Con ontrol ol of st stored d products cts pest sts s by - PowerPoint PPT Presentation

Con ontrol ol of st stored d products cts pest sts s by natural al products Vlatka Rozman University of Josip Juraj Strossmayer in Osijek Faculty of Agriculture in Osijek, Croatia

Nat Natural ral pro roduc ucts ts possess ss a spectrum rum of pro roper erti ties: insecticidal activity repellence to pests antifeedancy insect growth regulation toxicity to mites and other pests antifungal, antiviral, antibacterial

Ad Advant antages ages of use • They break down rapidly under the Rapid degradation influence of daylight and sunlight, moisture and rainfall. • Typically kill insects quickly or prevent Quick action their feeding immediately after application. Low toxicity to • They are less stable and therefore have warm-blooded smaller negative impact on beneficial and non-target organisms. organisms • They are not harmful to treated plants Selectivity on (they are not or are slightly phytotoxic) treated plants when applied according to instructions.

Disadv dvanta ntages es of use Rapid • Their rapid degradation, though favorable from the standpoint of environmental protection and degradation human health, is often requiring their frequent use. • Although deemed to be considerably less toxic in Toxicity comparison to synthetic insecticides, certain botanical insecticides such as nicotine and rotenone are toxic to humans and fish. Price and • They are generally more expensive than synthetic insecticides, many are not commercially available availability for a number of reasons including standardization of their production.

The main n criteria eria fo for apply lying ing plant nts with insecti ticida idal l pro roper erti ties es • When choosing the appropriate plant species which is found to have insecticidal 1. substances, strict attention should be paid to preservation of ecosystems. • The plant should be widely spread in large numbers in nature. 2. • Leaves, flowers and fruits, which means parts of plants that are regularly renewed 3. should be used mostly, and the root of the annuals if root is needed. • If using parts of perennial plants special care should be taken in order not to 4. exterminate the species (avoid using root of perennial plants). • Selected plants should occupy small area for cultivation, and be undemanding in 5. terms of tillage, water and nutrients. • The selected plant is preferred to be used in other areas such as health care, 6. pharmaceutical industry, food industry, etc. • Also, it is preferable that the plant does not have high economic value. 7. • Active substance of the selected plant should be effective at lower concentrations. 8.

More e significan icant t use of natural al produ ducts ts in practic tice will l be possib ible le if they are compatib atible le with h the following ing principles iples:  safe  low toxicity  easy for application  minimal (or nil) problems with insecticide residues on foods  high efficacy at very low concentrations against stored grain insects, adult pests and their progeny  wide spectrum of efficacy against stored grain insect pests and field pests  low adverse effects on grain handling and quality properties  acceptable in terms of price

Ong ngoi oing researc arch in n Cro roatia tia Croatian Science Foundation (HRZZ) - call: IP-11-2013 • Approved funding a three-year research project Project title: Development of new natural insecticide formulations based on inert dusts and botanicals to replace synthetic, conventional insecticides • Project acronym: DIACROMIXPEST • Project No. IP-11-2013-5570 • Period: from 1 July 2014 to 30 June 2017 • Project leader: Prof. Ph. D. Vlatka Rozman (FoA Osijek)

DIA - DE and inert dusts CRO - DIACROMIXPEST PEST Croatian origin MIX - mixture of substances

Prof. Vlatka Rozman Assist . Prof. Anita Liška Prof. Josip Halamić Assoc.Prof . Renata Baličević Ph.D . Ines Galović Ph . D. Zlatko Korunić Pavo Lucić, MSc. Ing. Agr.

DIACROMIXPEST Project idea: Create a new Croatian product Apply in agriculture and Develop new related sectors technology of Use mixing substances substances of Croatian origin Develop natural insecticide formulations

DIACROMIXPEST Slow the emergence of Safe and slightly resistance due to at toxic to warm- least two blooded organisms mechanisms of action No negative effect on the Based on Croatian treated products and the inert dusts and handling of working botanicals with the goods New natural insecticide Effective on Reasonable price formulation harmful insects for users properties

DIACROMIXPEST New formulations should be 5 to 10 times less used in relation to DE when used alone. New formulations should provide complete protection with 90-99% insect mortality in stored products within 7 to 21 days. New formulations should become one of the bases of integrated protection measures.

Project activities : DIACROMIXPEST Phase I Phase II Phase III Field trials deposits of inert dusts Develop formulations of Test the stability of the new and diatomite in Croatia insecticides formulations under normal storage conditions and on the field Physical and chemical analysis of Select the best formulation for samples the safety of warm-blooded organisms and efficiency to Toxicological analysis of the new Determine the species of diatoms insects formulation of possible acute, oral, dermal and inhalatory toxicity Select samples to test the Test the long-term activity of effectiveness of the insects selected formulations on wheat Preliminary test formulation for and maize pests in public health and Select Croatian aromatic and communal hygiene, protection of food grade plants with plants in vegetation, animal health insecticidal activity Analyze possible residues on and food industry treated wheat and maize Investigate the methods and To give an answer whether Croatia modes of mixing tested plant Chemical analysis of selected has a new formulations of components and inert dusts formulations insecticides from its own resources

Zagreb and the Zagorje region: 6 locality 21 samples Službeni posjet HRZZ 13.5.2015.

Banovina region: 6 locality 23 samples Službeni posjet HRZZ 13.5.2015.

Po žeška gora – Slavonia region: 2 locality 12 samples Službeni posjet HRZZ 13.5.2015.

Službeni posjet HRZZ 13.5.2015.

Službeni posjet HRZZ 13.5.2015.

Collecting and preliminarily test aromatic and food plants with a potential insecticidal activity: • laurel ( Laurus nobilis L.), • lavender ( Lavandula x intermedia ), • lemon balm ( Melissa officinalis L.), • mint ( Mentha piperita L.), • basil ( Ocimum basilicum L.), • marjoram ( Origanum marjorana L.), • oregano ( Origanum vulgare L.), • woodland sage ( Salvia nemorosa L.), • sage (Salvia officinalis L.), • clary sage (Salvia sclarea L.), • savory ( Satureja hortenzis L.), • wild thyme ( Thymus serpyllum L.), • garden thyme ( Thymus vulgaris L.), • sunflower ( Helianthus annuus L.), • rapeseed ( Brassica napus L.).

Službeni posjet HRZZ 13.5.2015.

DIACROMIXPEST Current ently ly status of research ch Currently, research on the project is in the first “ selective ” phase. There are several potential Croatian samples of inert dusts and diatomite (Liška et al ., 2015). Being tested formulations of powder and extracts from Croatian aromatic and food plant. Some of them are very potential, such as lavender (Luci ć et al., 2015.).

Ackn knowled wledgement gement  Financial support was provided by the Croatian Science Foundation (HRZZ):  Scientific research project IP-11-2013- 5570: “Development of new natural insecticide formulations based on inert dusts and botanicals to replace synthetic, conventional insecticides“  www.diacromixpest.eu

Thank nk you for your attentio tion!