AI powered automated imaging and analysis workflows for EM systems - PowerPoint PPT Presentation

AI powered automated imaging and analysis workflows for EM systems Jérôme Hummel, Guilhem Péretié, David Tourais, Rémi Blanc, Quentin Lavigne, Eric Goergen* The world leader in serving science

Introducing Avizo2D with AI Blurring the line between Imaging & Analysis 2

Avizo2D Software – Overview • Utilize a variety of image processing approaches in a single, easy to modify workflow • The intuitive UI and modular approach enables ability to evaluate the accuracy of processing steps in real time • Attach Avizo 2D directly to your system for on-the-fly analysis 3

Integrated Solutions Our Goal Create a rich, integrated user experience that blends acquisition with advanced analysis Imaging Analysis To ensure you leave our systems with answers Or, are armed with the best data to ask the right questions 4

Avizo2D Software – The Platform Interactive Workflow Player Characterized Output Input Image • Access the full power of Avizo’s unmatched processing libraries in a completely redesigned, intuitive UI • Build recipes on the fly and visualize the impact of each module choice or setting change in real time • Quickly build processing routines for repeated tasks • Batch process images for maximum efficiency • Optimize Imaging conditions/segmentation while on the tool 5

Introducing Avizo2D with AI Why Artificial Intelligence? ü AI is well-suited to solving segmentation on traditionally difficult sample types ü Provides a strong framework for objective, repeatable analysis ü Adds access to cutting edge techniques via easy integration with established models AI in Avizo2D • Dedicated tools for image Labeling and Training of deep learning models • Easy to use application suite to train custom-designed Python derived networks • Any Trained Models can be inserted into analysis workflows (e.g. Keras-Tensor Flow) 6

Avizo2D Software – Deep Learning Models – Step 1: Labeler Cutting-edge technologies wrapped in a user friendly environment • Simple tools for feature labeling • Quickly populate data with target structures 300 nm 7

Avizo2D Software – Deep Learning Models – Step 2: Trainer Easy to implement advanced AI workflows • Choose base topology • Label training set • Input data and Ground Truth • Set up training parameters 8

Avizo2D Software – Deep Learning Models – Step 3: Analyzer using DL model Model applied on a Volume Scope stack Model trained on a TEM stack of neural tissue of a mouse heart muscle 300 nm 2 µm 9

AI Electron Imaging Denoiser 1 µm 1 µm • Enhance image quality using AI-powered denoising tools • Lower dwell time • Quality data on sensitive samples 10

360 µm • Go away from the tool with augmented insight • Link direct to Avizo2D from the microscope • Run image by image to tune results • Batch process as images are obtained • Connect directly to Maps for full automation 11

Avizo2D Software – The Online Recipe Library • Access pre-built workflows • Upload and share workflows • Access pre-trained models • Quickly get up to speed with image processing approaches • Benefit from workflows created by your peers and application experts 12

End-to-End Automation Maps Software System Automation Automated Large Area imaging Templates for quick set up Automate single to thousands of frames Correlative Microscopy Register and co-visualize imagery Import images from any source Import 3D stacks Visualize, Annotate, Share All imagery in one place Use multi-modal data as navigational aids ü Compatible with our full line of Electron Microscopes Annotate and share results with colleagues ü Make the most efficient use of your platform ü Integrated EDS Mapping on SEM and TEM 13

Building Efficient Workflows • Integrated workflows to decrease time to result • Access advanced image processing pipelines while on the tool • Tune and optimize imaging and processing workflows • Automated, end to end experience 14

Avizo2D Software – Connectivity with MAPS | Real-time Data Analysis 15

Classify Au-Ni-Cl Nanoparticles from EDS map Sample courtesy J. Bursik, Institute of Physics of Materials, Brno • Analyzed using pre-built processing recipes • Separated by composition • Full size and shape statistical extraction 16

Characterize porosity in separator polymer - Battery • Repeatable analysis 300 nm • Simple traditional workflow • Live tracking of morphological changes 17

Additive Manufacturing Powder – Statistical Characterization 5 µm • Easily automated workflow • Full morphological characterization • Compensation for focal depth 18

Fluorescence Microscopy: cell count in well plate 40 µm • Avizo2D can be used in optical imaging workflows • Support for import and processing of common optical data outputs • Import and batch process from a single folder or image stack 19

Summary AI Integration Tight System Integration Walk away with ANSWERS • Avizo2D is the digital eco-system for AI-powered, workflow-based imaging data analysis • Low learning curve environment. No high-end image processing expertise required • Unique inter-operability with Thermo Scientific EM acquisition software • Solve analysis cases that are challenging for conventional techniques with AI • Enables repeatable, standardized analysis approaches 20