Inducing a thermogenic response to cold shock in Escherichia coli - PowerPoint PPT Presentation

Aug 25, 2022 •229 likes •434 views

Inducing a thermogenic response to cold shock in Escherichia coli Georgia Institute of Technology November 18, 2010 Heat generating gene: AOX intermembrane space matrix OmpA Witho thout Om OmpA AOX AOX AOX AOX AOX AOX AOX AOX

Inducing a thermogenic response to cold shock in Escherichia coli Georgia Institute of Technology November 18, 2010
Heat generating gene: AOX intermembrane space matrix
OmpA Witho thout Om OmpA AOX AOX AOX AOX AOX AOX AOX AOX AOX AOX With th Om OmpA AOX AOX
Generating Heat Output Cold Shock Response RN RNA Polym ymera rase
Temperature Profile In E. coli (K) 288.102 T ( x ) E . coli = − 25091.379 x 2 + 3.517 x + 288.102 288.096 Temperature Profile in Agarose (K) 288.150 T ( x ) Agarose = − 21.586 x + 288.107 288.000
288. 288.06 065 288. 288.00 000
HybB-mRFP HybB-OmpA-mRFP
Absolute Te Tempe mperature b by Strai rain at n at 15 15 ° C 16.5 e (C) 16.3 erature ( 16.1 15.9 AOX1a 15.7 Temper RFP control 15.5 15.3 15.1 14.9 14.7 14.5 Minut Min utes
Absolute Te Tempe mperature b by Strai rain at n at 25 25 ° C 25.5 e (C) 25 erature ( 24.5 AOX1a 24 Temper RFP control 23.5 23 22.5 22 21.5 Min Minut utes
Heat at Pro Production Above Base aseline ne A After C Cold S Shock ck 2.5 2 1.5 (C) AOX1a (15 C) ees ( RFP control (15 C) Degrees 1 AOX1a (20 C) RFP control (20 C) 0.5 0 -0.5 Minut Min utes
Conclusion Experiments Construction Modeling Project Design
Dr. E Eric ic Ga Gauche ucher Dr. M Mar ark Sty Styczynski Below: Richard Joh, Scott Holmes, Christian Mandrycky, Sid Tantia, Mitesh Agrawal, Rob Fee, Dr. Mark Styczynski, Shadeah Suleiman, Amy Schwartz, Debika Mitra, Atta Hassan, Monica Huynh, Margo Clark, Christina Graves, Dr. Jos oshua hua We Weit itz Ryan an Randal all Gita Mahmoudabadi [not pictured] Dr. . Mega gan C Cole Richa ichard d Joh oh

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