Down-Regulation of miRNAs by Mutated FOXP2 Ellen Korcovelos May - PowerPoint PPT Presentation

Down-Regulation of miRNAs by Mutated FOXP2 Ellen Korcovelos May 11, 2016 BNFO 300

Issue at hand World Dementia Prevalence Dementia Number of People (millions) 81 62 Neurodegeneration Memory Loss Speech Deficiencies 42 33 Alzheimer’s Disease 24 Mild Cognitive Impairment Frontotemporal Dementia Primary Progressive Aphasia 2000 2010 2020 2030 2040 Year Vascular Dementia

miRNAs and dementia Mature miRNA RISC mRNA miRNA

miRNAs and dementia Mature miRNA RISC mRNA miRNA mRNA decay gene expression

miRNAs and dementia APP BACE1 Mature miRNA FOXP2 RISC mRNA miRNA

miRNAs and dementia A β 42 A β 42 APP A β 42 Mature miRNA RISC mRNA miRNA

miRNAs and dementia FOXP2 ? FOXP2 APP A β 42 Mature miRNA RISC mRNA miRNA

My experiment Cell Preparation Extracting the miRNA Analyzing Expression Murine frontotemporal Buffer lyses tissue TaqMan Low-Density cells Microarray DNA removed by ½ unaltered FOXP2 centrifugation Rt-PCR ½ point mutation affected RNA binding membrane FOXP2 ?

My experiment Cell Preparation Extracting the miRNA Analyzing Expression Murine frontotemporal Buffer lyses tissue TaqMan Low-Density cells Microarray DNA removed by ½ unaltered FOXP2 centrifugation Rt-PCR ½ point mutation affected RNA binding membrane FOXP2 ?

My experiment Cell Preparation Extracting the miRNA Analyzing Expression Murine frontotemporal Buffer lyses tissue TaqMan Low-Density cells Microarray DNA removed by ½ unaltered FOXP2 centrifugation Rt-PCR ½ point mutation affected RNA binding membrane FOXP2 ?

My experiment Cell Preparation Extracting the miRNA Analyzing Expression Murine frontotemporal Buffer lyses tissue TaqMan Low-Density cells Microarray DNA removed by ½ unaltered FOXP2 centrifugation Rt-PCR ½ point mutation affected RNA binding membrane FOXP2 ?

Possible Results

Thank you.