Isobionics Company profile Founded in 2008 Our products Flavour - PowerPoint PPT Presentation

Isobionics ‐ Company profile  Founded in 2008  Our products – Flavour & Fragrance isoprenoids: Valencene / Nootkatone / Beta‐Bisabolene Valencene  F&F product pipeline: Citrus terpenoids, sandalwood, patchouli, C10 – C20 terpenes  R&D laboratories for strain engineering, fermentation, downstream processing, and analytics Nootkatone  Strong scale‐up and production capabilities With Valencene, Isobionics was the first company bringing a Beta-Bisabolene fermentation‐based terpene flavour to the market Proprietary Material Do not Copy

Our products: isoprenoids Built from isoprene (C5) units Largest group of natural hydrocarbons >50.000 identified compounds Low natural abundance Isobionics technology: scalable, cost‐effective production via fermentation Isoprenoid ingredients markets:  Food: Vitamins (A,E), Sweeteners (Steviol)  Pharmaceuticals (Taxol)  Chemical building blocks (Isoprene)  Flavors & Fragrances Strictly Confidential – subject to confidentiality provisions Distribution and copying not allowed

F&F isoprenoids We have developed the technology to allow production of isoprenoids via fermentation  Monoterpenes (C10)  Sesquiterpenes (C15) C10 – Monoterpenes  Diterpenes (C20) Isoprenoids occur naturally in plant Isoprenoids Ketones essential oils e.g. Nootkatone C15 – Sesquiterpenes Obtained via: e.g. Valencene, Beta‐Bisabolene  Plant material extraction Alcohols (often as side products) e.g. Santalol  Chemical synthesis (only for simple isoprenoids) C20 – Diterpenes  Fermentation Proprietary Material Do not Copy

Fermentative production process  Constant quality & availability Microorganism  Not depending on harvest with terpene synthase Distillation  Scalable  Cost effective supply Glucose  Sustainable & renewable resources  No pesticides Fermentation Proprietary Material Do not Copy

High efficiency – low carbon footprint Traditional valencene production from orange oil:  Inefficient: 160.000 Oranges for 1 kg Valencene  Harvest dependent: Taste – Volume – Price  High environmental footprint – agricultural footprint / pesticide use  Shortages due to Citrus greening disease Proprietary Material Do not Copy

Microbial platform: Rhodobacter Rhodobacter sphaeroides  Natural isoprenoid producer: Carotenoids  High product tolerance  Scalable and robust  No problematic off‐odors Proprietary Material Do not Copy

Rhodobacter terpenoid producer platform Additional heterologous pathway increases FPP pool glucose G-3P DXP Pyr FPP Terpene AcCoA MVA IPP DMAPP act hcs mvaA mvk pmk mvd idi IspA TCA Proprietary Material Do not Copy

Plug‐in terpene synthases Sugar } R. sphaeroides MEP  Plug‐in terpene synthases: produce specific Isoprenoid pathway containing FPP Products terpene or terpene mixture with high yields Heterologous mevalonate pathway genes Terpene synthase  Access to unique and highly active synthases Terpene product  Carbon flux directed to terpenes by tuning down competing pathways Proprietary Material Do not Copy

Bioprocess development & Scale‐up  Own bioprocess development lab  Improvement of product titer, rate and yield  In‐house up‐scaling up to 200 l scale  First industrial scale up in 2010  Specific DSP Solutions Proprietary Material Do not Copy

High quality production  Reliable Scale‐up & robust performance at commercial scale  Established production partnerships  Sufficient fermentation capacity for growth  Established quality systems – ISO 22000 certified  Kosher + Halal approval Proprietary Material Do not Copy

Products & Product Pipeline Products Product Pipeline  Natural aroma compound in oranges  Flavouring agent in beverages, use level ca. 1 ppm  Also used for the production of nootkatone Valencene Citrus ingredients  Grapefruit aroma  High value flavour with low natural abundance  Odor threshold < 1ppm, also used in fragrance Nootkatone Patchouli  Lemon & bergamot aroma component  Pleasant balsamic, green odour Sandalwood  90% pure, pesticide‐free Beta-Bisabolene Proprietary Material Do not Copy

R&D partnerships & Grants Proprietary Material Do not Copy

Click to edit Master title style INNOVATIVE CREATORS OF NATURAL INGREDIENTS THANK YOU FOR YOUR KIND ATTENTION ! Strictly Confidential – subject to confidentiality provisions Distribution and copying not allowed