Geno2pheno[HCV] - Past, Present & Future Developments Bastian - PowerPoint PPT Presentation

Geno2pheno[HCV] - Past, Present & Future Developments Bastian Beggel Max-Planck-Institute for Informatics D-66123 Saarbrücken, Germany

geno2pheno[HCV] geno2pheno[HCV] • web-service for the analysis of HCV sequence data • (sub)genotype • drug resistance • rules based analysis • requires drug resistance relevant part of NS3, NS5A, NS5B Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] – Hit Counts • g2p[HCV] started in 2011 with two NS3 inhibitors • Currently 9 drugs are supported DSV, OMV, PTV SOF TCV, BCP Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] How to find g2p[HCV]? http://www.genafor.org Bastian Beggel geno2pheno[HCV]

Geno2pheno[HCV] – Input Page How to find g2p[HCV]? How to find g2p[HCV]? http://www.genafor.org identifier upload files paste sequence options align and predict Bastian Beggel geno2pheno[HCV]

Geno2pheno[HCV] – Result Page How to find g2p[HCV]? http://www.genafor.org Sequence Information Resistance Summary Mutation- level Analysis Bastian Beggel geno2pheno[HCV]

Geno2pheno[hbv] – Output Options PDF CSV Bastian Beggel geno2pheno[HCV]

What else? • (Sub)genotype specific rule sets • Extended genotype reference set (accounting for 7 genotypes and 87 subgenotypes) • Many more small changes to improve usability Bastian Beggel geno2pheno[HCV]

What’s next? Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] – Present Developments Therapy Outcome Data Phenotypic Assay Fold changes Genome Rep. cycle WT virus Cell culture Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] – Future Developments Next Gen. Sequencing Sanger Sequencing Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] – Future Developments Score Drug Combinations Individual Drug Resistance Boceprevir resistant Paritaprevir resistant Simeprevir susceptible Telaprevir resistant Daclatasvir resistant Ledipasvir resistant Ombitasvir susceptible Dasabuvir susceptible Sofosbuvir susceptible Bastian Beggel geno2pheno[HCV]

Acknowledgements • Thomas Lengauer • Rolf Kaiser • Joachim Büch • Elena Knops, • Alex Thielen • Saleta Sierra Aragon • Mathias Döring • Anna Sikorski • Sandra Ciesek (Hannover Medical School) • Jörg Timm, • Thomas von Hahn (Hannover Medical School) • Andreas Walker • Daniel Rupp (University Hospital Heidelberg) • Verena Keitel • Ralf Bartenschlager (University Hospital Heidelberg) • Hanaa Gaber (Technische Universität; München) • Ulrike Protzer (Technische Universität; München) • Christoph Sarrazin (Universtiy Hospital Frankfurt) Bastian Beggel geno2pheno[HCV]

Thank You! Bastian Beggel geno2pheno[HCV]

geno2pheno[HCV] How to find g2p[HCV]? http://www.genafor.org Genotyping Result: Bastian Beggel geno2pheno[HCV]