Introducing the Agilent 7000A Triple Quadrupole GC/MS Paul - PowerPoint PPT Presentation

Introducing the Agilent 7000A Triple Quadrupole GC/MS Paul Zavitsanos, Joe Weitzel, Monty Benefiel and Terry Sheehan May 2008 Agilent Restricted Page 1

Agilent Restricted What’s a Triple Quad? Page 2

GC/MS Triple Quad (QQQ) Collision Gas (N2 ) Ion Source Detector Quad 1 Hexapole Collision Quad 2 • Ionize Cell Agilent Restricted Page 3

What is MRM MS/MS? Multiple Reaction Monitoring EI: many ions from the source EI-MS/MS Product 2 Product 1 Product 3 isolate precursor Q1 SIM before CID CID + Q3 filter Agilent Restricted Page 4

Why MS/MS? Greater Selectivity Than SIM in complex samples EI-MS/MS EI-SIM Precursor selectivity same as SIM selectivity proportional to High probability that at least one product spectral resolution ions will be a unique dissociation product interference no selectivity against ions of the precursor BUT not the interference with same m/z analyte Product 2 Product 1 interference Product 3 unit mass resolution Precursor Ion The precursor ion should NOT be used for ion ratios or quantitation since the interferences will be the same as the SIM ion Agilent Restricted Page 5

Why a GC/QQQ System? • Allows for the selective quantitation of target compounds in high chemical background samples • Better S/N in complex matrices than can be achieved by single quad approaches • Newer regulations in some markets specify analytical power commensurate with GC/QQQ Agilent Restricted Page 6

Why a Triple? A Picture is worth a thousand words. GC/MS Single Quad 100 fg HCB SIM MS SIM GC/MS/MS QQQ MRM EI 100fg HCB in MS/MS MRM “DIRTY” Matrix S/N: 116:1 RMS Agilent Restricted Page 7

SIM vs MRM for HCB 100 fg HCB in Clean Matrix 300 fg HCB in Diesel Single MS: SIM 283.8 S/N=26:1 RMS S/N=6:1 RMS MS/MS: 283.8:213.9 S/N=37:1 RMS S/N=86:1 RMS Agilent Restricted Page 8

Technology • Agilent took the Best technologies from their industry leading 597X Series GC/MSD: • Heated monolithic gold plated quartz quadrupole • Proven reliable high performance source design • AUTOTUNE • And the Agilent 6410A LC/QQQ: • Linear acceleration enhanced Collision Cell • Wide Mass-Bandwidth QQQ ion optics • Mass Hunter software Agilent Restricted Page 9

Why Proprietary Heated Quartz “Gold” Quads? • Heated monolithic gold plated hyperbolic quartz quadrupole is the “gold” standard in quadrupole instruments for GC/MS • High 200C temperature quadrupole operation results in greater reliability with “dirty” samples • Virtually eliminates the need to clean quads as a maintenance issue • More stable tunes and methods over a longer period of time in real world sample environments • High speed MRMs (500 MRMs/sec) possible with Agilent Quad electronics • AUTOTUNE Friendly!!! Agilent Restricted Page 10

Agilent Restricted Agilent 7000A Analyzer Page 11

Agilent Restricted Analyzer Details Page 12

High Performance Differentially Pumped Manifold N 2 (1 sccm) He (1-5 sccm) P1 P2 Collision Cell He or H 2 (1-8 sccm) 5-20E-5 Torr 8E-5 Torr CH 4 (0-2 sccm) Source Analyzer Edwards Edwards Rough Pump Split Flow Turbo Split Flo Turbo Agilent Restricted Page 13

Agilent Restricted Analyzer Details Page 14

Agilent Restricted Analyzer Doors Open Page 15

Proven source performance • Inert source design proven over thousands of systems in current use • Stay-Clean design extends maintenance intervals with dirty samples • Source tune parameters in the Autotune file • Dual Filament design reduces maintenance intervals Agilent Restricted Page 16

Agilent Restricted EI Source Details Page 17

High Performance Collision Cell Design • Linear acceleration design is optimized for high speed performance without ion ghosting or cross-talk • MRM speed to 500 MRMs/sec allows determination of more compounds per ion group • High sensitivity with wide mass bandwidth eliminates the need to “tune on my your Compound” for optimum sensitivity • Helium seeded chemical noise reduction collision cell increases S/N. Agilent Restricted Page 18

Agilent Restricted Q1-Collision Cell Interface Page 19

Agilent Restricted Collision Cell Details (2 rods removed for clarity) Page 20

High performance triple off-axis detector • Ultra low neutrals noise • Long life and high linearity • Gain calibration corrects tune file for detector age to allow repeatable long term method performance. • Same design as used in the 5975 GC/MSD Agilent Restricted Page 21

Triple-Axis Detector Offset shield for Z steering rod secondary particles hyperbolic quartz transmission high energy quadrupole dynode analyzer Y triple channel electron ion beam multiplier X Agilent Restricted Page 22

Agilent Restricted Q2-Detector Interface Page 23

Autotune • Proprietary program tunes the source, mass analyzer, and detector for (as applicable): – Ion transmission – Mass axis calibration – Mass resolution – Detector gain vs Voltage • Autotune settings are saved with the method for repeatable method performance. • Manual Tune override is available Agilent Restricted Page 24

Agilent Restricted Performance Page 25

Agilent 7000A 100 fg HCB Sensitivity + MRM (283.8 -> 248.9) 100fgHCB_newvial_methHCB_MRM_PW1p21p2__527N2DAC_600HeDAC_35VColE_1e7Gain_2040EMV_0Baseline_12.d Noise (RMS) = 1.99; SNR (6.387min) = 146.3 2 x10 3.4 6.387 3.2 3 2.8 2.6 2.4 2.2 146:1 RMS S/N vs Spec of 100:1 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 Abundance vs. Acquisition Time (min) 1pg HCB Benchmark comparison Agilent GC/QQQ >1000:1 Competitor 1 100:1 Agilent Restricted Page 26

Agilent 7000A OFN Sensitivity + MRM (272.0 -> 241.0) 100fgOFN_MRM_N2DAC1160_He18p5pct_30VColE_1e7Gain_2410EMV_GaugeOff_02.d Noise (RMS) = 1.10; SNR (5.471min) = 186.3 2 x10 5.471 2.4 1 1 2.3 100fg OFN in clean Matrix 2.2 2.1 2 1.9 272:241 Transition 1.8 1.7 186:1 RMS S/N 1.6 1.5 1.4 1.3 1.2 1.1 1 0.9 0.8 0.7 0.6 0.5 0.226 7.121 6.025 0.4 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 4.4 4.6 4.8 5 5.2 5.4 5.6 5.8 6 6.2 6.4 6.6 6.8 7 7.2 Abundance vs. Acquisition Time (min) Agilent Restricted Page 27

Agilent Restricted Innovation that led to this level of GC/QQQ sensitivity Page 28

Helium Seeded Collision Cell 1 ml/min N2 Collision Gas Q1 Collision Cell Ions He* + Ions Out In +. + He He* + N 2 N 2 +. + A A +. + N 2 N 2 Agilent Restricted Page 29

Helium Seeded Collision Cell 1 ml/min N2 Collision Gas Q1 Collision Cell Ions He* + Ions Out In 2.5 ml/min He Buffer Gas He* + He 2He + Heat +. + He He* + N 2 N 2 Agilent Restricted Page 30

Effect of Helium Buffer Gas Tested with HCB S/N 341:1 Helium Buffer Gas OFF Agilent Restricted Page 31

Effect of Helium Buffer Gas tested with Hexachlorobenzene Noise Comparison S/N 341:1 Helium Buffer Gas OFF S/N 1294:1 Helium Buffer Gas ON Agilent Restricted Page 32

Agilent Restricted Software Page 33

Mass Hunter MS Workstation Software • Modern software interpretation of the proven industry standard GC/MS Chemstation platform • Single software Platform for all Agilent MS Systems – LC/SQ , LC/QQQ, LC/TOF, LC/QTOF – GC/SQ, GC/QQQ – ICP/MS Agilent Restricted Page 34

Agilent Restricted GC/QQQ Software Modules Acquisition Reporting Analysis Excel Page 35

Agilent Restricted Instrument Control UI/navigation Page 36

Agilent Restricted QQQ Acquisition Method Editor Dialog Single window acquisition setup Page 37

Agilent G7000A GC/QQQ • Outstanding sensitivity 100fg of OFN on column at 100:1 S/N RMS in MS/MS mode using AUTOTUNE parameters verified at customer installation. • 1050 amu Mass Range • 500 MRM/sec Speed • Reliable heated monolithic gold plated hyperbolic quadrupoles • Differentially pumped vacuum system • CI/NCI upgrade available • New Helium seeded collision cell technology • Agilent 7890 GC with Capillary Flow technology. • Mass Hunter Software. Agilent Restricted Page 38