The F The First MDPI– rst MDPI– Lincoln Lincoln University University University University DIFFERENTIAL AROMA VOL DIFFERENTIAL AROMA VOL DIFFERENTIAL AROMA VOLATILES DIFFERENTIAL AROMA VOLATILES TILES TILES International Internati nal Conference on Conference on IN NON IN NON IN NON IN NON-CLIMACTERIC NEAR IN NON IN NON IN NON IN NON-CLIMACTERIC NEAR CLIMACTERIC NEAR CLIMACTERIC NEAR- CLIMACTERIC NEAR- CLIMACTERIC NEAR CLIMACTERIC NEAR CLIMACTERIC NEAR Foo F F oods d d ISOGENIC LINES OF MELON AS ISOGENIC LINES OF ISOGENIC LINES OF MELON AS ISOGENIC LINES OF MELON AS MELON AS BIOMARKERS OF DIFFERENCES OF BIOMARKERS OF DIFFERENCES OF BIOMARKERS OF DIFFERENCES OF BIOMARKERS OF DIFFERENCES OF FLESH FIRMNESS A FLESH FIRMNESS A FLESH FIRMNES FLESH FIRMNES FLESH FIRMNESS A FLESH FIRMNES FLESH FIRMNES FLESH FIRMNESS A AT HAR AT HAR AT HAR AT HAR HARVEST HARVEST HARVEST HARVEST EST EST EST EST 10 10-12 APRIL 2013 10 10 12 APRIL 2013 12 APRIL 2013 12 APRIL 2013 T echnical University of Cartagena chnical University of Cartagena (UPCT). (UPCT). Spain Spain N. Dos-Santos N. Dos-Santos M.C. Bueso M.C. Bueso J P F J P Fernández Trujillo J.P. Fernández Fernández-Trujillo Trujillo Trujillo SEEDS SEEDS SEEDS SEEDS SEEDS SEEDS SEEDS SEEDS ME MELO ME MELO LON LON TRANSPL TRANSPLANT TRANSPL TRANSPLANT TRANSPLANT TRANSPLANT TRANSPL TRANSPL NT NT NT NT GROWTH GROWTH GROWTH GROWTH GROWTH GROWTH GROWTH GROWTH ‘Piel de ‘Piel de Piel de Piel de de Sapo de Sapo de Sapo de Sapo Sapo Sapo’ Sapo Sapo’ ME MELO MELO ME ME MELO ME MELO LON LON LON LON JUICE JUICE JUICE JUICE CYLI CYLIND CYLI CYLIND NDERS NDERS SAMP SAMPLE SAMPLE SAMP LE LE HOLD HOLDER HOLD HOLDER GC/MS GC/MS GC/MS GC/MS
Table Table Table Table 1 1. 1. . Main Main aroma oma vola olatiles iles iden entifie fied in the the near ear-isog isogen enic ic li line ne (NILs (NILs SC7-1 an and SC10-2) 0-2) an and the he parental parental line ine ‘Piel Piel de de Sa Sapo’ po’ (PS). PS). The The compounds ompounds were were arrange arranged accor arranged accor arrange according according ing to ing to to th to th the rando the rando random forest random forest rest (R rest (R (RF) (RF) F) variable F) variable variable impor variable impor importance importance ance. Data ance. Data Data were Data were were the were the the mea the mea mean relative mean relative relative content relative content content in content in in pe in pe percentage percentage rcentage of rcentage of of th of th the pr the pr probable probable able aromatic able aromatic aromatic compoun aromatic compoun compounds compounds id identified ed. Gr Group o of compound co Order CAS Nu Number IUPAC Na Name Or PS SC SC10-2 -2 SC SC7-1 1 0006 1 0006 00-14 00 146 -6 pentane-2 t 23di ,3 -dion e KET KET 0,00 0 00 0 00 0,00 0 17 * 0 1 0,17 17 * 2 00014 0-11-4 benzyl acetate ACE 0,3 1 0,3 1 1, 1,76 * 3 00007 8 -8 4 -2 2 -m ethylpropanal ALD 0,00 0, 0,06 06 * 0,03 4 015 7 6 4 -16 -6 2 ,4 -dim ethylbenzaldehyde ALD 0,00 0,73 * 0, 0,00 5 002 5 002 04 04 0 075 0-07 -5 1 (245trim 1-(2 ,4 ,5 -trim ethylphenyl)ethan ethylphenyl)ethan on on e e KET KET 0 00 0,00 0 12 * 0 1 0,12 12 * 0 00 0,00 6 00009 6 -17 -3 2 -m ethylbutan al ALD 0,00 0, 0,08 08 * 0,02 7 000111-8 7 -5 octan -1-ol ALC 0,09 0,2 1 0,5 8 8 06 8 9 2 0-6 4 -9 m ethyldisulfan ylm ethan e SD C 0,2 7 0,3 5 0,52 * 0, 9 0005 9 0005 8 8 3 3 -5 -5 7 7 -3 -3 (1R2 (1R ,2 S)-1,2 S)-1 2 -dim -dim ethylcyclohexane ethylcyclohexane ALK ALK 0 00 0,00 0 14 * 0 1 0,14 14 * 0 00 0,00 10 019 7 8 0-3 9 -3 3 -ethylheptan-2 -ol ALC 0,06 0,00 0, 0,49 * 11 0015 3 4 -08 -3 1-m ethylsulfa n ylethan on e SD C 0,09 0,00 0, 0,42 * 12 00009 8 -8 6 -2 1-phenylethanon e KET 1,7 0 0, 0,00 00 * 0,6 9 13 0006 13 0006 2 2 95 9 -5 9 94 -4 tetradecan tetradecan e e ALK ALK 0 15 0,15 0 42 * 0 4 0,42 42 0,2 0 2 2 2 14 005 4 4 1-5 2 -1 3 ,5 -dim ethylcyclohexan -1-ol ALC 0,3 3 0, 0,09 09 * 0,2 9 15 000112 -4 0-3 dodecane ALK 0,04 0,09 0,11 16 018 8 2 9 -5 5 -5 (E)-hept-2 -enal ALD 0,4 0 0,4 9 0,5 3 17 N 17 N D D 3m 3 -m ethyloctan ethyloctan2ol -2 -ol ALC ALC 0,3 0,3 6 6 0,7 0,7 1 1 0,3 0,3 8 8 18 07 4 3 6 7 -3 3 -2 (1-hydroxy-2 ,4 ,4 -trim ethyl-pen tan-3 -yl) 2 -m ethylpropan oate N AE 1,2 1 0,7 8 0,5 9 19 0006 2 4 -16 -8 decan-4 -one KET 0,15 0,4 2 0,3 8 0 0005 8 5 -7 4 -0 / 0005 7 7 -16 -2 ethan on e, 1-(3 -m ethylphenyl) / ethanon e, 1-(2 -m ethylphen yl)- 2 KET 0,3 3 0, 0,00 00 * 0,2 2 2 1 003 2 14 -4 1-3 octan e-2 -5 -dione KET 0,05 , 0,11 , 0,12 , 2 0006 17 -9 4 -7 2 -phenylpropan-2 -ol 2 ALC 2 ,7 1 0, 0,23 * 2 ,3 1 3 000111-2 7 -3 hexan-1-ol 2 ALC 0,11 0,07 0,07 4 0103 4 0-2 3 -5 (Z)-n on -3 -en-1-ol 2 ALC 0,2 2 0,2 4 0, 0,50 * 2 5 004 6 2 1-04 -10 4 -propan -2 -ylcyclohexan-1-ol ALC 0,3 7 1, 1,73 * 0,3 9 NIL means within rows highlighted with * and bold showed statistical differences from PS data, according to a Dunnett’s test at P = 0.05. Abbreviations: Acetate Esters (ACE), Alcohols (ALC), Aldehydes (ALD), Alkanes (ALK), Chemical Abstracts Service (CAS), International Union of Pure and Applied Chemistry (IUPAC), Ketones (KET), Non-Acetate Esters (NAE), Not Detected (ND), Others (OTH), Sulphur-Derived Compounds (SDC), Terpenes (TER). Differential aroma volatiles in non-climacteric near-isogenic lines lines of melon as biomarkers of differences of flesh firmness at harvest
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