process process GO:0007015 GO:0009116 GO:0006753 GO:0019693 GO:0007601 GO:0007423 GO:0022008 GO:0051259 actin filament nucleoside metabolic nucleoside phosphate ribose phosphate visual perception sensory organ development neurogenesis protein oligomerization organization process metabolic process metabolic process GO:0051291 GO:0009119 GO:0042278 GO:0009123 GO:0009117 GO:0009132 GO:0001654 GO:0048699 GO:0016310 protein ribonucleoside metabolic purine nucleoside nucleoside monophosphate nucleotide metabolic nucleoside diphosphate eye development generation of neurons phosphorylation heterooligomerization process metabolic process metabolic process process metabolic process GO:0009126 GO:0009161 GO:0009135 GO:0009185 GO:0043010 GO:0046128 GO:0006163 GO:0046939 GO:0009259 GO:0030182 purine nucleoside ribonucleoside purine nucleoside ribonucleoside camera-type eye purine ribonucleoside purine nucleotide nucleotide ribonucleotide metabolic neuron differentiation monophosphate metabolic monophosphate metabolic diphosphate metabolic diphosphate metabolic development metabolic process metabolic process phosphorylation process process process process process GO:0009167 GO:0009179 GO:0060041 GO:0009150 GO:0006165 purine ribonucleoside purine ribonucleoside retina development in purine ribonucleotide nucleoside diphosphate monophosphate metabolic diphosphate metabolic camera-type eye metabolic process phosphorylation process process GO:0046031 ADP metabolic process