CIMMYT CAGE meeting CIMMYT CAGE meeting Update : Identification and utilization of novel sources of Update : Identification and utilization of novel sources of Identification and utilization of novel sources of Identification and utilization of novel sources of resistance against soil borne pathogens in w heat resistance against soil borne pathogens in w heat resistance against soil borne pathogens in w heat resistance against soil borne pathogens in w heat presented at CIMMYT CAGE GRDC Australia/CIMMYT Germplasm Germplasm Meeting Meeting presented at CIMMYT CAGE GRDC Australia/CIMMYT By Dr Julie By Dr Julie Nicol Nicol CIMMYT Pathologist in collaboration with Dr Yann Manes Spring W CIMMYT Pathologist in collaboration with Dr Yann Manes Spring Wheat Breeder. heat Breeder. Adelaide, 5th March 2009 Adelaide, 5th March 2009 CIMMYT MR International Maize and Wheat Improvement Center
Specific objectives of new Specific objectives of new Specific objectives of new GRDC project GRDC project GRDC project Further confirmation and validation of newly identified novel Further confirmation and validation of newly identified novel Further confirmation and validation of newly identified novel sources of soil borne pathogen (SBP) resistance in high yielding sources of soil borne pathogen (SBP) resistance in high yielding sources of soil borne pathogen (SBP) resistance in high yielding advanced germplasm advanced germplasm, with specific emphasis on multiple SBP , with specific emphasis on multiple SBP advanced germplasm, with specific emphasis on multiple SBP resistance. resistance. resistance. Breeding of these sources into Australian and CIMMYT bread wheat Breeding of these sources into Australian and CIMMYT bread wheat Breeding of these sources into Australian and CIMMYT bread wheat backgrounds, and delivery of SBP disease resistance germplasm germplasm backgrounds, and delivery of SBP disease resistance backgrounds, and delivery of SBP disease resistance germplasm through CIMMYT International Nurseries. through CIMMYT International Nurseries. through CIMMYT International Nurseries. Molecular characterization of most promising novel sources of Molecular characterization of most promising novel sources of Molecular characterization of most promising novel sources of resistance for specific SBP with key linkages of useful populations resistance for specific SBP with key linkages of useful populati ons resistance for specific SBP with key linkages of useful populations with the AWBMMP. with the AWBMMP. with the AWBMMP. Production of new mapping populations for Root Lesion Nematode Production of new mapping populations for Root Lesion Nematode CIMMYT MR Production of new mapping populations for Root Lesion Nematode and Crown Rot. and Crown Rot. and Crown Rot. International Maize and Wheat Improvement Center
Methods used for first tw o Methods used for first tw o Methods used for first tw o objectives objectives objectives At least 2 years of field or greenhouse data are At least 2 years of field or greenhouse data are At least 2 years of field or greenhouse data are required for the SBP trait of interest to promote required for the SBP trait of interest to promote required for the SBP trait of interest to promote the line for distribution. the line for distribution. the line for distribution. Key objective is the identification of multiple Key objective is the identification of multiple Key objective is the identification of multiple SBP resistant materials SBP resistant materials SBP resistant materials Emphasis is placed on screening the high yield Emphasis is placed on screening the high yield Emphasis is placed on screening the high yield advanced lines from both the SW and WW advanced lines from both the SW and WW advanced lines from both the SW and WW international programs. international programs. international programs. Australian colleagues are welcome to share Australian colleagues are welcome to share Australian colleagues are welcome to share germplasm for screening SBP in Turkey for screening SBP in Turkey germplasm germplasm for screening SBP in Turkey CIMMYT MR International Maize and Wheat Improvement Center
Germplasm for SBP sent Dec 2008 for SBP sent Dec 2008 Germplasm Germplasm for SBP sent Dec 2008 50 entries sent in Dec08 50 entries sent in Dec08 50 entries sent in Dec08 Currently under increase Currently under increase Currently under increase with AWCC with AWCC with AWCC All pedigree lists and trait All pedigree lists and trait All pedigree lists and trait data on the CIMMYT- - data on the CIMMYT data on the CIMMYT- CAGE website CAGE website CAGE website CIMMYT MR International Maize and Wheat Improvement Center
Spring w heat SBP materials Spring w heat SBP materials Spring w heat SBP materials CIMMYT MR International Maize and Wheat Improvement Center
Winter Wheat SBP materials Winter Wheat SBP materials Winter Wheat SBP materials CIMMYT MR International Maize and Wheat Improvement Center
Progress in understanding the chromosomal location of Progress in understanding the chromosomal location of Progress in understanding the chromosomal location of resistance to P. P. thornei thornei resistance to resistance to P. thornei Source actively P. thornei Chromosomal location Group used in resistance breeding programs 1 2 3 4 5 6 7 DS, 1 Sythethic hexaploid CPI 133872 DS AL, QDPI - Zwart et al. limited LRC AS 2 middle eastern landraces 2 - Iraq 43 (AUS4926) and Morocco CSIRO - Schmidt et al. unknown B BS B DS 426 (AUS13124) extensively in 3 GS50a wheat line DS CSIRO - Lagudah et al. Northern Region and CIMMYT Int. CIMMYT synthetic hexaploid CSIRO/CIMMYT - moderately in 4 CROC_1/AE.SQUARROSA B McIntyre et al. CIMMYT Int. (224)//OPATA B D extensively in CIMMYT Iraqui landrace AUS4930 CSIRO/CIMMYT - CIMMYT Int. and to a 5 B 7.2 McIntyre et al. limited degree in Southern Region B B CIMMYT MR D International Maize and Wheat Improvement Center Toktay et al., 2006 Genome 49: 1-5. Joint collaboration Turkey, CSIRO & CIMMYT
F6 RLN Spring Wheat F6 RLN Spring Wheat F6 RLN Spring Wheat CIMMYT MR International Maize and Wheat Improvement Center
Requests for germplasm germplasm sent sent Requests for Requests for germplasm sent Dec 08 Dec 08 Dec 08 Present request for Present request for Present request for SBP germplasm SBP germplasm SBP germplasm from Australian from Australian from Australian collaborators. collaborators. collaborators. Germplasm planned planned Germplasm Germplasm planned for distribution in for distribution in for distribution in 2010 after seed 2010 after seed 2010 after seed increase. increase. increase. CIMMYT MR International Maize and Wheat Improvement Center
Essential to obtain multiple data Essential to obtain multiple data Essential to obtain multiple data (Greenhouse and field) over years (Greenhouse and field) over years (Greenhouse and field) over years Anything with 1 is as good as MR resistant check, while 1* is better. To promote a MR line for CR at least 2 years GH and 2 years reliable field data is needed. Should be able to identify the best lines based on this – eg Croc (SW) and Burbot-6 (WW) Please see my comments regarding limitations and strength of GH vs Fld techniques CIMMYT MR International Maize and Wheat Improvement Center
Mapping populations – – linked w ith linked w ith Mapping populations Mapping populations – linked w ith AWCMMP GA – – joint collaboration w ith ICWIP AWCMMP GA AWCMMP GA – joint collaboration w ith ICWIP joint collaboration w ith ICWIP (Nicol Nicol/Ogbonnaya) /Ogbonnaya) ( (Nicol/Ogbonnaya) 233 DH individuals of an advanced synthetic derivative from CIMMYT Mexico CROC_1/AE.SQUARROSA (224)//OPATA (CID 72726; SID 531; SH: CMBW91Y00935S-80Y-11KBY-1KBY-010M-1Y-2M-0Y-0SY) x Janz which has resistance to both RLN-Pt and CR. # Furthermore several other valuable disease traits could be explored (FHS, CR, YS, BP, SEPT). This population has been partially mapped for RLN – Pt resistance two resistance loci located on chromosomes 1B and 3B (Toktay et al., 2006 Genome. 49: 1-5). This population belongs to CIMMYT and GRDC and can be utilised without restriction for this project. Planned to be phenotyped for CR and RLN-Pt this year in Turkey and Syria. CIMMYT MR Other new mapping populations are planned for SBP as part of this project International Maize and Wheat Improvement Center
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