Experiences from the Sussex Ouse Sarah Purnell The University of - - PowerPoint PPT Presentation
Experiences from the Sussex Ouse Sarah Purnell The University of Brighton The University of Brighton partner: The Environment & Public Health Research Unit (EPHRU) has specialist facilities used for research into the microbial quality of
Sarah Purnell The University of Brighton
The Environment & Public Health Research Unit (EPHRU) has specialist facilities used for research into the microbial quality of fresh, estuarine, and marine (aquatic and terrestrial) environments. Active on the AquaManche project: Professor Huw Taylor
Miss Sarah Purnell
(65% agriculture)
Newhaven, Lewes, Uckfield and Haywards Heath
26 monthly sampling sites
32 visits (2009-2011), 853 samples analysed for:
Intestinal enterococci Faecal coliforms (E.coli)
Somatic coliphage (general faecal indicator) GB124 (human-specific marker)
(Burbano-Rosero et al., 2011) (Diston, 2010, da Silva, 2012)
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 1 2 3 4 5 6 6b 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Mean CFU/100ml (Log10) Site Number
Mean intestinal enterococci (Log10 CFU/100ml) Mean faecal coliforms (Log10 CFU/100ml) The EU Bathing Water Directive sufficient quality standard for inland bathing waters for both E.coli and intestinal enterococci The EU Bathing Water Directive excellent quality standard for inland bathing waters for both E.coli and intestinal enterococci
The map shows AquaManche water quality monitoring sites in the River Ouse catchment. The pie charts show the percentage of samples that failed to meet ‘sufficient’ water quality classification according to the EU Bathing Water Directive. Although these sites are not designated as bathing water sites, these results illustrate the level of faecal contamination present within the River Ouse catchment.
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 1 2 3 4 5 6 6b 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Mean somatic coliphage (Log10 PFU/100ml) Site Number 0.0 1.0 2.0 3.0 4.0 5.0 6.0 1 2 3 4 5 6 6b 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Mean GB124 phage (Log10 PFU/100ml) Site Number
Human specific marker GB124 Human specific marker GB124 marker never detected at these sites, animal sources may be dominant faecal contributors at these sites Directly downstream of waste water treatment works Somatic Coliphage
between chemo-physical and microbial parameters for the whole data set.
moderate to strong, significant relationships between faecal indicator
enterococci, and somatic coliphage) and temperature, conductivity, turbidity and rainfall (previous 24h prior to sampling) (p=<0.01).
(p<0.01). Moderate correlations existed between faecal indicator bacteria and turbidity (p=<0.01).
Spring Neap
There was significant correlation between rainfall and faecal indicator organisms and chemo-physical parameters such as turbidity (P=<0.01: Rs=0.837).
SWAT is a river basin
Results of the model
The River Ouse currently receives high loads of faecal pollution in relation to current legislation. Bacteriophage of the human-specific marker GB124 were detected at all sampling sites with the exception of four, which appeared to be dominated by non-human faecal inputs. Detected concentrations of GB124 were low throughout much of the two year sampling period. Concentrations reached their highest levels after heavy rainfall. One storm event in particular resulted in GB124 levels in access of 85,000 PFU/100ml. These bacteriophages have been shown to be co-present with human adenovirus and norovirus so may offer an insight into the potential human health risk. The combination of monitoring, MST and modelling within the River Ouse catchment (as part of an ARMT) may offer risk prediction and inform future management within the catchment.
Focus on developing the model
Examine the potential of
Assess the potential impacts of
This work was supported by the European Regional
We would like to thank Morgane Bougeard for her help with
We would also like to thank Southern Water, the Environment
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Purnell, S.E., Ebdon, J.E., and Taylor, H.D. (2011). Bacteriophage lysis of
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