CHROMATOGRAPHY CHROMATOGRAPHY is a technique used to separate the - PowerPoint PPT Presentation

CHROMATOGRAPHY

 CHROMATOGRAPHY is a technique used to separate the components present in the mixture  Principle – It is based on the adsorption principle Steps in this technique 1. Adsorption of compound on the stationary phase 2. Desorption of compound from the stationary phase by the mobile phase It is two types

 Adsorption chromatography In this technique components of the mixture are seperated basing on the adsorption phenomenon  Partition chromatography In this technique components of the mixture are seperated basing on the distribution law

Classification

 In adsorption chromatography the stationary phase is a solid. The solutes are adsorped in different parts of the adsorbent column. The adsorbed components are then eluted by passing suitable solvents called eluents,as liquid phase through the column. When a column is used in such type of chromatography. The technique is known as column chromatography  Thin layer chromatography (TLC)is a special type of adsorption chromatography,in which a glass plate coated with a thin layer of the adsorbent is used as a stationary phase