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Detecting Tuberculosis SCSU iGEM 2016 What is Synthetic Biology? - PowerPoint PPT Presentation

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Detecting Tuberculosis SCSU iGEM 2016 What is Synthetic Biology? -Branch of biology that encompasses biotechnology, math, engineering, physics, chemistry, and computer science -Creating genetic circuits / biological components and systems

  2. Detecting Tuberculosis SCSU iGEM 2016

  3. What is Synthetic Biology? -Branch of biology that encompasses biotechnology, math, engineering, physics, chemistry, and computer science -Creating genetic circuits / biological components and systems ISBN: 978-1-491-90429-9

  4. What is iGEM? -The international genetically engineered machine competition -Teams from around the world present their projects at the “Giant Jamboree” in October in Boston

  5. iGEM Parts Registry -Catalog of standardized parts -All produced by iGEM teams -Available for iGEM labs/teams

  6. Tuberculosis (TB) - ⅓ of the world population is infected with TB -In 2014 9.6 million people were sick with TB -There were 1.5 million TB related deaths in 2014 http://museumofhealthcare.ca/explore/ exhibits/breath/anti-tuberculosis.html *all data taken from the CDC website

  7. Tuberculosis Fatality Across the Globe

  8. Tuberculosis Fatality Across the Globe

  9. Tuberculosis Detection Methods doi: 10.1136/postgradmedj-2015-133278

  10. Our Goal -Breath Test -Fast Results -Distributable -Easy to read http://www.safewaybreathalyzer.com/

  11. Project Overview Tuberculosis

  12. Project Overview VOCs Tuberculosis

  13. Project Overview 1-Methylnaphthalene Tuberculosis

  14. Project Overview 1-Methylnaphthalene Tuberculosis

  15. Project Overview 1-Methylnaphthalene Tuberculosis Indigo Dye

  16. Engineering E. coli Indigo Dye 1-Methylnaphthalene 3-Methylsalicylate http://pigeoncad.org/

  17. Engineering E. coli Indigo Dye 1-Methylnaphthalene 3-Methylsalicylate http://pigeoncad.org/

  18. NahR Transcription Factor http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

  19. NahR Transcription Factor http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

  20. NahR Transcription Factor http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

  21. Engineering E. coli Indigo Dye 1-Methylnaphthalene 3-Methylsalicylate http://pigeoncad.org/

  22. Nah Operon http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show

  23. Nah Operon Enzymes NahC NahA NahB NahD NahE NahF Genes http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show

  24. NahR Transcription Factor http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

  25. NahR Transcription Factor http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

  26. Engineering E. coli Indigo Dye 1-Methylnaphthalene 3-Methylsalicylate http://pigeoncad.org/

  27. Indigo Production Nah Operon Our circuit produces http://www.ncbi.nlm.nih.gov/pubmed/6353574

  28. Nah Operon Enzymes NahC NahA NahB NahD NahE NahF Genes naphthalene 1,2-dioxygenase http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show

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