Introducing the LC-MS/MS Portfolio – TSQ Quantis and Altis Osama Abu-Nimreh CMD Sales Support Specialist MECEC , Dubai The world leader in serving science
Introduction to TSQ Altis and TSQ Quantis TSQ Altis Performance: Sensitivity, Selectivity (H-SRM) Thermo Scientific TM Thermo Scientific TM TSQ Quantis TM TSQ Altis TM High-end Mid-tier Mass Range 5-2000 5-3000 SRM/sec 600 600 Selectivity (H-SRM) 0.2 Da FWHM 0.4 Da FWHM TSQ Quantis Sensitivity 500,000:1 150,000:1 (HESI Reserpine 1 pg) Omics, Research, Pharma/Biopharma, Environmental and Food Safety, Clinical Targeted Market Clinical Research and Forensic Toxicology Research, and Forensic Toxicology Robustness, Reproducibility, Speed, Ease-of-Use, Flexibility 2
TSQ Altis: Sensitivity with Robustness, No Compromises Active Ion Management Plus ( AIM+ ) - The next step in precision design delivers the Active collision cell with axial DC field ultimate in ion management, inception to detection, from the OptaMax TM ion source housing to the facilitates more SRMs/sec enhanced electron multiplier. Incorporates segmented quadrupoles with hyperbolic surface and enhanced RF Electronics to further optimize ion management precision, reliability, speed, and reproducibility. Ion beam guide with neutral blocker Reduces chemical background High capacity Ion transfer tube (HCTT) Increases ion flux Segmented Quadrupoles with hyperbolic surface for enhanced performance with both SRM and H-SRM (0.2 FWHM) NEW! Electrodynamic ion funnel (EDIF) Increases ion flux OptaMax TM NG Enhanced dual-mode electron multiplier detector NEW! APCI ready NEW! Ensures excellent linearity and dynamic range 3
TSQ Quantis: Unprecedented Robustness, Day After Day Active Ion Management Plus ( AIM+ ) - The next step in precision design delivers the ultimate in ion management, inception to detection, from the OptaMax TM ion source housing to the enhanced electron multiplier. Incorporates segmented quadrupoles with hyperbolic surfaces and enhanced RF Electronics to Enhanced dual-mode electron multiplier further optimize ion management precision, reliability, speed, and reproducibility. detector ensures excellent linearity and dynamic range NEW! Stacked ring ion guide (SRIG) Increases ion flux Segmented Quadrupoles with hyperbolic surfaces for enhanced performance with both SRM and H-SRM (0.4 FWHM) NEW! OptaMax TM NG APCI ready NEW! Ion beam guide with neutral blocker Reduces chemical background Active collision cell with axial DC field facilitates more SRMs/sec 4
OptaMax NG Source Housing Benefits: Reliable and consistent performance with improved usability! Re-designed APCI discharge assembly • Built-in to every source (separate APCI sprayer required for APCI mode) • Re-designed on/off switch (to improve usability) Re-designed HESI Sprayer • Needle adjustment is no longer possible during acquisition (locked position) • Tool available to help the user to correctly set needle protrusion Usability and Consistency • Vertical adjustment moved to the side for easier access • New drain insert with improved latching and locating pin to prevent rotation • Improved sprayer alignment and stability • New finer threads on HESI and APCI sprayers to make installation easier 5
Segmented Quadrupoles Benefits: Increased Sensitivity (more significant at higher mass range) Flat tuning for consistent and robust performance • The use of RF only pre-filters (segments) between the entrance lens and the quadrupole minimizes the effects of fringe fields, leading to improved transmission (and therefore sensitivity) at unit and higher resolution. • With the RF only pre-filter, the tuning of several lenses is flat across mass range allowing the voltage to be set and not tuned. This helps reducing the complexity of the tune and making the systems more consistent. 6
Detector Benefits: Increased electron multiplier lifetime. Increased Uptime! • Increased number of dynodes (21) for extended lifetime. • Improved electron multiplier calibration routine. • Excellent linearity and dynamic range across the mass range. • Reduced number of service visits leading to more uptime. 7
RF Circuitry Benefits: More compounds in the same run or longer dwells on existing method • New main RF/DC electronics • Analyze more compounds in the same time window or better Quantitation results with better ion statistics (more scans across your chromatographic peak) • Up to 600 SRM/sec 8
What Makes the new Triple Quads Robust? OptaMax NG Ion Beam Guide ITT/Sweep Cone Robustness The ability to perform at the expected level Re-designed Ion beam guide Ion transfer tube source housing and sweep cone with neutral (LOD, LOQ, MRL) under adverse blocker conditions (complex samples, limited sample preparation) for the desired period of time without maintenance. NEW! Reliable and consistent No need to break vaccum for Blocks neutral keeping the ion performance with improved basic maintenance! path clean while reducing noise! usability! 9
What Makes the new Triple Quads Consistent? OptaMax NG Optics Segmented Quads Consistency The ability to report equivalent results every time independently of the system or the user (day-to-day and system-to- Improved Optics Segmented Quads Re-designed Source system) Alignment Housing with hyperbolic surfaces NEW! NEW! NEW! Reliable and consistent More consistent performance Flat tuning across the mass performance with improved from system-to-system! range! usability! 10
What Makes the new Triple Quads Reliable? Reliability Source housing Increased time between service calls (mean time between failure- MTBF) Increased uptime New RF circuitry design Improved electron multiplier NEW! New front panels Optics Alignment Improved Calibration and Diagnostics 11
Demonstration of Robustness – Food Safety Atrazine QC monitored in leek for more than 400 injections with 4.5% RSD . Red lines represent ± 20% response at 10 μ g/Kg. Yellow lines show the time the system was placed in standby mode for 12h to demonstrate consistent performance after standby period Atrazine (QC 10 μ g/Kg) monitored in Leek 625000 +20% 525000 Area Counts 425000 4.5% RSD 325000 -20% 225000 125000 0 50 100 150 200 250 300 350 400 Number of Matrix Injections 12
13 Demonstration of Robustness – Clinical Research calculated amounts (ng/mL) monitored in crashed whole (Cyclosporin A, Everolimu, blood for more than 1500 Sirolimus and Tacrolimus) Immunosuppressants injections. Red lines represent ± 20% of Calculated Conc. (ng/mL) Calculated Conc. (ng/mL) 100 150 200 250 300 10 15 20 25 30 50 0 5 0 #14 #14 #64 #64 #114 #114 #164 #164 #214 #214 #264 #264 %RSD = 2.36 Cyclosporin A #314 #314 #364 #364 #414 #414 #464 %RSD = 4.08 Sirolimus #464 #514 #514 Injection # (12/day) Injection # (12/day) #564 #564 #614 #614 #664 #664 #714 #714 #764 #764 +/-20% #814 #814 #864 #864 #914 #914 #964 #964 #1014 #1014 #1064 #1064 #1114 #1114 #1164 #1164 #1214 #1214 #1264 #1264 #1314 #1314 #1364 #1364 #1414 #1414 #1464 #1464 Calculated Conc. (ng/mL) Calculated Conc. (ng/mL) 10 15 20 25 30 0 5 10 12 14 16 18 20 0 2 4 6 8 #14 #14 #64 #64 #114 #114 #164 #164 #214 #214 #264 #264 #314 %RSD = 4.22 Everolimus #314 #364 #364 #414 #414 #464 #464 %RSD = 3.57 Tacrolimus #514 Injection # (12/day) #514 #564 Injection # (12/day) #564 #614 #614 #664 #664 #714 #714 #764 #764 #814 +/-20% #814 #864 #864 #914 #914 #964 #964 #1014 #1014 #1064 #1064 #1114 #1114 #1164 #1164 #1214 #1214 #1264 #1264 #1314 #1314 #1364 #1364 #1414 #1414 #1464 #1464
Sensitivity with Reproducibility NEW! NEW! Segmented Optics Quads Alignment Active collision cell NEW! NEW! New Source EM Active Ion Management Plus ( AIM+ ) The next step in precision design delivers the ultimate in ion management, inception to detection, from the OptaMax NG source housing to the enhanced electron multiplier. Incorporates segmented quadrupoles and enhanced RF Electronics to further optimize ion management precision, reliability, speed, and reproducibility. 14
TSQ Altis: Collaboration with Beatson Institute for Cancer Research LPA 17:0 @ 0.01 ng/mL Glutamic Acid TSQ Quantiva TSQ Altis NL:6.7E6 (TSQ Altis) 2.5 x Greater! NL:6.7E6 (Aurora) 2x Greater! NL:2.7E6 (TSQ Quantiva) RSD 3.4% (n=3) RSD 0.9% (n=3) LPA at 0.01 ng/mL with RSD below 1%! 2 x greater response on TSQ Altis! Confirming Ion detected on TSQ Altis! A 2.5 fold response increase was observed when transferring the metabolomics assay from TSQ Quantiva to TSQ Altis. 15
TSQ Altis: Quantitation of Therapeutic Drugs in Plasma Quantitation of Desmethyldoxepin in plasma 2.5 pg/mL TSQ Quantiva TSQ Altis Compound Name LOQ (pg/mL) LOQ (pg/mL) Desomorphine 5 5 Desmethyldoxepin 10 2.5 TSQ Altis RSD = 4% (n=3) Flecainide 2.5 1 Midazolam 5 2.5 Imipramine 10 2.5 Amitriptyline 10 2.5 TSQ Quantiva Fluoxetine 5 5 Diazepam 5 2.5 1.30 1.40 1.50 1.60 Time (min) ~ 2.5 X average sensitivity improvement over TSQ Quantiva 16
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